##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1513375
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860226315354755	33.0	31.0	34.0	30.0	34.0
2	32.02933972082266	33.0	31.0	34.0	30.0	34.0
3	32.162216899314444	34.0	31.0	34.0	30.0	34.0
4	35.824411001899726	37.0	35.0	37.0	35.0	37.0
5	35.75085950276699	37.0	35.0	37.0	35.0	37.0
6	35.826420417940035	37.0	35.0	37.0	35.0	37.0
7	35.7999332617494	37.0	35.0	37.0	35.0	37.0
8	35.79563095729743	37.0	35.0	37.0	35.0	37.0
9	37.488038324935985	39.0	37.0	39.0	35.0	39.0
10	37.40350739241761	39.0	37.0	39.0	34.0	39.0
11	37.489866027917735	39.0	37.0	39.0	35.0	39.0
12	37.38130073511192	39.0	37.0	39.0	34.0	39.0
13	37.428482035186256	39.0	37.0	39.0	34.0	39.0
14	38.612120591393406	40.0	38.0	41.0	34.0	41.0
15	38.59524176096473	40.0	38.0	41.0	34.0	41.0
16	38.57572577847526	40.0	38.0	41.0	34.0	41.0
17	38.583440323779634	40.0	38.0	41.0	34.0	41.0
18	38.58991195176344	40.0	38.0	41.0	34.0	41.0
19	38.618804988849426	40.0	38.0	41.0	34.0	41.0
20	38.58881704798877	40.0	38.0	41.0	34.0	41.0
21	38.56229751383497	40.0	38.0	41.0	34.0	41.0
22	38.531696373998514	40.0	38.0	41.0	34.0	41.0
23	38.47992863632609	40.0	38.0	41.0	34.0	41.0
24	38.45790170975469	40.0	38.0	41.0	34.0	41.0
25	38.428740398116794	40.0	38.0	41.0	34.0	41.0
26	38.27244602296192	40.0	38.0	41.0	34.0	41.0
27	38.1703827537788	40.0	38.0	41.0	34.0	41.0
28	38.07460873874618	40.0	37.0	41.0	33.0	41.0
29	37.98888841166267	40.0	37.0	41.0	33.0	41.0
30	37.89078120095812	40.0	37.0	41.0	33.0	41.0
31	37.746477244569256	40.0	37.0	41.0	33.0	41.0
32	37.61846733294788	40.0	37.0	41.0	33.0	41.0
33	37.5274026596184	40.0	36.0	41.0	33.0	41.0
34	37.433367142975136	40.0	36.0	41.0	32.0	41.0
35	37.32946295531511	39.0	36.0	41.0	32.0	41.0
36	37.21591343850665	39.0	36.0	41.0	32.0	41.0
37	37.13049772858677	39.0	36.0	41.0	31.0	41.0
38	37.00318295201123	39.0	35.0	41.0	31.0	41.0
39	36.869033121334766	39.0	35.0	41.0	31.0	41.0
40	36.74031353762286	39.0	35.0	41.0	31.0	41.0
41	36.56117948294375	39.0	35.0	41.0	30.0	41.0
42	36.45244371025027	39.0	35.0	41.0	30.0	41.0
43	35.339360700421246	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	8.0
12	5.0
13	1.0
14	6.0
15	7.0
16	8.0
17	26.0
18	44.0
19	96.0
20	209.0
21	379.0
22	774.0
23	1316.0
24	2275.0
25	3594.0
26	5626.0
27	8351.0
28	12098.0
29	17011.0
30	23127.0
31	30534.0
32	40079.0
33	53097.0
34	70517.0
35	94559.0
36	134080.0
37	210123.0
38	348570.0
39	456847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69604361113406	19.082910712810772	13.113207235483603	24.10783844057157
2	17.227455191211696	21.95655405963492	35.702387048814735	25.11360370033865
3	19.069563062691007	24.26886924919468	30.970975468737095	25.690592219377216
4	13.533195671925332	15.717056248451309	35.34523829189725	35.404509787726106
5	13.016866275708269	38.969653919220285	34.336235235813994	13.677244569257455
6	32.8845130915999	36.33785413397208	16.222086396299662	14.555546378128357
7	28.41734533740811	32.29503592962749	21.19534153795325	18.09227719501115
8	28.493268357148754	34.387775666969524	19.302221855125133	17.816734120756585
9	26.947517964813745	14.233616915833815	19.98110184190964	38.8377632774428
10	17.316131163789546	28.288494259519286	32.13876269926489	22.256611877426284
