##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596204
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7408974109533	31.0	31.0	34.0	30.0	34.0
2	31.95820725791843	33.0	31.0	34.0	30.0	34.0
3	32.07846643095316	33.0	31.0	34.0	30.0	34.0
4	35.73677801557856	37.0	35.0	37.0	33.0	37.0
5	35.66561109955653	37.0	35.0	37.0	33.0	37.0
6	35.724579170887814	37.0	35.0	37.0	33.0	37.0
7	35.697677305083495	37.0	35.0	37.0	33.0	37.0
8	35.70410966716091	37.0	35.0	37.0	33.0	37.0
9	37.447524672763016	39.0	37.0	39.0	35.0	39.0
10	37.322052518936474	39.0	37.0	39.0	34.0	39.0
11	37.394579707616856	39.0	37.0	39.0	34.0	39.0
12	37.31146218408464	39.0	37.0	39.0	34.0	39.0
13	37.31174396683014	39.0	37.0	39.0	34.0	39.0
14	38.52419641599184	40.0	38.0	41.0	34.0	41.0
15	38.53575286311397	40.0	38.0	41.0	34.0	41.0
16	38.48496823235	40.0	38.0	41.0	34.0	41.0
17	38.441496534743145	40.0	38.0	41.0	34.0	41.0
18	38.384609965716436	40.0	38.0	41.0	34.0	41.0
19	38.42159059650724	40.0	38.0	41.0	34.0	41.0
20	38.40161085802846	40.0	38.0	41.0	34.0	41.0
21	38.33156771843195	40.0	38.0	41.0	34.0	41.0
22	38.29085178898498	40.0	38.0	41.0	34.0	41.0
23	38.23303768508765	40.0	38.0	41.0	34.0	41.0
24	38.18280152431047	40.0	38.0	41.0	33.0	41.0
25	38.16340883321816	40.0	38.0	41.0	33.0	41.0
26	37.98796385129922	40.0	37.0	41.0	33.0	41.0
27	37.86416729844147	40.0	37.0	41.0	33.0	41.0
28	37.7830692179187	40.0	37.0	41.0	32.0	41.0
29	37.72823396018812	40.0	37.0	41.0	32.0	41.0
30	37.668358481325185	40.0	37.0	41.0	32.0	41.0
31	37.625639881651246	40.0	37.0	41.0	32.0	41.0
32	37.538050734312414	40.0	37.0	41.0	32.0	41.0
33	37.44880443606551	40.0	37.0	41.0	31.0	41.0
34	37.40106909715467	40.0	37.0	41.0	31.0	41.0
35	37.257505820155515	39.0	36.0	41.0	31.0	41.0
36	37.18129197388813	39.0	36.0	41.0	31.0	41.0
37	37.13051405223715	39.0	36.0	41.0	31.0	41.0
38	37.01288317421554	39.0	36.0	41.0	30.0	41.0
39	36.94461291772615	39.0	36.0	41.0	30.0	41.0
40	36.84298830601606	39.0	36.0	41.0	30.0	41.0
41	36.73000348873875	39.0	35.0	41.0	30.0	41.0
42	36.69804295174135	39.0	35.0	41.0	30.0	41.0
43	35.36936518372906	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	3.0
16	3.0
17	12.0
18	31.0
19	69.0
20	137.0
21	249.0
22	402.0
23	690.0
24	1102.0
25	1787.0
26	2635.0
27	3940.0
28	5421.0
29	7441.0
30	9857.0
31	12925.0
32	16875.0
33	21683.0
34	28017.0
35	37291.0
36	51691.0
37	76975.0
38	142846.0
39	174121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01565236060141	19.001885260749678	12.412194483767301	26.570267894881617
2	19.662900617909308	20.822738525739513	33.41725315496038	26.097107701390797
3	20.447363654051298	22.137724671421193	29.375515763060967	28.039395911466546
4	15.496709180079302	15.276314818417857	33.54321675131331	35.68375925018953
5	15.227002837954796	35.99959074410772	33.76444975209827	15.008956665839209
6	36.18073679478836	34.129257770830115	15.031935377823698	14.658070056557824
7	29.91258025776412	29.904864777827722	20.63119334992721	19.55136161448095
8	27.123601988581093	33.30588187935673	19.801946984589165	19.768569147473013
9	26.805086849467628	13.999906072418167	18.835331530818312	40.35967554729589
10	17.289719626168225	26.34148714198496	32.512026085031295	23.856767146815518
11	37.1035752863114	20.994324090412007	20.395200300568263	21.506900322708336
12	22.083213128392295	24.99765181045414	28.129968936806865	24.7891661243467
13	30.985535152397503	18.887159428651938	24.33009506813104	25.797210350819515
14	23.509402821852923	20.461620519151165	23.48743047681666	32.54154618217925
