##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1803360
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83521093957945	33.0	31.0	34.0	30.0	34.0
2	32.04587270428534	33.0	31.0	34.0	30.0	34.0
3	32.17535045692485	34.0	31.0	34.0	30.0	34.0
4	35.814553943749445	37.0	35.0	37.0	35.0	37.0
5	35.74676160056783	37.0	35.0	37.0	35.0	37.0
6	35.798449560819805	37.0	35.0	37.0	35.0	37.0
7	35.763917354272024	37.0	35.0	37.0	35.0	37.0
8	35.76172034424629	37.0	35.0	37.0	35.0	37.0
9	37.51576778901606	39.0	37.0	39.0	35.0	39.0
10	37.39916156507852	39.0	37.0	39.0	34.0	39.0
11	37.47152981101943	39.0	37.0	39.0	35.0	39.0
12	37.376432880844646	39.0	37.0	39.0	34.0	39.0
13	37.3921984517789	39.0	37.0	39.0	34.0	39.0
14	38.623939202377784	40.0	38.0	41.0	34.0	41.0
15	38.63014373170083	40.0	38.0	41.0	34.0	41.0
16	38.58724658415402	40.0	38.0	41.0	34.0	41.0
17	38.54305851299796	40.0	38.0	41.0	34.0	41.0
18	38.4901799973383	40.0	38.0	41.0	34.0	41.0
19	38.50802834708544	40.0	38.0	41.0	34.0	41.0
20	38.48457878626564	40.0	38.0	41.0	34.0	41.0
21	38.425244543518765	40.0	38.0	41.0	34.0	41.0
22	38.38835451601455	40.0	38.0	41.0	34.0	41.0
23	38.32094201934167	40.0	38.0	41.0	34.0	41.0
24	38.2784934788395	40.0	38.0	41.0	34.0	41.0
25	38.255409346996714	40.0	38.0	41.0	34.0	41.0
26	38.0927058379913	40.0	38.0	41.0	33.0	41.0
27	37.96934056428001	40.0	37.0	41.0	33.0	41.0
28	37.87516801969657	40.0	37.0	41.0	33.0	41.0
29	37.81447132020229	40.0	37.0	41.0	33.0	41.0
30	37.74744587880401	40.0	37.0	41.0	32.0	41.0
31	37.705448163428265	40.0	37.0	41.0	32.0	41.0
32	37.624763219767544	40.0	37.0	41.0	32.0	41.0
33	37.51876885369533	40.0	37.0	41.0	32.0	41.0
34	37.457292498447345	40.0	37.0	41.0	31.0	41.0
35	37.328204019164225	40.0	36.0	41.0	31.0	41.0
36	37.237684100789636	39.0	36.0	41.0	31.0	41.0
37	37.178134149587436	39.0	36.0	41.0	31.0	41.0
38	37.0642417487357	39.0	36.0	41.0	31.0	41.0
39	36.989904955194746	39.0	36.0	41.0	31.0	41.0
40	36.87619332800994	39.0	35.0	41.0	30.0	41.0
41	36.76157395084731	39.0	35.0	41.0	30.0	41.0
42	36.7094318383462	39.0	35.0	41.0	30.0	41.0
43	35.415099591872945	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	2.0
13	1.0
14	5.0
15	9.0
16	18.0
17	37.0
18	67.0
19	182.0
20	364.0
21	637.0
22	1172.0
23	2025.0
24	3215.0
25	5256.0
26	7537.0
27	11061.0
28	15617.0
29	21458.0
30	28730.0
31	37924.0
32	48456.0
33	62758.0
34	83517.0
35	110639.0
36	153559.0
37	229808.0
38	421942.0
39	557356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.602808091562416	18.718225978174075	11.819437050838436	26.85952887942507
2	19.53220654777748	20.34075281696389	33.12084109661964	27.006199538638988
3	19.981922633306716	21.93544272912785	29.25771892467394	28.824915712891492
4	14.72551237689646	15.107299707213201	34.240196078431374	35.926991837458964
5	15.61097063259693	35.22142001597019	33.66388297400408	15.5037263774288
6	35.98715730636146	34.682869754236535	15.043751663561352	14.286221275840653
7	30.456481235027947	29.709819448141246	20.608974358974358	19.224724957856445
8	27.99590763907373	32.38044539082601	19.438769851832134	20.184877118268123
9	27.06248336438648	13.587470055895661	18.953675361547333	40.39637121817052
10	17.491183124833647	25.30831337059711	32.41820823351965	24.782295271049595