11	34.57748410010738	23.404245477822748	20.98118443875444	21.037085983315436
12	20.812290410506318	27.443825885851158	29.369257454365243	22.374626249277277
13	30.068555381184435	19.879408606591227	25.908581812174774	24.143454200049558
14	22.55647146279012	21.291087800446025	26.287932600974646	29.864508135789215
15	24.661831998017675	28.446221194350375	23.213413727595604	23.678533080036342
16	23.381713058561164	27.460014867432065	25.322606756421905	23.835665317584866
17	22.223308829602708	28.112001321549517	26.676798546295533	22.987891302552242
18	21.89226067564219	25.943140332039317	28.508466176592055	23.656132815726437
19	22.644684893037088	26.421409102172298	29.269943008177083	21.663962996613527
20	23.065201949285537	25.81805567027339	28.89092260675642	22.225819773684645
21	23.325613281572643	26.18888246468985	28.06535062360618	22.420153630131328
22	23.53421987280086	26.015032625753697	27.950904435450564	22.499843065994877
23	22.65314280994466	26.397621210869744	28.308383579747254	22.64085239943834
24	22.16727513009003	26.907012472123565	28.248913851490876	22.676798546295533
25	22.79025357231354	26.113818452135128	28.42983398034195	22.666093995209383
26	22.306632526637483	27.125002064921123	28.36283141983976	22.205533988601637
27	22.722920624432145	26.712414305773517	27.813132898323286	22.75153217147105
28	22.110250268439746	26.939588667712894	28.931692409349964	22.018468654497397
29	22.090493103163457	27.125662839679528	28.777797968117618	22.0060460890394
30	21.824068720574875	27.933922524159577	28.391244734451142	21.850764020814406
31	22.325993227058728	27.299710911043196	28.258296853060212	22.11599900883786
32	21.860477409762947	27.27202444866606	28.254001817130586	22.61349632444041
33	21.59841414057983	27.25167258610721	28.615445609977698	22.53446766333526
34	22.58230775584373	26.7939539109606	28.263120508796565	22.360617824399107
35	22.30445196993475	27.04848434789791	28.803303873792018	21.84375980837532
36	21.74391674238044	27.334335508383578	28.169092260675644	22.752655488560336
37	22.34872387874783	26.48319154208309	28.43300569918229	22.735078879986784
38	21.616255059056744	26.917915255637237	28.97794664243826	22.48788304286776
39	21.886181547864872	26.847410588915505	28.894358635500122	22.3720492277195
40	21.68603287354423	26.380639299578757	29.56504501528042	22.368282811596597
41	20.94682415131742	26.747501445444783	29.72594366895185	22.57973073428595
42	21.292937969769554	26.55164780705377	29.430246964565953	22.72516725861072
43	20.99988436441728	26.07754191789874	29.721516478070537	23.201057239613444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	570.0
1	500.5
2	431.0
3	1086.0
4	1741.0
5	1741.0
6	2392.0
7	3043.0
8	2871.0
9	2699.0
10	3674.5
11	4650.0
12	4650.0
13	8829.0
14	13008.0
15	21886.0
16	30764.0
17	27114.0
18	23464.0
19	23464.0
20	26217.0
21	28970.0
22	24543.0
23	20116.0
24	22889.5
25	25663.0
26	25663.0
27	28637.0
28	31611.0
29	37403.5
30	43196.0
31	46732.0
32	50268.0
33	50268.0
34	54356.5
35	58445.0
36	63967.0
37	69489.0
38	73771.5
39	78054.0
40	78054.0
41	84415.5
42	90777.0
43	90895.0
44	91013.0
45	95193.0
46	99373.0
47	99373.0
48	102546.5
49	105720.0
50	104267.5
51	102815.0
52	103151.0
53	103487.0
54	103487.0
