##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1549011
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843713182153	33.0	31.0	34.0	30.0	34.0
2	32.05016362052949	33.0	31.0	34.0	30.0	34.0
3	32.18377919846922	34.0	31.0	34.0	30.0	34.0
4	35.82431047939621	37.0	35.0	37.0	35.0	37.0
5	35.756586622044644	37.0	35.0	37.0	35.0	37.0
6	35.81634991617232	37.0	35.0	37.0	35.0	37.0
7	35.79521836836536	37.0	35.0	37.0	35.0	37.0
8	35.791867197844304	37.0	35.0	37.0	35.0	37.0
9	37.541541021981125	39.0	37.0	39.0	35.0	39.0
10	37.42358382219364	39.0	37.0	39.0	34.0	39.0
11	37.49837476944967	39.0	37.0	39.0	35.0	39.0
12	37.41941600156487	39.0	37.0	39.0	34.0	39.0
13	37.420914376979894	39.0	37.0	39.0	34.0	39.0
14	38.66318960937011	40.0	38.0	41.0	34.0	41.0
15	38.682561324612934	40.0	38.0	41.0	35.0	41.0
16	38.635375733290466	40.0	38.0	41.0	34.0	41.0
17	38.60947469062518	40.0	38.0	41.0	34.0	41.0
18	38.56064611548917	40.0	38.0	41.0	34.0	41.0
19	38.601392759638244	40.0	38.0	41.0	34.0	41.0
20	38.58433736106458	40.0	38.0	41.0	34.0	41.0
21	38.53151139662662	40.0	38.0	41.0	34.0	41.0
22	38.50370074841302	40.0	38.0	41.0	34.0	41.0
23	38.45555131629149	40.0	38.0	41.0	34.0	41.0
24	38.42948823475108	40.0	38.0	41.0	34.0	41.0
25	38.40304942960379	40.0	38.0	41.0	34.0	41.0
26	38.2542622357104	40.0	38.0	41.0	34.0	41.0
27	38.138030653107045	40.0	38.0	41.0	33.0	41.0
28	38.08500197868188	40.0	38.0	41.0	33.0	41.0
29	38.04044193359505	40.0	38.0	41.0	33.0	41.0
30	37.97297888781939	40.0	37.0	41.0	33.0	41.0
31	37.917582250868456	40.0	37.0	41.0	33.0	41.0
32	37.82786113203844	40.0	37.0	41.0	33.0	41.0
33	37.753602137105545	40.0	37.0	41.0	33.0	41.0
34	37.6995999382832	40.0	37.0	41.0	33.0	41.0
35	37.58349811589459	40.0	37.0	41.0	32.0	41.0
36	37.50637535821243	40.0	37.0	41.0	32.0	41.0
37	37.45314268265364	40.0	37.0	41.0	32.0	41.0
38	37.34684388942364	40.0	37.0	41.0	31.0	41.0
39	37.266382872684574	40.0	36.0	41.0	31.0	41.0
40	37.18762681478698	39.0	36.0	41.0	31.0	41.0
41	37.0777483181204	39.0	36.0	41.0	31.0	41.0
42	37.04911972865267	39.0	36.0	41.0	31.0	41.0
43	35.82050030632449	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	2.0
11	4.0
12	3.0
13	3.0
14	0.0
15	2.0
16	4.0
17	19.0
18	64.0
19	125.0
20	191.0
21	409.0
22	740.0
23	1318.0
24	2257.0
25	3525.0
26	5398.0
27	8051.0
28	11823.0
29	16790.0
30	22892.0
31	29960.0
32	39527.0
33	51638.0
34	67764.0
35	91537.0
36	127734.0
37	193884.0
38	367283.0
39	506059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7758098554497	18.584826059982788	12.25388328423749	26.38548080033002
2	17.39219411611667	21.076028511095142	35.61646753961076	25.91530983317743
3	19.179915442821258	22.785119021104432	30.700621235097746	27.33434430097656
4	14.148188747529874	15.49433800018205	35.27850996539082	35.07896328689725
5	13.903322829857245	37.30606173874814	34.777351484269644	14.01326394712497
6	34.21964079015578	35.73299350359681	16.020867508365015	14.02649819788239
7	29.38655697086722	31.267111724836038	20.901852859663357	18.444478444633383
8	27.47637040666593	34.7279651338822	19.885849745418206	17.909814714033665
9	26.95803967822049	14.392150862711755	18.83801987203448	39.811789587033275
10	16.29078166649559	27.49903002625546	33.3597372775274	22.850451029721544
11	35.65913992863834	21.97531198939194	21.493327032538826	20.872221049430895
12	21.33483881005364	25.40027152809115	29.963312074607607	23.301577587247603
13	30.211986874205543	19.525232551608738	25.44404139157178	24.81873918261394
