##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630275.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1951095
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85618434776369	31.0	31.0	34.0	30.0	34.0
2	32.048552223238744	33.0	31.0	34.0	30.0	34.0
3	32.1819132333382	34.0	31.0	34.0	30.0	34.0
4	35.83233107562676	37.0	35.0	37.0	35.0	37.0
5	35.75800870793068	37.0	35.0	37.0	35.0	37.0
6	35.81419869355413	37.0	35.0	37.0	35.0	37.0
7	35.78816203208967	37.0	35.0	37.0	35.0	37.0
8	35.76667512345632	37.0	35.0	37.0	35.0	37.0
9	37.487233579092766	39.0	37.0	39.0	35.0	39.0
10	37.39898159751319	39.0	37.0	39.0	34.0	39.0
11	37.47522391272593	39.0	37.0	39.0	35.0	39.0
12	37.34430409590512	39.0	37.0	39.0	34.0	39.0
13	37.37256309918277	39.0	37.0	39.0	34.0	39.0
14	38.59312796147804	40.0	38.0	41.0	34.0	41.0
15	38.59641585878699	40.0	38.0	41.0	34.0	41.0
16	38.571377098501095	40.0	38.0	41.0	34.0	41.0
17	38.52632906137323	40.0	38.0	41.0	34.0	41.0
18	38.449138048121696	40.0	38.0	41.0	34.0	41.0
19	38.43969617061189	40.0	38.0	41.0	34.0	41.0
20	38.382139260261546	40.0	38.0	41.0	34.0	41.0
21	38.34503189234763	40.0	38.0	41.0	34.0	41.0
22	38.30422096310021	40.0	38.0	41.0	34.0	41.0
23	38.25094472591032	40.0	38.0	41.0	34.0	41.0
24	38.21703351195098	40.0	38.0	41.0	33.0	41.0
25	38.18413044982433	40.0	38.0	41.0	33.0	41.0
26	37.99513657715283	40.0	37.0	41.0	33.0	41.0
27	37.89490670623419	40.0	37.0	41.0	33.0	41.0
28	37.790790299806005	40.0	37.0	41.0	33.0	41.0
29	37.716049705421824	40.0	37.0	41.0	32.0	41.0
30	37.652411594514874	40.0	37.0	41.0	32.0	41.0
31	37.560969096840495	40.0	37.0	41.0	32.0	41.0
32	37.465565233881485	40.0	37.0	41.0	32.0	41.0
33	37.39285170634951	40.0	37.0	41.0	31.0	41.0
34	37.25833493499804	39.0	36.0	41.0	31.0	41.0
35	37.15872625371907	39.0	36.0	41.0	31.0	41.0
36	37.04386101138079	39.0	36.0	41.0	31.0	41.0
37	36.957900050997004	39.0	36.0	41.0	31.0	41.0
38	36.8466240751988	39.0	35.0	41.0	30.0	41.0
39	36.74119148478162	39.0	35.0	41.0	30.0	41.0
40	36.61682747380317	39.0	35.0	40.0	30.0	41.0
41	36.45970493492116	39.0	35.0	40.0	30.0	41.0
42	36.39180306443305	39.0	35.0	40.0	30.0	41.0
43	35.0713988811411	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	0.0
12	1.0
13	2.0
14	4.0
15	3.0
16	18.0
17	40.0
18	95.0
19	169.0
20	395.0
21	748.0
22	1433.0
23	2446.0
24	3853.0
25	5800.0
26	8821.0
27	12519.0
28	17414.0
29	23633.0
30	32092.0
31	41351.0
32	53996.0
33	69987.0
34	94502.0
35	126388.0
36	173278.0
37	261823.0
38	468773.0
39	551504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36534100082262	17.258206289288836	11.42461028294368	27.951842426944868
2	20.141766546477747	18.370607274376695	33.50703066739446	27.980595511751094
3	21.157350103403473	20.523090879736763	28.2021121472814	30.117446869578362
4	15.704258377987745	14.152924383487223	32.35967495175786	37.78314228676717
5	16.293517230068243	35.23406087350949	32.168448999151764	16.30397289727051
6	37.47833908651296	32.62142540470864	14.617381521658352	15.282853987120054
7	32.16040223566766	28.639866331470277	19.04212762576912	20.15760380709294
8	29.467042865672866	32.01540673314216	18.087740473938993	20.42980992724598
9	28.265717456095167	13.074350556994919	17.32586060647995	41.33407138042997
10	19.237812612917363	24.271088798853977	30.03672296838442	26.45437561984424
11	39.368252186592656	19.81953723421976	19.51693792460131	21.29527265458627
12	24.301225721966382	22.536985641396242	26.875267478005938	26.286521158631437
13	32.020019527496096	17.249954512722343	23.376770480166265	27.353255479615292
14	24.01461743277493	18.571058815690677	22.83312703891917	34.581196712615224
15	28.531363157611494	24.500805957680175	19.706318759465837	27.261512125242493
16	27.562830103095955	23.77413708712287	20.872740691765394	27.79029211801578
17	25.995402581627243	24.323367134865293	22.943782850143123	26.73744743336434
18	26.22850245631299	22.313367621771366	24.082886789213237	27.375243132702405