11	37.84934788394996	20.565111791322863	20.73224425516813	20.853296069559047
12	22.25501286487446	24.2517855558513	28.666821932392867	24.826379646881378
13	30.808768077366693	18.413516990506608	23.931106379203264	26.846608552923435
14	23.285866382752197	19.914104782184367	23.736913317363147	33.06311551770029
15	26.120075858397655	27.104072398190045	20.99148256587703	25.784369177535265
16	26.41702155975512	25.295725756365893	22.73239952089433	25.554853162984653
17	24.82998402981102	25.30066098837725	24.239087037529945	25.630267944281787
18	25.430086061573952	23.861236802413273	25.25230680507497	25.456370330937805
19	25.408515216041167	24.44514683701535	24.712536598349747	25.433801348593736
20	25.87109617602697	23.999811463046758	24.42218969035578	25.706902670570493
21	26.628016591251885	24.278125277260227	24.013397214089256	25.080460917398632
22	26.076324194836303	24.073451778901607	24.456625410345133	25.393598615916957
23	25.391269630023956	23.851976310886343	24.55627273533848	26.20048132375122
24	25.54936341052258	24.55549640670748	24.461449738266346	25.433690444503593
25	26.004347440333596	23.95467349835862	24.28228418064058	25.7586948806672
26	25.600268387898144	24.902848017034867	24.383816875166357	25.11306671990063
27	26.233364386478573	24.012620885458254	24.123303167420815	25.630711560642354
28	25.279755567385326	24.258217993079583	24.926082423919794	25.535944015615296
29	24.837192795670305	24.61710362878183	25.07718924673942	25.468514328808446
30	24.839133617247803	25.0944902848017	24.779633572886166	25.286742525064327
31	25.74632907461627	24.767766835240884	23.94996007452755	25.535944015615296
32	25.270661431993613	24.267533936651585	24.096741637831602	26.365062993523203
33	24.839965397923873	24.08337769496939	24.90955771448851	26.167099192618227
34	25.866937272646616	23.89955416555763	24.68342427468725	25.55008428710851
35	25.835163250820692	24.104893088457104	25.13918463312927	24.920759027592936
36	24.845732410611305	24.991959453464645	24.618434477863545	25.543873658060512
37	25.68777171502085	24.07195457368468	24.85260846420016	25.387665247094315
38	24.89380933368823	24.066076656907107	25.59178422500222	25.44832978440245
39	25.218924673941974	23.790757253127495	25.212270428533408	25.778047644397123
40	24.96323529411765	23.87537707390649	25.88107754413983	25.28031008783604
41	24.260436074882442	24.12036420903203	26.272014461893356	25.347185254192173
42	25.339366515837103	23.916411587259336	26.085640138408305	24.658581758495256
43	24.43383461982078	23.06034291544672	26.36761378759649	26.13820867713601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	44.5
2	52.0
3	144.5
4	237.0
5	237.0
6	292.5
7	348.0
8	325.0
9	302.0
10	458.5
11	615.0
12	615.0
13	1112.5
14	1610.0
15	2483.0
16	3356.0
17	3693.5
18	4031.0
19	4031.0
20	5706.5
21	7382.0
22	8530.5
23	9679.0
24	12469.0
25	15259.0
26	15259.0
27	18490.5
28	21722.0
29	25862.0
30	30002.0
31	35800.5
32	41599.0
33	41599.0
34	48475.5
35	55352.0
36	64662.5
37	73973.0
38	81665.5
39	89358.0
40	89358.0
41	94716.0
42	100074.0
43	103566.5
44	107059.0
45	111906.0
46	116753.0
47	116753.0
48	121941.5
49	127130.0
50	127349.0
51	127568.0
52	129876.0
53	132184.0
54	132184.0
55	132429.5
