FastQCFastQC Report
Fri 10 Feb 2017
ERR1630273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513375
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153461.0140249442471299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110020.7269843891963327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101400.6700256050218882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49770.32886759725778475No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA29740.1965144131494177No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16340.10797059552325101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTA1900.020.4473698
ACGACGG1950.019.9230756
ACGGTAT1950.019.9230759
TACGACG2100.019.3809535
AACGTTA902.1531323E-618.58
TATCGTC902.1531323E-618.513
CGACGGT2150.018.0697697
GACGGAC2400.017.7291667
CGTATTA1156.4088454E-817.69565215
ACGTTAT953.6079127E-617.5263169
CTAGTAC852.7240385E-517.4117643
CGTGTCG852.7240385E-517.4117649
CGAACTA752.0678113E-417.26666629
TAGGTCG650.001580246317.07692321
ACGGACC2500.017.028
CGGACCA2400.016.9583329
TTACTCG1757.2759576E-1216.91428619
CGATGCG2250.016.44444533
CCGTATT1251.6586091E-716.27999914
AATGCGA803.3834507E-416.187520
Produced by FastQC (version 0.11.5)