FastQCFastQC Report
Fri 10 Feb 2017
ERR1630282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803360
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT21350.11839011622748648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.019.612021
ACGAACG2450.018.8775515
TAATACG609.2395936E-418.54
AAGACGG5800.017.5431045
CGAACGA2650.017.45283116
CGAATTA752.0680878E-417.26666615
CAAGACG6000.017.2666664
GGATACG650.001580405417.0769231
TAACGAA2850.016.2280713
ATAACGA2900.015.94827612
AACGAAC2950.015.67796614
CGAAATA750.004105609414.824
TATCTCG750.004105609414.85
GCGAAAG6300.014.68253918
GTATCAA12000.014.6458332
TCTTATA7300.014.44520637
CGAAAGC6550.014.4045819
CGGTCGA908.277235E-414.38888820
GACGGAC6600.014.2954547
CGTTATT4150.014.265062
Produced by FastQC (version 0.11.5)