FastQCFastQC Report
Fri 10 Feb 2017
ERR1630275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951095
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC86100.44129065986023236No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT44040.22571940371944985No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT30890.15832135288133073No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29640.15191469405641447No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG29300.15017208285603725No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG29050.148890751091054No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG26120.13387354280545027No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT26060.1335660231818543No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT25570.1310546129224871No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT22130.11342348783631756No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG22050.11301346167152292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21740.11142461028294368No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC21500.11019453178855976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.10932322618837115No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT20110.10307032717525287No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC19760.10127646270427632No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG19670.10081518326888234No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT19590.1004051571040877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.022.3790321
CAGGACA18600.021.6827954
GACAGGC17850.020.9355757
CCAGGAC19400.020.5979393
TCCAGGA22000.018.52
ACAGGCT20150.018.4540948
TGCATCA19750.018.26582114
GTACTAG1653.6379788E-1217.9393941
GCATCAG19700.017.93654815
TGCGCTA2900.017.86206810
GCGCTAT2900.017.86206811
TTGTGCG2950.017.5593227
GCTGCAT20500.017.50731812
ATCAGAA20600.017.4223317
CTGCATC20600.017.4223313
GGACAGG22750.017.0769236
ATCAAGC20950.017.0429630
GGACCGT4700.016.9255336
TATACAG1554.0199666E-1016.7096795
CCATCAA22150.016.28668228
Produced by FastQC (version 0.11.5)