FastQCFastQC Report
Fri 10 Feb 2017
ERR1630276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137491
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC70580.3302002207260756No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT47820.22372024022557288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.16486619124946023No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT32290.15106496354838453No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG31240.1461526621632559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30330.1418953342961444No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG30180.14119357695541174No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG27820.13015259479455119No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG26490.12393034637338825No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT26250.12280753462821598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25500.11929874792455267No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC23340.10919344221800233No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG22840.10685425108222678No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG22510.10531038493261492No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG22260.10414078936472715No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC22220.10395365407386511No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT21900.10245657174696876No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT21720.10161446293808955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1306.9667294E-1018.528
TGCATCA17800.017.46067414
GGTATCA15650.017.2587871
GCTGCAT18400.016.79076212
CGAACGA904.448834E-516.44444516
GACAGGC19700.016.434017
TCTTATA13350.016.3520637
GCATCAG19100.016.27225115
CGCAAGA3300.016.2575762
CAGGACA22200.015.9166684
CGCGCAA1302.5918052E-715.65384621
CCAGGAC23900.015.4811723
CTGCATC20250.015.43950713
CTTATAC7600.015.33552637
TACGCTA1455.3520125E-815.3103459
CGGACCA3150.015.2698419
ACAGGCT21300.015.1995318
GACGGAC3250.014.8000017
TCCAGGA24800.014.4717732
CGCGAAC908.278118E-414.38888828
Produced by FastQC (version 0.11.5)