FastQCFastQC Report
Fri 10 Feb 2017
ERR1630274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169807
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129161.1041137555169356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94420.8071416908943099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80540.6884896397439919No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41350.35347711203642995No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.10369231847646664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA250.005496382729.629
GACCGTG652.6829148E-622.769237
CGCATCG753.740879E-722.213
ATGGTCG603.7263595E-521.58333236
CCGAATT1403.6379788E-1219.8214314
TCGTTTA902.1522828E-618.530
ATTAGAC1103.85171E-818.53
GTCGAAA801.616711E-518.515
CATCGTT1002.87504E-718.528
ACCGTGA1002.87504E-718.58
TATACGG609.23695E-418.52
TATACCG701.2192206E-418.55
CGTTTAT902.1522828E-618.531
TGTCGAA801.616711E-518.514
TGGTCGG801.616711E-518.537
GCATCGT902.1522828E-618.527
CGAATTA1552.0008883E-1117.90322715
TACGGTG1258.578354E-917.7628
TAACGCC953.6064976E-617.5263164
ATCGAGC953.6064976E-617.52631626