14	22.667818369269167	20.221547813411267	25.28219618840667	31.828437628912898
15	25.022094743032813	27.56565318128793	22.52592137822133	24.886330697457925
16	24.74288433071166	26.924792658025027	23.626042681427055	24.706280329836265
17	23.432112489840293	26.96882075078873	25.49233026750617	24.106736491864808
18	23.310228268230503	25.800268687569034	26.669081110463388	24.220421933737075
19	24.429071194458913	25.808854811231168	26.66017220019742	23.1019017941125
20	24.845207684128777	24.978453994193714	26.5859958386351	23.590342483042406
21	24.478522102167126	25.61389170251212	25.440103395004943	24.467482800315814
22	24.565932714486856	25.31886474660283	25.665924903051046	24.449277635859268
23	23.91099869529655	25.80853202462733	25.875090622339027	24.4053786577371
24	24.521775507081614	25.658759040445805	25.988195048324382	23.831270404148196
25	24.567159303581445	25.218219883525684	25.862953846034664	24.351666966858208
26	24.3547011609343	25.718926463401488	25.7456531941994	24.18071918146482
27	24.56244661916539	25.214862902845752	25.58438900692119	24.63830147106767
28	23.82771975150596	25.4850352902594	26.25275094883122	24.43449400940342
29	23.688534167930374	25.960499957714955	26.184578418100322	24.166387456254345
30	23.565100570622157	26.646679720156925	26.052816926413048	23.735402782807867
31	24.395049486414237	25.929835230350207	25.676447746336212	23.99866753689935
32	23.42417193938584	26.07076386158652	26.03893710244795	24.466127096579687
33	23.773620716702464	25.564892696049284	26.332802026583412	24.328684560664836
34	24.125329000245962	25.365023230951877	26.240485057885323	24.269162710916838
35	24.256896819970937	25.58768143028035	26.387675749236127	23.767746000512584
36	23.757287714548188	26.06469547343434	26.19335821372476	23.984658598292715
37	24.63274954148163	25.211828708769662	25.86947413543222	24.285947614316488
38	24.116032746055385	25.592781458621015	26.456106509250095	23.8350792860735
39	24.107769408997097	25.42151088662379	26.351717321568408	24.11900238281071
40	23.622040127539442	25.06786588345725	26.96146121622119	24.348632772782118
41	23.113457554529955	25.382905608804585	27.315235334029257	24.188401502636196
42	23.849604683246277	24.823193637746925	27.00632855415488	24.32087312485192
43	23.353869017069602	24.659540829600306	27.27482245122856	24.711767702101533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	81.0
2	84.0
3	246.5
4	409.0
5	409.0
6	534.5
7	660.0
8	650.5
9	641.0
10	997.0
11	1353.0
12	1353.0
13	2311.5
14	3270.0
15	6067.5
16	8865.0
17	8952.0
18	9039.0
19	9039.0
20	10759.5
21	12480.0
22	13633.5
23	14787.0
24	17315.5
25	19844.0
26	19844.0
27	23262.5
28	26681.0
29	33324.5
30	39968.0
31	44399.5
32	48831.0
33	48831.0
34	54068.5
35	59306.0
36	63830.0
37	68354.0
38	75897.5
39	83441.0
40	83441.0
41	88676.0
42	93911.0
43	91703.5
44	89496.0
45	95444.0
46	101392.0
47	101392.0
48	106022.0
49	110652.0
50	114575.5
51	118499.0
52	123199.0
53	127899.0
54	127899.0
55	117945.5
56	107992.0
57	102663.0
58	97334.0
59	88224.0
60	79114.0
61	79114.0
62	73191.0
63	67268.0
64	59089.5
65	50911.0
66	43754.5
67	36598.0
68	36598.0
69	31084.5
70	25571.0
71	21932.5
72	18294.0
73	14720.0
74	11146.0
75	11146.0
76	8876.0
77	6606.0
78	5405.5
79	4205.0
80	3066.5
81	1928.0
82	1928.0
83	1459.0
84	990.0
85	743.5
86	497.0
87	436.0
88	375.0
89	375.0
90	278.5
91	182.0
92	112.5
93	43.0
94	29.0
95	15.0