19	27.472265573946935	22.70632644745643	22.894989736532562	26.92641824206407
20	26.937335188701727	23.083858038691094	23.083858038691094	26.894948733916085
21	28.99412893785285	22.009384473846737	22.567173817779246	26.429312770521168
22	28.38411251117962	22.74912292840687	22.35749668775739	26.50926787265612
23	27.07136249131898	22.84081502950907	22.768752931046414	27.31906954812554
24	27.625154080144736	22.87740986471699	22.562150997260513	26.935285057877756
25	27.398307104472103	22.50033955291772	22.986989357258363	27.114363985351815
26	28.018215412371	22.655226936668896	23.324543397425547	26.002014253534554
27	28.628283092314827	22.255656439076517	22.185029432190642	26.931031036418013
28	27.026362119732767	22.164118097786115	23.553645517004554	27.255874265476564
29	26.617771046514903	22.525094882617196	23.51110530240711	27.34602876846079
30	26.417165745389127	23.143670605480512	23.6418011424354	26.79736250669496
31	26.90279048431778	22.84983560513455	23.107434543166786	27.139939367380883
32	26.2281949366894	22.46425725041579	23.37277272505952	27.934775087835295
33	27.18739989595586	22.667015188906742	23.073966157465424	27.071618757671974
34	27.342338532977635	22.92891940166932	23.320545642318802	26.408196423034248
35	27.764204203280723	22.439604427257514	23.716425904428025	26.079765465033738
36	27.151317593453932	23.08262796019671	22.56707131123805	27.19898313511131
37	26.66487280219569	22.726007703366573	23.198819124645393	27.410300369792346
38	27.21189895930234	22.290457409813463	23.98258413865035	26.51505949223385
39	25.995710101250836	22.34960368408509	24.2234745104672	27.431211704196873
40	26.494558183994116	21.879508686148036	25.078174050981627	26.547759078876222
41	25.472875487867068	21.88929806083251	25.43289793679959	27.204928514500832
42	25.88295290593231	22.326949738480188	24.817704929795834	26.972392425791668
43	25.46677634866575	20.99861872435735	25.71243327464834	27.822171652328564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	50.0
2	59.0
3	150.5
4	242.0
5	242.0
6	335.5
7	429.0
8	403.0
9	377.0
10	521.5
11	666.0
12	666.0
13	1242.0
14	1818.0
15	2994.5
16	4171.0
17	3984.5
18	3798.0
19	3798.0
20	4581.0
21	5364.0
22	5068.0
23	4772.0
24	5888.5
25	7005.0
26	7005.0
27	8736.5
28	10468.0
29	13003.5
30	15539.0
31	19291.5
32	23044.0
33	23044.0
34	28417.0
35	33790.0
36	40975.5
37	48161.0
38	57709.5
39	67258.0
40	67258.0
41	74354.5
42	81451.0
43	88035.5
44	94620.0
45	104668.0
46	114716.0
47	114716.0
48	123794.5
49	132873.0
50	145725.0
51	158577.0
52	170644.5
53	182712.0
54	182712.0
55	184963.5
56	187215.0
57	177164.0
58	167113.0
59	153605.5
60	140098.0
61	140098.0
62	131075.5
63	122053.0
64	106823.5
65	91594.0
66	78514.0
67	65434.0
68	65434.0
69	55457.5
70	45481.0
71	42150.0
72	38819.0
73	39351.0
74	39883.0
75	39883.0
76	37209.5
77	34536.0
78	25972.0
79	17408.0
80	11244.0
81	5080.0
82	5080.0
83	3484.5
84	1889.0
85	1495.0
86	1101.0
87	946.5
88	792.0
89	792.0
90	659.0
91	526.0
92	304.5
93	83.0
94	54.5
95	26.0
96	26.0
97	18.5
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1951095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.76951423278583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41831706359396	37.26476821651633
2	10.223395057706782	9.358396512022056
3	3.059128527766394	4.200444800745753
4	1.3932381271169239	2.550713291549517
5	0.8024628274103542	1.8364166900219883
6	0.4954525105726529	1.3605972440594705
7	0.36515770076318654	1.1699163407604412
8	0.2780369219048271	1.0180491883490355
9	0.2085260076978588	0.858972066950984
>10	1.4240976144127697	12.955705748546539
>50	0.1757812927407869	5.623261308446558
>100	0.13518603236853408	12.077270374211126
>500	0.015269645336085148	4.729185273281241
>1k	0.005838393804973733	4.553847548323232
>5k	1.1227680394180257E-4	0.44245539621584173
