##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70188
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776272297258792	31.0	31.0	34.0	30.0	34.0
2	31.997008035561635	33.0	31.0	34.0	30.0	34.0
3	32.12449421553542	34.0	31.0	34.0	30.0	34.0
4	35.75551376303642	37.0	35.0	37.0	33.0	37.0
5	35.67793639938451	37.0	35.0	37.0	33.0	37.0
6	35.746808571265746	37.0	35.0	37.0	35.0	37.0
7	35.705277255371286	37.0	35.0	37.0	33.0	37.0
8	35.720336809711064	37.0	35.0	37.0	33.0	37.0
9	37.458810622898504	39.0	37.0	39.0	35.0	39.0
10	37.363067760870806	39.0	37.0	39.0	34.0	39.0
11	37.41079671738759	39.0	37.0	39.0	34.0	39.0
12	37.33321935373568	39.0	37.0	39.0	34.0	39.0
13	37.33510001709694	39.0	37.0	39.0	34.0	39.0
14	38.53433635379267	40.0	38.0	41.0	34.0	41.0
15	38.545249900267855	40.0	38.0	41.0	34.0	41.0
16	38.49039721889782	40.0	38.0	41.0	34.0	41.0
17	38.46349803385194	40.0	38.0	41.0	34.0	41.0
18	38.389297315780475	40.0	38.0	41.0	34.0	41.0
19	38.38908360403488	40.0	38.0	41.0	34.0	41.0
20	38.39025189491081	40.0	38.0	41.0	34.0	41.0
21	38.31820254174503	40.0	38.0	41.0	34.0	41.0
22	38.29154556334416	40.0	38.0	41.0	34.0	41.0
23	38.222530916965866	40.0	38.0	41.0	34.0	41.0
24	38.16897475351912	40.0	38.0	41.0	33.0	41.0
25	38.15192055622044	40.0	38.0	41.0	33.0	41.0
26	37.99830455348493	40.0	37.0	41.0	33.0	41.0
27	37.849874622442584	40.0	37.0	41.0	33.0	41.0
28	37.76759560038753	40.0	37.0	41.0	32.0	41.0
29	37.73655040747706	40.0	37.0	41.0	32.0	41.0
30	37.67007180714652	40.0	37.0	41.0	32.0	41.0
31	37.60782469937881	40.0	37.0	41.0	32.0	41.0
32	37.5326551547273	40.0	37.0	41.0	32.0	41.0
33	37.44420698694934	40.0	37.0	41.0	31.0	41.0
34	37.412150225109706	40.0	37.0	41.0	31.0	41.0
35	37.26364905681883	39.0	36.0	41.0	31.0	41.0
36	37.16743602895082	39.0	36.0	41.0	31.0	41.0
37	37.117584202427764	39.0	36.0	41.0	31.0	41.0
38	36.978913774434375	39.0	36.0	41.0	30.0	41.0
39	36.91514218954807	39.0	36.0	41.0	30.0	41.0
40	36.82023992705306	39.0	35.0	41.0	30.0	41.0
41	36.6855872798769	39.0	35.0	40.0	30.0	41.0
42	36.674460021656124	39.0	35.0	40.0	30.0	41.0
43	35.32249102410668	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	6.0
20	19.0
21	22.0
22	53.0
23	77.0
24	136.0
25	201.0
26	298.0
27	437.0
28	643.0
29	850.0
30	1176.0
31	1590.0
32	1939.0
33	2532.0
34	3280.0
35	4473.0
36	6218.0
37	9019.0
38	16961.0
39	20255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15712087536331	17.45882487034821	11.527611557531202	26.85644269675728
2	21.603407989969796	19.65435687012025	29.487946657548296	29.254288482361655
3	22.45825497235995	20.009118367812164	27.615831766113864	29.916794893714027
4	17.18384909101271	13.7530632016869	31.254630421154612	37.80845728614578
5	17.22516669516157	33.649626716817686	33.1851598563857	15.940046731635038
6	40.048156380007974	30.694705647689062	13.42822134837864	15.828916623924316
7	32.51695446515074	27.389297315780475	18.766740753405138	21.327007465663648
8	30.620618909215253	28.88812902490454	18.61001880663361	21.881233259246592
9	27.949222089246025	12.855473870177239	17.58562717273608	41.609676867840655
10	19.56459793696928	24.16509944719895	31.005300051290817	25.265002564540946
11	39.50247905624893	19.456317319199865	18.339317262210063	22.701886362341142
12	25.595543397731806	23.055223115062404	25.23793241009859	26.111301077107196