15	26.34752534367431	27.213504102622593	21.16003918121985	25.278931372483243
16	26.455038879309768	25.669066292745434	22.424371523840833	25.45152330410396
17	24.878397327089385	25.58251873519802	23.962435676379226	25.576648261333368
18	24.59980141025555	24.21671105863094	25.501506195865847	25.681981335247666
19	25.46343197965797	24.63804335428813	24.851728602961405	25.046796063092497
20	25.93541807837586	23.98172437622022	24.856928165527236	25.22592937987669
21	26.182145708515876	24.759142843724632	23.725436260072055	25.333275187687438
22	25.984730058838924	24.26870668428927	24.184339588463008	25.5622236684088
23	25.09728213832849	24.620767388343587	24.465786878316816	25.816163595011105
24	25.76416796935277	24.956893949050997	24.363472905247196	24.915465176349034
25	25.948668576527496	24.499332443257675	24.226439272463786	25.32555970775104
26	25.861617835505967	25.03589375448672	23.976860269303796	25.125628140703515
27	26.044273436608943	24.024494971519815	24.155658130438574	25.775573461432664
28	25.15649006044911	24.500338810205903	24.752601458561163	25.590569670783825
29	24.63854653776224	24.867830474133015	24.888125540922236	25.605497447182508
30	24.750924180314122	25.81683450630992	24.528517084756224	24.903724228619733
31	26.012740605564538	24.99144588094008	24.062066004253577	24.9337475092418
32	24.765348773238692	24.668234362734903	24.43576359769475	26.13065326633166
33	24.7579687489517	24.407082139670315	24.72878410745315	26.10616500392483
34	25.49865482284587	24.146265372255137	24.939282527457046	25.41579727744195
35	25.71972009580613	24.17394046333134	25.008554119059916	25.097785321802608
36	24.896176476508042	25.245888991016496	24.634017886495226	25.223916645980232
37	26.195396206667514	23.792024206479663	24.540593488134935	25.471986098717885
38	24.972828092397904	24.28229263809032	25.61405156624243	25.130827703269347
39	25.185674701947658	23.83026615051224	25.432066876438263	25.551992271101838
40	24.550824885441898	23.49799732977303	26.374864979101115	25.57631280568396
41	23.977195724953205	23.868675822369525	26.83947105353201	25.314657399145258
42	24.981885394931936	23.366834170854272	26.52514911003616	25.12613132417763
43	24.020972687201024	23.046809481318476	26.790494528718355	26.141723302762145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	13.0
2	20.0
3	34.5
4	49.0
5	49.0
6	78.5
7	108.0
8	99.0
9	90.0
10	137.5
11	185.0
12	185.0
13	319.0
14	453.0
15	750.5
16	1048.0
17	1172.0
18	1296.0
19	1296.0
20	1635.0
21	1974.0
22	2385.0
23	2796.0
24	3551.0
25	4306.0
26	4306.0
27	5278.0
28	6250.0
29	8525.0
30	10800.0
31	12129.0
32	13458.0
33	13458.0
34	15539.5
35	17621.0
36	20090.0
37	22559.0
38	26100.0
39	29641.0
40	29641.0
41	31918.0
42	34195.0
43	33846.5
44	33498.0
45	36619.0
46	39740.0
47	39740.0
48	42347.5
49	44955.0
50	46749.0
51	48543.0
52	51482.5
53	54422.0
54	54422.0
55	50206.5
56	45991.0
57	44240.5
58	42490.0
59	39429.5
60	36369.0
61	36369.0
62	33652.5
63	30936.0
64	27435.5
65	23935.0
66	20591.0
67	17247.0
68	17247.0
69	14446.5
70	11646.0
71	10068.5
72	8491.0
73	6648.0
74	4805.0
75	4805.0
76	3693.5
77	2582.0
78	2118.0
79	1654.0
80	1287.0
81	920.0
82	920.0
83	673.5
84	427.0
85	357.5
86	288.0
87	257.0
88	226.0
89	226.0
90	185.5
91	145.0
92	86.5
93	28.0
94	18.0
95	8.0
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	596204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.41519733215563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51193049619219	56.764167359982274
2	6.329190120971051	8.027336809482183
3	1.4927863952605793	2.839960314906207