56	132675.0
57	130343.0
58	128011.0
59	124119.5
60	120228.0
61	120228.0
62	111107.0
63	101986.0
64	91140.0
65	80294.0
66	69724.0
67	59154.0
68	59154.0
69	50924.5
70	42695.0
71	36595.0
72	30495.0
73	23893.5
74	17292.0
75	17292.0
76	13374.5
77	9457.0
78	7683.5
79	5910.0
80	4961.5
81	4013.0
82	4013.0
83	2986.0
84	1959.0
85	1703.5
86	1448.0
87	1264.0
88	1080.0
89	1080.0
90	948.0
91	816.0
92	463.0
93	110.0
94	71.5
95	33.0
96	33.0
97	23.5
98	14.0
99	11.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1803360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.17491851464786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1613717598049	45.83219898389239
2	12.040603184448345	13.768410118760874
3	3.513453620601888	6.026442733887222
4	1.4352317236740808	3.282370274028127
5	0.7367952256981954	2.1063103495637954
6	0.4478184571566728	1.5362390278372855
7	0.3013584285672567	1.2061100517924659
8	0.23055191349023227	1.0545429493760137
9	0.14830283726601853	0.7631282372558122
>10	0.839391756075186	8.759814095768128
>50	0.06825186795122234	2.7520349390724355
>100	0.06705479724694777	8.244831908178615
>500	0.007579459262215489	2.9446469919468807
>1k	0.0022349687568071317	1.7229193386401147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2135	0.11839011622748648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.545204507142223E-5	0.0
10	0.0	0.0	0.0	5.545204507142223E-5	0.0
11	0.0	0.0	0.0	5.545204507142223E-5	0.0
12	0.0	0.0	0.0	5.545204507142223E-5	0.0
13	0.0	0.0	0.0	5.545204507142223E-5	0.0
14	0.0	5.545204507142223E-5	0.0	5.545204507142223E-5	0.0
15	0.0	5.545204507142223E-5	0.0	5.545204507142223E-5	0.0
16	0.0	5.545204507142223E-5	0.0	1.663561352142667E-4	0.0
17	0.0	5.545204507142223E-5	0.0	1.663561352142667E-4	0.0
18	0.0	5.545204507142223E-5	0.0	1.663561352142667E-4	0.0
19	0.0	5.545204507142223E-5	0.0	1.663561352142667E-4	0.0
20	0.0	5.545204507142223E-5	0.0	2.2180818028568893E-4	0.0
21	0.0	5.545204507142223E-5	0.0	2.2180818028568893E-4	0.0
22	0.0	5.545204507142223E-5	0.0	5.545204507142223E-4	0.0
23	0.0	5.545204507142223E-5	0.0	7.208765859284891E-4	0.0
24	0.0	5.545204507142223E-5	0.0	9.981368112856003E-4	0.0
25	0.0	5.545204507142223E-5	0.0	0.0012753970366427114	0.0
26	0.0	5.545204507142223E-5	0.0	0.0025507940732854228	0.0
27	0.0	5.545204507142223E-5	0.0	0.007375121994499157	0.0
28	0.0	5.545204507142223E-5	0.0	0.021959009848283206	0.0
29	0.0	5.545204507142223E-5	0.0	0.0431416910655665	0.0
30	0.0	5.545204507142223E-5	0.0	0.07297489131399167	0.0
31	0.0	5.545204507142223E-5	0.0	0.1715686274509804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	915	0.0	19.61202	1
ACGAACG	245	0.0	18.87755	15
TAATACG	60	9.2395936E-4	18.5	4
AAGACGG	580	0.0	17.543104	5
CGAACGA	265	0.0	17.452831	16
CGAATTA	75	2.0680878E-4	17.266666	15
CAAGACG	600	0.0	17.266666	4
GGATACG	65	0.0015804054	17.076923	1
TAACGAA	285	0.0	16.22807	13
ATAACGA	290	0.0	15.948276	12
AACGAAC	295	0.0	15.677966	14
CGAAATA	75	0.0041056094	14.8	24
TATCTCG	75	0.0041056094	14.8	5
GCGAAAG	630	0.0	14.682539	18
GTATCAA	1200	0.0	14.645833	2
TCTTATA	730	0.0	14.445206	37
CGAAAGC	655	0.0	14.40458	19
CGGTCGA	90	8.277235E-4	14.388888	20
GACGGAC	660	0.0	14.295454	7
CGTTATT	415	0.0	14.26506	2
>>END_MODULE