FastQCFastQC Report
Fri 10 Feb 2017
ERR1630284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462133
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24810.16968360607413963No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG19720.13487145150270188No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT15100.10327377878756583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14730.10074322924111556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.020.0804021
CTTACTC801.6171605E-518.53
CGGCCTT3600.017.98611324
TTACTCG752.0677516E-417.26666619
GACGGAC1502.5102054E-1017.2666667
TAAGAGT1301.3938916E-817.0769234
CTTATAC3400.015.77941337
TCAATAC957.0617745E-515.5789483
TCTGTCG1557.212293E-915.51612958
ATCTCGC3750.015.29333211
TTGGCCG4100.014.89024431
ACGTTAC750.00410511714.79999916
GACGTTA750.00410511714.79999915
GTTATAC750.00410511714.7999993
TCTCGCC4000.014.33750112
TAGACTA1051.6561174E-414.0952385
CGACGTT800.006299701513.87500114
CGTCTAA800.006299701513.8750011
CGTTACT800.006299701513.87500117
ACGGACC2009.840733E-1013.8750018
Produced by FastQC (version 0.11.5)