4	0.6631304447407165	1.6821019204077063
5	0.3674063869119392	1.1649574263557472
6	0.24306006313453535	0.924822112034566
7	0.16786631708563632	0.7451692936385484
8	0.13143371615961735	0.6667916037088533
9	0.10202208724549422	0.5822775715420272
>10	0.7670770421269851	10.091475155450365
>50	0.13599271627409798	6.179585811635808
>100	0.0865167505837126	9.65334689019972
>500	0.0013228860945519636	0.5097222186916469
>1k	2.645772189103927E-4	0.16828551196435876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1003	0.16823100817840872	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	709	0.11891902771534577	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	603	0.1011398782966904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6772782470429585E-4	0.0
4	0.0	0.0	0.0	1.6772782470429585E-4	0.0
5	1.6772782470429585E-4	0.0	0.0	1.6772782470429585E-4	0.0
6	1.6772782470429585E-4	0.0	0.0	1.6772782470429585E-4	0.0
7	1.6772782470429585E-4	0.0	0.0	1.6772782470429585E-4	0.0
8	1.6772782470429585E-4	0.0	0.0	1.6772782470429585E-4	0.0
9	1.6772782470429585E-4	0.0	0.0	1.6772782470429585E-4	0.0
10	3.354556494085917E-4	0.0	0.0	3.354556494085917E-4	0.0
11	3.354556494085917E-4	1.6772782470429585E-4	0.0	3.354556494085917E-4	0.0
12	3.354556494085917E-4	1.6772782470429585E-4	0.0	3.354556494085917E-4	0.0
13	3.354556494085917E-4	1.6772782470429585E-4	0.0	3.354556494085917E-4	0.0
14	3.354556494085917E-4	1.6772782470429585E-4	0.0	3.354556494085917E-4	0.0
15	3.354556494085917E-4	1.6772782470429585E-4	0.0	5.031834741128876E-4	0.0
16	3.354556494085917E-4	1.6772782470429585E-4	0.0	8.386391235214793E-4	0.0
17	5.031834741128876E-4	1.6772782470429585E-4	0.0	0.001006366948225775	0.0
18	5.031834741128876E-4	1.6772782470429585E-4	0.0	0.001006366948225775	0.0
19	5.031834741128876E-4	1.6772782470429585E-4	0.0	0.0013418225976343668	0.0
20	5.031834741128876E-4	1.6772782470429585E-4	0.0	0.0015095504223386627	0.0
21	5.031834741128876E-4	1.6772782470429585E-4	0.0	0.002180461721155846	0.0
22	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.0030191008446773253	0.0
23	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.0040254677929031	0.0
24	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.005535018215241763	0.0
25	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.006205929514058946	0.0
26	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.007380024286989017	0.0
27	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.013418225976343668	0.0
28	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.03253919799263339	0.0
29	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.06189156731588517	0.0
30	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.09895941657553455	0.0
31	6.709112988171834E-4	1.6772782470429585E-4	0.0	0.2182138999402889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTT	25	0.0054942477	29.6	10
GGTATAT	40	5.9354083E-5	27.75	1
GACGGAC	75	1.3709723E-8	24.666664	7
AAGACGG	85	1.9717845E-9	23.941177	5
CGGTTGA	40	0.001930103	23.125	25
GTACTAG	40	0.001930103	23.125	1
CTTACTC	40	0.001930103	23.125	3
CGAACGA	85	5.1701136E-8	21.764706	16
GGTATCA	300	0.0	20.966665	1
CTTTAGC	45	0.0038236817	20.555555	1
ACGGACC	95	1.6727427E-7	19.473684	8
ACGAGAC	95	1.6727427E-7	19.473684	19
TCTTATA	290	0.0	18.500002	37
ATTACAC	60	9.2297076E-4	18.5	3
AGACGGA	100	2.8691647E-7	18.5	6
AACGAGA	100	2.8691647E-7	18.5	18
TCGAGCT	50	0.0070310077	18.5	27
CGCAAGT	50	0.0070310077	18.5	16
ACGAACG	100	2.8691647E-7	18.5	15
CGGACCA	115	6.389564E-8	17.695652	9
>>END_MODULE