FastQCFastQC Report
Fri 10 Feb 2017
ERR1630280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780826
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.30547622283142767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46460.26089017119022295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40470.22725409444830655No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG25390.142574288560477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.1214043370885196No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19800.11118436051585051No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18470.10371591609736157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT555.144619E-420.18181810
ACGTTAA1303.274181E-1119.92307720
GGTATCA17550.019.7122521
TCTAGCG2150.018.06976928
AAGACGG2800.017.8392875
GACGGAC2700.017.8148147
TACGTTA1351.1532393E-917.81481419
TTGAACG852.724476E-517.41176410
AACGCTA1502.5102054E-1017.26666633
AACCGCG1401.87174E-917.1785727
AATTACG1401.87174E-917.17857216
ATTAGAC1005.8814385E-616.6500023
CTTAACG1452.9831426E-916.58620630
ATTACGT1452.9831426E-916.58620617
TTACGTT1606.2937033E-1016.187518
TAACGCT1504.6748028E-916.03333332
CTAGCGG2600.015.65384629
ACGCTTT957.063157E-515.57894814
ACGGACC2850.015.5789478
TCTTATA11350.015.32158737
Produced by FastQC (version 0.11.5)