13	34.206702000341934	17.416082521228702	21.80287228586083	26.574343192568527
14	25.42314925628312	18.661309625577022	21.41391690887331	34.50162420926654
15	29.029178776998915	25.15529720180088	19.171368325069814	26.644155696130394
16	28.100245056134952	23.14640679318402	21.064854391064	27.688493759617028
17	27.531771812845502	22.58933150965977	22.08212230010828	27.796774377386445
18	26.78093121331282	21.888356984099847	23.626545848293155	27.70416595429418
19	28.806918561577476	21.85843733971619	23.343021599133756	25.991622499572575
20	29.638969624437227	21.23012480765943	21.453809768051517	27.677095799851827
21	29.174502764005243	21.874109534393344	22.057901635607227	26.89348606599419
22	29.067646891206472	21.6290533994415	22.683364677722686	26.61993503162934
23	28.050378982162194	21.939647803043254	21.764404171653272	28.245569043141277
24	27.459109819342338	22.58648201971847	23.089416994358007	26.86499116658118
25	28.563287171596286	22.37277027412093	21.835641420185787	27.228301134097
26	28.55188921183108	22.68906365760529	21.492277882259074	27.266769248304552
27	28.3709466005585	21.264318686955036	22.30865675044167	28.056077962044796
28	27.47620675899014	22.334302159913374	22.08639653502023	28.103094546076253
29	27.329458027013164	22.622100643984727	23.26181113580669	26.786630193195414
30	27.98911494842423	22.741779221519348	21.771527896506527	27.497577933549895
31	27.397845785604375	23.186299652362226	22.64774605345643	26.768108508576965
32	26.89348606599419	21.941072548013906	22.821564939875763	28.343876446116145
33	26.181113580669056	23.006781786060294	22.81444121502251	27.99766341824813
34	28.77272468228187	20.96797173305978	22.842936114435517	27.41636747022283
35	27.215478429361145	21.757280446800024	22.78879580555081	28.238445318288026
36	26.25804980908417	23.241864706217587	23.548184874907392	26.951900609790847
37	27.722687638912635	21.40394369407876	23.739100700974525	27.13426796603408
38	27.155639140593834	21.958169487661706	23.219068786687185	27.667122585057275
39	27.085826637031975	21.18453296859862	24.26768108508577	27.46195930928364
40	25.90186356642161	20.697270188636235	25.11113010771072	28.289736137231436
41	24.900267852054483	21.28284037157349	25.78788396876959	28.02900780760244
42	25.57702171311335	20.61748447027982	26.76525901863566	27.040234797971163
43	25.738017894796833	20.603237020573317	25.77648600900439	27.882259075625466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.5
4	1.0
5	1.0
6	2.5
7	4.0
8	4.5
9	5.0
10	6.0
11	7.0
12	7.0
13	8.0
14	9.0
15	24.5
16	40.0
17	42.0
18	44.0
19	44.0
20	67.5
21	91.0
22	112.5
23	134.0
24	174.5
25	215.0
26	215.0
27	260.0
28	305.0
29	412.5
30	520.0
31	649.0
32	778.0
33	778.0
34	971.5
35	1165.0
36	1382.5
37	1600.0
38	1783.5
39	1967.0
40	1967.0
41	2266.5
42	2566.0
43	2710.0
44	2854.0
45	3232.0
46	3610.0
47	3610.0
48	4113.0
49	4616.0
50	4912.5
51	5209.0
52	5585.5
53	5962.0
54	5962.0
55	5866.5
56	5771.0
57	6014.5
58	6258.0
59	6304.0
60	6350.0
61	6350.0
62	6107.5
63	5865.0
64	5395.0
65	4925.0
66	4237.5
67	3550.0
68	3550.0
69	3157.0
70	2764.0
71	2217.5
72	1671.0
73	1137.5
74	604.0
75	604.0
76	453.0
77	302.0
78	240.0
79	178.0
80	155.0
81	132.0
82	132.0
83	92.0
84	52.0
85	43.0
86	34.0
87	23.0
88	12.0
89	12.0
90	12.0
91	12.0
