FastQCFastQC Report
Fri 10 Feb 2017
ERR1630279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549011
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.2567444646939241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33120.21381384638327294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28370.18314911901852216No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG22900.1478362645584828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC806.963328E-720.81253
AGTCGGT2650.020.24528311
TCGGTGA2950.018.81355913
GGTATCA15050.018.807311
GTAGACG500.007035959518.54
GTCGGTG3000.018.512
TATACAG1950.018.0256425
TACGTTA953.6080255E-617.52631619
GTCTTAG1800.017.4722231
GCAGTCG3400.017.4117649
CGGTGAT3100.017.30645214
AGTACTA1401.869921E-917.1785725
CAGTCGG3350.017.11940410
TAATTCG650.001580269417.0769235
TACCGTC904.4475604E-516.4444457
TAGGACA6950.016.237414
GCTTAGG6350.016.0236231
CGCCTTA2350.015.74468125
GTATAGA1659.749783E-1015.696971
CTTAACG957.06221E-515.57894830
Produced by FastQC (version 0.11.5)