FastQCFastQC Report
Fri 10 Feb 2017
ERR1630272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105754
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22432.1209599636893164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19651.8580857461656295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13741.2992416362501655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.7612005219660721No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2420.2288329519450801No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.22694177052404638No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2390.22599617981352954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.22505058910301268No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2320.2193770448399115No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2290.21654027270836093No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2160.20424759347164173No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2140.20235641205060803No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2090.1976284584980237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1960.18533577926130454No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1920.1815534164192371No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1900.17966223499820336No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.17020632789303478No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.16358719291941676No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1720.1626416022088999No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1650.15602246723528188No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1630.15413128581424815No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1620.1531856951037313No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1590.15034892297218072No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1560.14751215084063016No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1480.13994742515649528No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.13616506231442782No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1360.12860033663029294No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1330.12576356449874238No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1310.12387238307770863No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1250.1181988388146075No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.11725324810409063No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1230.11630765739357375No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1220.1153620666830569No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1200.11347088526202319No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1190.11252529455150634No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1160.10968852241995575No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1140.10779734099892202No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1140.10779734099892202No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1140.10779734099892202No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1110.10496056886737146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.10496056886737146No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1100.10401497815685459No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1100.10401497815685459No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1100.10401497815685459No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1100.10401497815685459No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1080.10212379673582087No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1070.10117820602530402No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1070.10117820602530402No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1060.10023261531478715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC250.00547408429.59999817
TACCCTA250.00547408429.59999835
ACGTTCG250.00547408429.59999822
TTCGTTA250.00547408429.59999830
CCGCTTT250.00547408429.59999814
CGGGGTC250.00547408429.59999825
CAGTGGC250.00547408429.59999826
AGTTCGC250.00547408429.59999814
ATGCCCC405.89005E-527.7537
TTAGATG751.3438694E-824.6666663
CTTAGAT751.3438694E-824.6666662
TCTTATA2650.023.7358537
GTGTTTC400.001919677523.12537
TGTTTCA802.6670023E-823.1258
GATGTTT802.6670023E-823.1256
ACTTAGA802.6670023E-823.1251
ATGTTTC855.0695235E-821.7647067
GTTTCAG909.2757546E-820.5555559
TTTCAGT909.2757546E-820.55555510
TAGATGT909.2757546E-820.5555554
Produced by FastQC (version 0.11.5)