92	7.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	70188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.95549096711689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.38565793071125	67.40041032655155
2	3.8569698863414446	5.627742634068502
3	1.1678318946998398	2.555992477346555
4	0.5565754013201578	1.624209266541289
5	0.37300316369175485	1.3606314469709921
6	0.29684021403741745	1.2993674132330313
7	0.18943092606335196	0.9674018350715222
8	0.13084404171386166	0.7636633042685359
9	0.09178611881420146	0.6026671225850573
>10	0.8514627192125923	12.0804126061435
>50	0.08397453423426943	4.070496381147774
>100	0.015623169159864078	1.6470051860716932
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	197	0.2806747592181	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	190	0.2707015444235482	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	145	0.20658802074428675	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	134	0.19091582606713398	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	132	0.18806633612583348	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	125	0.1780931213312817	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	120	0.17096939647803042	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	113	0.16099618168347865	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	97	0.1382002621530746	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	93	0.13250128227047359	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	92	0.13107653729982335	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	87	0.12395281244657207	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	83	0.11825383256397104	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	82	0.1168290875933208	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	82	0.1168290875933208	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	80	0.11397959765202029	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	79	0.11255485268137004	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	78	0.1111301077107198	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	76	0.10828061776941926	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	73	0.10400638285746851	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	71	0.10115689291616801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0014247449706502537	0.0
17	0.0	0.0	0.0	0.0014247449706502537	0.0
18	0.0	0.0	0.0	0.0014247449706502537	0.0
19	0.0	0.0	0.0	0.0014247449706502537	0.0
20	0.0	0.0	0.0	0.0014247449706502537	0.0
21	0.0	0.0	0.0	0.0014247449706502537	0.0
22	0.0	0.0	0.0	0.0014247449706502537	0.0
23	0.0	0.0	0.0	0.004274234911950761	0.0
24	0.0	0.0	0.0	0.007123724853251268	0.0
25	0.0	0.0	0.0	0.008548469823901521	0.0
26	0.0	0.0	0.0	0.017096939647803042	0.0
27	0.0	0.0	0.0	0.025645409471704565	0.0
28	0.0	0.0	0.0	0.05414030888470964	0.0
29	0.0	0.0	0.0	0.09688265800421725	0.0
30	0.0	0.0	0.0	0.14674873197697613	0.0
31	0.0	0.0	0.0	0.32769134324955834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTAT	45	0.0037906433	20.555555	5
CTAGGTA	50	0.006970839	18.5	4
GCCTAGG	70	0.0025601063	15.857142	2
GGCCTAG	75	0.0040535526	14.8	1
TCTCTTA	120	3.2144388E-5	13.875001	37
ACCCTTG	85	0.009290126	13.058824	8
CCTGTCT	195	1.3920362E-7	12.333334	37
GTCTCTT	190	1.7596598E-5	10.710526	37
TGTCTCT	240	2.2706231E-4	8.479167	36
CTGTCTC	385	1.5519054E-6	7.6883125	37
>>END_MODULE