FastQCFastQC Report
Fri 10 Feb 2017
ERR1630277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70188
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC1970.2806747592181No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA1900.2707015444235482No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA1450.20658802074428675No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG1340.19091582606713398No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT1320.18806633612583348No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG1250.1780931213312817No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG1200.17096939647803042No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC1130.16099618168347865No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC970.1382002621530746No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG930.13250128227047359No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG920.13107653729982335No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG870.12395281244657207No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG830.11825383256397104No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA820.1168290875933208No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA820.1168290875933208No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA800.11397959765202029No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT790.11255485268137004No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA780.1111301077107198No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA760.10828061776941926No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG730.10400638285746851No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG710.10115689291616801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAT450.003790643320.5555555
CTAGGTA500.00697083918.54
GCCTAGG700.002560106315.8571422
GGCCTAG750.004053552614.81
TCTCTTA1203.2144388E-513.87500137
ACCCTTG850.00929012613.0588248
CCTGTCT1951.3920362E-712.33333437
GTCTCTT1901.7596598E-510.71052637
TGTCTCT2402.2706231E-48.47916736
CTGTCTC3851.5519054E-67.688312537
Produced by FastQC (version 0.11.5)