##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1462133
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72969627250052	31.0	31.0	34.0	30.0	34.0
2	31.926970391886375	33.0	31.0	34.0	30.0	34.0
3	32.0733818332532	33.0	31.0	34.0	30.0	34.0
4	35.73767981435341	37.0	35.0	37.0	33.0	37.0
5	35.65577755238409	37.0	35.0	37.0	33.0	37.0
6	35.721794802524805	37.0	35.0	37.0	33.0	37.0
7	35.69788179324316	37.0	35.0	37.0	33.0	37.0
8	35.672530474313895	37.0	35.0	37.0	33.0	37.0
9	37.370744658659646	39.0	37.0	39.0	34.0	39.0
10	37.27147530354626	39.0	37.0	39.0	34.0	39.0
11	37.361648358938616	39.0	37.0	39.0	34.0	39.0
12	37.23513866385616	39.0	37.0	39.0	34.0	39.0
13	37.26633828796696	39.0	37.0	39.0	34.0	39.0
14	38.466404219041635	40.0	38.0	41.0	34.0	41.0
15	38.46715654458247	40.0	38.0	41.0	34.0	41.0
16	38.45009653704554	40.0	38.0	41.0	34.0	41.0
17	38.40739248755072	40.0	38.0	41.0	34.0	41.0
18	38.3747265125676	40.0	38.0	41.0	34.0	41.0
19	38.39311608451489	40.0	38.0	41.0	34.0	41.0
20	38.34710522230194	40.0	38.0	41.0	34.0	41.0
21	38.299753168829376	40.0	38.0	41.0	34.0	41.0
22	38.28095870895466	40.0	38.0	41.0	34.0	41.0
23	38.234663330900815	40.0	38.0	41.0	34.0	41.0
24	38.22427850270803	40.0	38.0	41.0	34.0	41.0
25	38.18880156593141	40.0	38.0	41.0	33.0	41.0
26	38.03561030357704	40.0	37.0	41.0	33.0	41.0
27	37.93337883762968	40.0	37.0	41.0	33.0	41.0
28	37.86673305369621	40.0	37.0	41.0	33.0	41.0
29	37.805303621489976	40.0	37.0	41.0	33.0	41.0
30	37.76294427387933	40.0	37.0	41.0	33.0	41.0
31	37.664384840503565	40.0	37.0	41.0	32.0	41.0
32	37.57385408851315	40.0	37.0	41.0	32.0	41.0
33	37.524391419932385	40.0	37.0	41.0	32.0	41.0
34	37.43822894360499	40.0	37.0	41.0	32.0	41.0
35	37.36056979768598	39.0	37.0	41.0	31.0	41.0
36	37.27077358899635	39.0	36.0	41.0	31.0	41.0
37	37.22541383034238	39.0	36.0	41.0	31.0	41.0
38	37.12288485384025	39.0	36.0	41.0	31.0	41.0
39	37.036103418772434	39.0	36.0	41.0	31.0	41.0
40	36.952343596649555	39.0	36.0	41.0	30.0	41.0
41	36.84237275268392	39.0	36.0	40.0	30.0	41.0
42	36.81042353876152	39.0	35.0	40.0	30.0	41.0
43	35.61245796381041	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	5.0
11	1.0
12	0.0
13	2.0
14	1.0
15	3.0
16	7.0
17	28.0
18	68.0
19	139.0
20	252.0
21	497.0
22	830.0
23	1457.0
24	2466.0
25	3876.0
26	6000.0
27	8803.0
28	12715.0
29	17479.0
30	23422.0
31	31042.0
32	40568.0
33	52663.0
34	69331.0
35	93434.0
36	129349.0
37	193789.0
38	359224.0
39	414680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.416729531444815	18.591468765153373	12.234933484163205	26.756868219238605
2	18.805334398443918	20.868963356958638	34.35453546291616	25.971166781681283
3	20.234752926033405	22.583239691601243	29.447799892349053	27.7342074900163
4	15.297924333832832	15.180287976538388	33.88638379682286	35.635403892805925
5	14.939817376394624	36.59749147307393	33.76566974413409	14.697021406397365
6	35.64456858575793	34.15434847582265	15.5310084650302	14.670074473389219
7	30.174956724183094	30.070999013085675	20.635879225761265	19.118165036969963
8	27.42821617458877	33.82482988893623	19.85701711130246	18.88993682517254
9	27.42096649210434	14.017466263328988	18.615953541846057	39.945613702720614
10	17.11567962695596	26.695587884275916	32.82690425563201	23.36182823313611
11	36.33404074731916	21.302234475249517	20.66706653909049	21.696658238340834
12	21.904642053766654	25.264596312373772	28.716539466655906	24.114222167203668
13	31.003198751413173	19.17971894485659	24.51890491494276	25.29817738878748