96	15.0
97	8.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1549011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.80791827271694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93259713825124	50.198339100614064
2	10.011530120259437	11.97537550434641
3	2.681809907850237	4.811804033750087
4	1.0527780188474802	2.518578468421718
5	0.5527195173780552	1.6528501861541138
6	0.3449619488174132	1.2378873625241407
7	0.23255879532431228	0.9736200197050584
8	0.16425512881953214	0.7859005856250536
9	0.12457045394633347	0.6705269575935806
>10	0.7334249612975383	8.185606916289375
>50	0.08147553705421252	3.451579481437629
>100	0.07953775274654412	9.395552991586557
>500	0.006159736197758671	2.492717497048228
>1k	0.0016209832099364923	1.649660894904039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3977	0.2567444646939241	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3312	0.21381384638327294	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2837	0.18314911901852216	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2290	0.1478362645584828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.455732076789641E-5	0.0
5	0.0	0.0	0.0	6.455732076789641E-5	0.0
6	0.0	0.0	0.0	6.455732076789641E-5	0.0
7	0.0	0.0	0.0	6.455732076789641E-5	0.0
8	0.0	0.0	0.0	6.455732076789641E-5	0.0
9	0.0	0.0	0.0	6.455732076789641E-5	0.0
10	0.0	0.0	0.0	6.455732076789641E-5	0.0
11	0.0	0.0	0.0	1.2911464153579283E-4	0.0
12	0.0	0.0	0.0	1.2911464153579283E-4	0.0
13	0.0	0.0	0.0	1.2911464153579283E-4	0.0
14	0.0	0.0	0.0	1.9367196230368926E-4	0.0
15	0.0	0.0	0.0	1.9367196230368926E-4	0.0
16	0.0	6.455732076789641E-5	0.0	2.5822928307158566E-4	0.0
17	0.0	6.455732076789641E-5	0.0	2.5822928307158566E-4	0.0
18	0.0	1.2911464153579283E-4	0.0	2.5822928307158566E-4	0.0
19	0.0	1.2911464153579283E-4	0.0	2.5822928307158566E-4	0.0
20	0.0	1.2911464153579283E-4	0.0	2.5822928307158566E-4	0.0
21	0.0	1.2911464153579283E-4	0.0	3.873439246073785E-4	0.0
22	6.455732076789641E-5	1.2911464153579283E-4	0.0	7.74687849214757E-4	0.0
23	6.455732076789641E-5	1.2911464153579283E-4	0.0	0.0016139330191974105	0.0
24	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.002194948906108478	0.0
25	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.002453178189180064	0.0
26	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.0030341940760911317	0.0
27	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.007165862605236503	0.0
28	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.019883654796512096	0.0
29	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.038992621743809436	0.0
30	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.06681682699477279	0.0
31	6.455732076789641E-5	1.9367196230368926E-4	0.0	0.15700340410752409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATAC	80	6.963328E-7	20.8125	3
AGTCGGT	265	0.0	20.245283	11
TCGGTGA	295	0.0	18.813559	13
GGTATCA	1505	0.0	18.80731	1
GTAGACG	50	0.0070359595	18.5	4
GTCGGTG	300	0.0	18.5	12
TATACAG	195	0.0	18.025642	5
TACGTTA	95	3.6080255E-6	17.526316	19
GTCTTAG	180	0.0	17.472223	1
GCAGTCG	340	0.0	17.411764	9
CGGTGAT	310	0.0	17.306452	14
AGTACTA	140	1.869921E-9	17.178572	5
CAGTCGG	335	0.0	17.119404	10
TAATTCG	65	0.0015802694	17.076923	5
TACCGTC	90	4.4475604E-5	16.444445	7
TAGGACA	695	0.0	16.23741	4
GCTTAGG	635	0.0	16.023623	1
CGCCTTA	235	0.0	15.744681	25
GTATAGA	165	9.749783E-10	15.69697	1
CTTAACG	95	7.06221E-5	15.578948	30
>>END_MODULE