FastQCFastQC Report
Fri 10 Feb 2017
ERR1630271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144815
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG49230.2295302858288477No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG35960.16766014784491903No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT27080.12625797562959976No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24460.11404246986336816No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA23480.10947331121798384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG450.003826872420.5555555
GGTATCA13550.019.6605171
ACCGGAC701.2198227E-418.53
ACGGACC2800.018.58
GACGGAC3050.016.9836067
CGAATTA1107.815361E-716.81818215
CGCAAGA3300.016.8181822
TCTAGCG3250.015.93846128
AAGACGG3400.015.7794125
TCGCCAT7000.015.59285713
CGGACCA3450.015.5507249
TATACTG2055.456968E-1215.3414635
CGAACGA1701.4879333E-915.23529416
CTAGCGG3400.015.23529429
GCTTAGG12200.015.1639341
CGCAATA3650.014.69863136
AGGTCGC7600.014.60526410
TTGACGG3050.014.55737633
CGTTTAG1152.2114984E-514.47826126
GTATAGA1803.3342076E-914.3888881
Produced by FastQC (version 0.11.5)