14	23.537804016460882	20.171147221217222	24.03406530048908	32.25698346183282
15	26.08278453464904	27.149308578631352	21.607405071905223	25.160501814814385
16	25.96152333611238	25.926574395078973	22.71045110123361	25.401451167575047
17	24.467815171396857	25.939295536042206	24.467609991703902	25.125279300857034
18	24.1306365426401	24.850133332603804	25.810579475328165	25.208650649427927
19	25.370879393324685	24.968316835746133	25.459175054526504	24.20162871640268
20	25.92452259814942	24.192942776067568	25.541725684325574	24.340808941457446
21	25.533518496607353	24.973241148377063	24.346143613474286	25.147096741541297
22	25.519155918100473	24.540380389472094	24.67853471606208	25.26192897636535
23	24.82359675898157	25.12719431132462	24.78953692995097	25.25967199974284
24	25.57865802905755	25.20659885249837	24.743850251652894	24.47089286679119
25	25.639322824941367	24.777020968680688	24.71177382632086	24.87188238005708
26	25.51525750393432	25.03370076456793	24.468362317244736	24.982679414253013
27	25.493713636173997	24.462890858765928	24.592427638251788	25.450967866808288
28	24.878037770845744	24.739883444255756	25.04642190553116	25.335656879367335
29	24.52971104543841	25.191278768757698	25.12240678515566	25.15660340064823
30	24.62436727712185	25.95208507023643	24.831803946699786	24.591743705941933
31	25.488447357388143	25.19100519583376	24.61985332387683	24.700694122901268
32	24.30934805520428	25.219456779923576	25.090945898902493	25.380249265969653
33	24.568148041252062	24.74261917349516	25.350156244336187	25.339076540916594
34	25.01585013128081	24.427394771884636	25.426072730729693	25.13068236610486
35	25.189705724445037	24.742482387033192	25.34967749171929	24.71813439680248
36	24.677508817597303	25.387909307839983	25.090261966592642	24.844319907970068
37	25.756822395773842	24.34272395192503	24.87721705207392	25.0232366002272
38	24.82038227712527	24.706712727227963	25.738356223407855	24.734548772238917
39	24.86374358556985	24.395455133014575	25.65566880714682	25.085132474268757
40	24.386632406217494	23.90904247424824	26.575557763896988	25.128767355637276
41	23.976614986461563	24.222625438315116	26.808710288325344	24.992049286897977
42	24.598856601964393	23.748591954357092	26.472899524188293	25.179651919490226
43	23.95377164731252	23.530143974590548	26.807342423705645	25.70874195439129
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	42.0
2	60.0
3	135.5
4	211.0
5	211.0
6	289.0
7	367.0
8	377.0
9	387.0
10	563.0
11	739.0
12	739.0
13	1289.0
14	1839.0
15	2848.0
16	3857.0
17	4027.0
18	4197.0
19	4197.0
20	5207.5
21	6218.0
22	7259.0
23	8300.0
24	10239.0
25	12178.0
26	12178.0
27	14597.5
28	17017.0
29	23069.0
30	29121.0
31	32757.5
32	36394.0
33	36394.0
34	41441.0
35	46488.0
36	51656.0
37	56824.0
38	65095.5
39	73367.0
40	73367.0
41	79255.0
42	85143.0
43	84913.5
44	84684.0
45	91586.5
46	98489.0
47	98489.0
48	104749.5
49	111010.0
50	115137.5
51	119265.0
52	125738.5
53	132212.0
54	132212.0
55	121885.0
56	111558.0
57	106974.5
58	102391.0
59	93198.5
60	84006.0
61	84006.0
62	77474.5
63	70943.0
64	62716.5
65	54490.0
66	46832.5
67	39175.0
68	39175.0
69	32648.5
70	26122.0
71	22588.5
72	19055.0
73	15263.5
74	11472.0
75	11472.0
76	8873.5
77	6275.0
78	5178.0
79	4081.0
80	3068.5
81	2056.0
82	2056.0
83	1509.0
84	962.0
85	774.5
86	587.0
87	443.0
88	299.0
89	299.0
90	259.0
91	219.0
92	127.5
93	36.0
94	23.5
95	11.0
96	11.0
97	7.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1462133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.65234123887693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84120947581208	51.20629120009057