55	97587.0
56	91687.0
57	86195.0
58	80703.0
59	74582.0
60	68461.0
61	68461.0
62	64152.5
63	59844.0
64	52835.5
65	45827.0
66	39240.5
67	32654.0
68	32654.0
69	27246.5
70	21839.0
71	18417.5
72	14996.0
73	11737.5
74	8479.0
75	8479.0
76	6466.0
77	4453.0
78	3521.5
79	2590.0
80	1902.0
81	1214.0
82	1214.0
83	877.0
84	540.0
85	439.5
86	339.0
87	271.5
88	204.0
89	204.0
90	175.5
91	147.0
92	85.5
93	24.0
94	13.5
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1513375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.37281074375723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02787717678754	34.79253548825031
2	13.682323977350913	12.68975640672931
3	4.5370297964957915	6.311844722750602
4	2.109337752275198	3.9126368152368007
5	1.1283154621590463	2.6161579692978214
6	0.667784924367507	1.858023834913718
7	0.4640554660361379	1.5063689410769918
8	0.31902199117774366	1.1835157135985677
9	0.24636983263949605	1.0282375459766242
>10	1.512928731351124	13.624590689171043
>50	0.1835687697287446	5.957683453838604
>100	0.11540233197652329	9.677855611799306
>500	0.0041316629041250165	1.2352048904928297
>1k	0.0014247113462500056	1.1949034900221072
>5k	0.0	0.0
>10k+	4.2741340387500166E-4	2.410684426845441
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15346	1.0140249442471299	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11002	0.7269843891963327	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10140	0.6700256050218882	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4977	0.32886759725778475	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2974	0.1965144131494177	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1634	0.10797059552325101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.60774758404229E-5	0.0
13	0.0	0.0	0.0	1.321549516808458E-4	0.0
14	0.0	0.0	0.0	1.321549516808458E-4	0.0
15	0.0	0.0	0.0	5.946972825638061E-4	0.0
16	0.0	0.0	0.0	0.001255472040968035	0.0
17	0.0	0.0	0.0	0.0021805567027339554	0.0
18	0.0	0.0	0.0	0.0023127116544148013	0.0
19	0.0	0.0	0.0	0.0027752539852977614	0.0
20	0.0	0.0	0.0	0.0029734864128190303	0.0
21	0.0	0.0	0.0	0.004162880977946643	0.0
22	0.0	0.0	0.0	0.005814817873957215	0.0
23	0.0	0.0	0.0	0.0086561493350954	0.0
24	0.0	0.0	0.0	0.012819030313042042	0.0
25	0.0	0.0	0.0	0.014008424878169654	0.0
26	0.0	0.0	0.0	0.01665152391178657	0.0
27	0.0	0.0	0.0	0.026827455191211696	0.0
28	0.0	0.0	0.0	0.0571570166019658	0.0
29	0.0	0.0	0.0	0.10321301726274057	0.0
30	0.0	0.0	0.0	0.18547947468406706	0.0
31	0.0	0.0	0.0	0.4157594779879409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGTA	190	0.0	20.447369	8
ACGACGG	195	0.0	19.923075	6
ACGGTAT	195	0.0	19.923075	9
TACGACG	210	0.0	19.380953	5
AACGTTA	90	2.1531323E-6	18.5	8
TATCGTC	90	2.1531323E-6	18.5	13
CGACGGT	215	0.0	18.069769	7
GACGGAC	240	0.0	17.729166	7
CGTATTA	115	6.4088454E-8	17.695652	15
ACGTTAT	95	3.6079127E-6	17.526316	9
CTAGTAC	85	2.7240385E-5	17.411764	3
CGTGTCG	85	2.7240385E-5	17.411764	9
CGAACTA	75	2.0678113E-4	17.266666	29
TAGGTCG	65	0.0015802463	17.076923	21
ACGGACC	250	0.0	17.02	8
CGGACCA	240	0.0	16.958332	9
TTACTCG	175	7.2759576E-12	16.914286	19
CGATGCG	225	0.0	16.444445	33
CCGTATT	125	1.6586091E-7	16.279999	14
AATGCGA	80	3.3834507E-4	16.1875	20
>>END_MODULE