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	8610	0.44129065986023236	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4404	0.22571940371944985	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3089	0.15832135288133073	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2964	0.15191469405641447	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2930	0.15017208285603725	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2905	0.148890751091054	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2612	0.13387354280545027	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2606	0.1335660231818543	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2557	0.1310546129224871	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2213	0.11342348783631756	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2205	0.11301346167152292	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2174	0.11142461028294368	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2150	0.11019453178855976	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	0.10932322618837115	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2011	0.10307032717525287	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1976	0.10127646270427632	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1967	0.10081518326888234	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1959	0.1004051571040877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	5.125327059933012E-5	0.0	0.0	0.0	0.0
6	1.0250654119866024E-4	0.0	0.0	0.0	0.0
7	1.0250654119866024E-4	0.0	0.0	0.0	0.0
8	1.0250654119866024E-4	0.0	0.0	5.125327059933012E-5	0.0
9	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
10	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
11	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
12	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
13	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
14	1.0250654119866024E-4	0.0	0.0	1.0250654119866024E-4	0.0
15	1.0250654119866024E-4	0.0	0.0	2.562663529966506E-4	5.125327059933012E-5
16	1.0250654119866024E-4	0.0	0.0	5.125327059933012E-4	5.125327059933012E-5
17	1.0250654119866024E-4	0.0	0.0	7.687990589899518E-4	5.125327059933012E-5
18	1.0250654119866024E-4	0.0	0.0	8.200523295892819E-4	5.125327059933012E-5
19	1.0250654119866024E-4	0.0	0.0	8.713056001886121E-4	5.125327059933012E-5
20	1.0250654119866024E-4	0.0	0.0	0.0010250654119866023	1.0250654119866024E-4
21	1.0250654119866024E-4	0.0	0.0	0.0014863448473805734	1.0250654119866024E-4
22	1.5375981179799035E-4	0.0	0.0	0.0020501308239732046	1.0250654119866024E-4
23	1.5375981179799035E-4	0.0	0.0	0.0028189298829631565	1.0250654119866024E-4
24	1.5375981179799035E-4	5.125327059933012E-5	0.0	0.00435652800094306	1.5375981179799035E-4
25	1.5375981179799035E-4	5.125327059933012E-5	0.0	0.004971567248135022	1.5375981179799035E-4
26	2.0501308239732048E-4	1.5375981179799035E-4	0.0	0.006457912095515595	2.0501308239732048E-4
27	2.0501308239732048E-4	1.5375981179799035E-4	0.0	0.011121959720054636	2.0501308239732048E-4
28	2.562663529966506E-4	1.5375981179799035E-4	0.0	0.031213241794992044	2.0501308239732048E-4
29	2.562663529966506E-4	1.5375981179799035E-4	0.0	0.061913950883990786	2.0501308239732048E-4
30	2.562663529966506E-4	1.5375981179799035E-4	0.0	0.10778562807039124	2.0501308239732048E-4
31	2.562663529966506E-4	1.5375981179799035E-4	0.0	0.2638518370453515	2.0501308239732048E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1240	0.0	22.379032	1
CAGGACA	1860	0.0	21.682795	4
GACAGGC	1785	0.0	20.935575	7
CCAGGAC	1940	0.0	20.597939	3
TCCAGGA	2200	0.0	18.5	2
ACAGGCT	2015	0.0	18.454094	8
TGCATCA	1975	0.0	18.265821	14
GTACTAG	165	3.6379788E-12	17.939394	1
GCATCAG	1970	0.0	17.936548	15
TGCGCTA	290	0.0	17.862068	10
GCGCTAT	290	0.0	17.862068	11
TTGTGCG	295	0.0	17.559322	7
GCTGCAT	2050	0.0	17.507318	12
ATCAGAA	2060	0.0	17.42233	17
CTGCATC	2060	0.0	17.42233	13
GGACAGG	2275	0.0	17.076923	6
ATCAAGC	2095	0.0	17.04296	30
GGACCGT	470	0.0	16.925533	6
TATACAG	155	4.0199666E-10	16.709679	5
CCATCAA	2215	0.0	16.286682	28
>>END_MODULE