FastQCFastQC Report
Fri 10 Feb 2017
ERR1630283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678092
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC82120.4893653029750455No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT50770.3025459867516203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33450.19933352879341537No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT33340.1986780224207016No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG32840.19569844799927535No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG31770.18932215873742322No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG30480.1816348567301435No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT30380.18103894184585828No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG28920.17233858453529366No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT28570.1702528824402953No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG25060.1493362700018831No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG24710.14725056790688473No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT24680.14707179344159915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.14349630413588765No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC23660.14099346162188964No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG23490.1399804063186047No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG23250.1385502105963201No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT23040.1372987893393211No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT22090.13163759793861124No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC21020.12526130867675908No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG20850.12424825337347416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20600.12275846616276104No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT20060.1195405257876207No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG19930.11876583643804987No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA18490.11018466210434232No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA17500.10428510474991837No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT16860.10047124949049277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.021.849141
TCGTTTA1500.020.96666530
CAGGACA24250.018.0804124
TGCATCA22100.017.91402614
GCATCAG22400.017.83928715
GCTGCAT22450.017.5523412
CGAACGA852.7243244E-517.41176416
GACGGAC2450.017.3673467
CTTATAC6100.017.28688437
TTGCGCG650.001580343617.07692318
CCAGGAC25250.016.998023
GACAGGC23950.016.9164927
TCCAGGA25650.016.8771932
ACAGGCT24150.016.6997958
ATCAAGC23850.016.67714930
GGACAGG25800.016.6356586
AGGCCAT24250.016.55463825
GGCCATC24300.016.44444526
ATTTGCG904.4479166E-516.44444516
CTGCATC24500.016.23469413
Produced by FastQC (version 0.11.5)