FastQCFastQC Report
Fri 10 Feb 2017
ERR1630278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596204
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10030.16823100817840872No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG7090.11891902771534577No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6030.1011398782966904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTT250.005494247729.610
GGTATAT405.9354083E-527.751
GACGGAC751.3709723E-824.6666647
AAGACGG851.9717845E-923.9411775
CGGTTGA400.00193010323.12525
GTACTAG400.00193010323.1251
CTTACTC400.00193010323.1253
CGAACGA855.1701136E-821.76470616
GGTATCA3000.020.9666651
CTTTAGC450.003823681720.5555551
ACGGACC951.6727427E-719.4736848
ACGAGAC951.6727427E-719.47368419
TCTTATA2900.018.50000237
ATTACAC609.2297076E-418.53
AGACGGA1002.8691647E-718.56
AACGAGA1002.8691647E-718.518
TCGAGCT500.007031007718.527
CGCAAGT500.007031007718.516
ACGAACG1002.8691647E-718.515
CGGACCA1156.389564E-817.6956529
Produced by FastQC (version 0.11.5)