FastQCFastQC Report
Fri 10 Feb 2017
ERR1630281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97095
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27152.7962304959060713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25982.6757299551985168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16561.7055461146300015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11611.195736134713425No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6460.6653277717699161No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4470.4603738606519388No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.4418353159276997No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.4253566095061538No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.3172150986147588No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.28425768577166693No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2730.28116792831762705No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.2657191410474278No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2310.23791132396106907No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2180.22452237499356298No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2170.22349245584221636No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1810.1864153663937381No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.1709665791235388No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.16478706421545908No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA1420.14624851949121995No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1400.1441886811885267No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1380.14212884288583347No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.14212884288583347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1370.14109892373448685No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1350.1390390854317936No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1330.13697924712910037No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1300.1338894896750605No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA1280.13182965137236727No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1270.13079973222102065No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA1210.12462021731294093No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA1160.11947062155620784No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.11844070240486122No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.11638086410216798No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1090.11226118749678149No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1070.11020134919408825No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1070.11020134919408825No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1060.10917143004274164No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA1050.10814151089139502No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1020.10505175343735516No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1010.10402183428600854No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA980.1009320768319687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCA200.001833646137.05
CATTCCC200.001833646137.010
CCACACT200.001833646137.025
TAGAAGT250.00547188829.631
ACGCCTA250.00547188829.66
CAATTCG250.00547188829.615
CCGTATA250.00547188829.62
ATACCAT250.00547188829.66
TACCATT250.00547188829.67
TTTGCGT451.3110577E-424.6666663
TTGCGTT400.001918544223.1254
TCAGTGT603.681244E-521.5833328
GAATCAG656.819288E-519.9230775
ATCAGTG701.20466764E-418.57
TGTGTTA752.0428051E-417.26666616
AGCCCCG650.001565888217.07692313
GAAACAT650.001565888217.07692327
GCGAACC650.001565888217.07692311
ACATCTA650.001565888217.07692330
GGTTAAT650.001565888217.0769231
Produced by FastQC (version 0.11.5)