2	8.687533461781483	10.364634211727013
3	2.209402057242589	3.9538801635753478
4	0.929166205282133	2.2170775818048867
5	0.4907472866186253	1.4637112301713588
6	0.3223782710484697	1.1538371179549485
7	0.22774932966654593	0.9510046511135991
8	0.16335773080399038	0.7795736881542584
9	0.1290132302432914	0.6926347111322353
>10	0.8074784295232029	9.257952060539363
>50	0.09563363286644586	4.067453127037145
>100	0.08920383343504318	10.494488679549207
>500	0.005977530567150642	2.3950780681932167
>1k	0.0011495251090674313	1.0023835089569526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2481	0.16968360607413963	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1972	0.13487145150270188	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1510	0.10327377878756583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1473	0.10074322924111556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.3678646197028588E-4	0.0	0.0	0.0	0.0
6	1.3678646197028588E-4	0.0	0.0	0.0	0.0
7	1.3678646197028588E-4	0.0	0.0	0.0	0.0
8	1.3678646197028588E-4	0.0	0.0	0.0	0.0
9	1.3678646197028588E-4	0.0	0.0	0.0	0.0
10	1.3678646197028588E-4	0.0	0.0	6.839323098514294E-5	0.0
11	1.3678646197028588E-4	0.0	0.0	6.839323098514294E-5	0.0
12	1.3678646197028588E-4	0.0	0.0	6.839323098514294E-5	0.0
13	1.3678646197028588E-4	0.0	0.0	6.839323098514294E-5	0.0
14	1.3678646197028588E-4	0.0	0.0	1.3678646197028588E-4	0.0
15	1.3678646197028588E-4	0.0	0.0	2.7357292394057176E-4	0.0
16	1.3678646197028588E-4	0.0	0.0	5.471458478811435E-4	0.0
17	1.3678646197028588E-4	0.0	0.0	9.575052337920012E-4	6.839323098514294E-5
18	1.3678646197028588E-4	0.0	0.0	0.0011626849267474299	6.839323098514294E-5
19	1.3678646197028588E-4	0.0	0.0	0.0019150104675840023	6.839323098514294E-5
20	1.3678646197028588E-4	0.0	0.0	0.002188583391524574	6.839323098514294E-5
21	1.3678646197028588E-4	0.0	0.0	0.0028041224703908604	6.839323098514294E-5
22	1.3678646197028588E-4	0.0	0.0	0.004103593859108576	6.839323098514294E-5
23	1.3678646197028588E-4	0.0	0.0	0.005881817864722293	6.839323098514294E-5
24	1.3678646197028588E-4	0.0	0.0	0.008480760642157725	6.839323098514294E-5
25	1.3678646197028588E-4	0.0	0.0	0.009438265875949725	6.839323098514294E-5
26	1.3678646197028588E-4	0.0	0.0	0.0116268492674743	6.839323098514294E-5
27	1.3678646197028588E-4	0.0	0.0	0.016687948360374877	6.839323098514294E-5
28	1.3678646197028588E-4	0.0	0.0	0.030161414864448035	6.839323098514294E-5
29	1.3678646197028588E-4	0.0	0.0	0.052662787858560066	6.839323098514294E-5
30	1.3678646197028588E-4	0.0	0.0	0.08364492149482981	6.839323098514294E-5
31	1.3678646197028588E-4	0.0	0.0	0.18582440858663338	6.839323098514294E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	995	0.0	20.080402	1
CTTACTC	80	1.6171605E-5	18.5	3
CGGCCTT	360	0.0	17.986113	24
TTACTCG	75	2.0677516E-4	17.266666	19
GACGGAC	150	2.5102054E-10	17.266666	7
TAAGAGT	130	1.3938916E-8	17.076923	4
CTTATAC	340	0.0	15.779413	37
TCAATAC	95	7.0617745E-5	15.578948	3
TCTGTCG	155	7.212293E-9	15.5161295	8
ATCTCGC	375	0.0	15.293332	11
TTGGCCG	410	0.0	14.890244	31
ACGTTAC	75	0.004105117	14.799999	16
GACGTTA	75	0.004105117	14.799999	15
GTTATAC	75	0.004105117	14.799999	3
TCTCGCC	400	0.0	14.337501	12
TAGACTA	105	1.6561174E-4	14.095238	5
CGACGTT	80	0.0062997015	13.875001	14
CGTCTAA	80	0.0062997015	13.875001	1
CGTTACT	80	0.0062997015	13.875001	17
ACGGACC	200	9.840733E-10	13.875001	8
>>END_MODULE