##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144815
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845843580914906	31.0	31.0	34.0	30.0	34.0
2	32.038870485333234	33.0	31.0	34.0	30.0	34.0
3	32.18108787937421	34.0	31.0	34.0	30.0	34.0
4	35.83186801658884	37.0	35.0	37.0	35.0	37.0
5	35.74814004937489	37.0	35.0	37.0	35.0	37.0
6	35.811517077230434	37.0	35.0	37.0	35.0	37.0
7	35.78893051382054	37.0	35.0	37.0	35.0	37.0
8	35.7658040437054	37.0	35.0	37.0	35.0	37.0
9	37.48304119469511	39.0	37.0	39.0	35.0	39.0
10	37.398175600226594	39.0	37.0	39.0	34.0	39.0
11	37.476497040537296	39.0	37.0	39.0	35.0	39.0
12	37.35672773642482	39.0	37.0	39.0	34.0	39.0
13	37.385805302555234	39.0	37.0	39.0	34.0	39.0
14	38.613458503414044	40.0	38.0	41.0	34.0	41.0
15	38.6214815730028	40.0	38.0	41.0	34.0	41.0
16	38.590246245014136	40.0	38.0	41.0	34.0	41.0
17	38.55803041287943	40.0	38.0	41.0	34.0	41.0
18	38.5188787844173	40.0	38.0	41.0	34.0	41.0
19	38.53946051291137	40.0	38.0	41.0	34.0	41.0
20	38.49067588579901	40.0	38.0	41.0	34.0	41.0
21	38.437714208451546	40.0	38.0	41.0	34.0	41.0
22	38.417523655886406	40.0	38.0	41.0	34.0	41.0
23	38.365142914423856	40.0	38.0	41.0	34.0	41.0
24	38.351127719640154	40.0	38.0	41.0	34.0	41.0
25	38.314939516928035	40.0	38.0	41.0	34.0	41.0
26	38.16496434424414	40.0	38.0	41.0	33.0	41.0
27	38.06060009837678	40.0	38.0	41.0	33.0	41.0
28	37.982868452523874	40.0	37.0	41.0	33.0	41.0
29	37.92849686336584	40.0	37.0	41.0	33.0	41.0
30	37.879850709734875	40.0	37.0	41.0	33.0	41.0
31	37.78589668572814	40.0	37.0	41.0	33.0	41.0
32	37.68314563260701	40.0	37.0	41.0	32.0	41.0
33	37.63237062403984	40.0	37.0	41.0	32.0	41.0
34	37.55644519457389	40.0	37.0	41.0	32.0	41.0
35	37.46796017372127	40.0	37.0	41.0	32.0	41.0
36	37.37557691455906	40.0	37.0	41.0	31.0	41.0
37	37.32514552537165	40.0	36.0	41.0	31.0	41.0
38	37.21381191384805	39.0	36.0	41.0	31.0	41.0
39	37.12795835538263	39.0	36.0	41.0	31.0	41.0
40	37.040267342404825	39.0	36.0	41.0	31.0	41.0
41	36.917930450878046	39.0	36.0	41.0	30.0	41.0
42	36.87018833792192	39.0	36.0	41.0	30.0	41.0
43	35.65899576420344	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	5.0
11	0.0
12	0.0
13	1.0
14	2.0
15	3.0
16	16.0
17	48.0
18	91.0
19	202.0
20	382.0
21	719.0
22	1255.0
23	2109.0
24	3429.0
25	5337.0
26	8146.0
27	11893.0
28	16872.0
29	24103.0
30	32135.0
31	43207.0
32	56118.0
33	72992.0
34	97446.0
35	132133.0
36	183555.0
37	276228.0
38	509239.0
39	667145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62824532652	18.500057114483067	12.1338670234962	26.737830535500727
2	19.16831987840443	20.86296487109611	33.74435557379075	26.224359676708715
3	20.17796406683094	22.34001533931831	29.673981205838267	27.808039388012485
4	15.219634327436166	15.283369428132495	33.716521005308145	35.78047523912319
5	14.95909903651364	36.20335553416029	33.89028890603619	14.947256523289887
6	35.767793492678855	34.06997806337609	15.366033900359705	14.796194543585345
7	29.850220182160232	30.084692619176945	20.633014968656973	19.43207223000585
8	27.103223354928048	33.48461289202099	19.871597317251137	19.540566435799825
9	26.869963143674397	14.091471758636526	18.790012192193732	40.248552905495345
10	17.126372204595736	26.61539573343156	32.72720491044682	23.531027151525887
11	36.47699218813744	21.29549634816989	20.70686749206808	21.520643971624594
12	21.73012590829512	25.28148115338619	28.251900513564106	24.736492424754584
13	30.450738175553603	19.130554383478295	24.815613467828225	25.603093973139874
14	23.317861913498366	20.583919825252995	23.685352815977133	32.41286544527151
15	26.09068847429732	27.435466462142422	21.376762098362796	25.097082965197465
16	26.090781722432936	25.980842170536857	22.68195625263717	25.246419854393036
17	24.642451679981722	26.0307299230936	24.125017775425853	25.201800621498826
18	24.053356583201815	24.6989600501675	25.81453412065843	25.433149245972263
19	25.307870375766672	25.054515191286896	25.196858470310957	24.44075596263547
20	25.569757764655694	24.223627678844096	25.46793080055856	24.738683755941654
21	25.549476295158325	25.16711231504815	24.25729025580295	25.026121133990575
22	25.496138361583633	24.53293174469593	24.609814832514694	25.361115061205748
23	24.57890307555663	25.068222667223043	24.917533680060984	25.435340577159337
24	25.421446604951942	25.379624816126334	24.725022904073313	24.473905674848414
25	25.70370871147395	24.73439434170313	24.663246014225003	24.898650932597917
26	25.568592162960442	25.392493058841904	24.317062310735423	24.72185246746223
27	25.770474376577933	24.32261057480482	24.414506612458418	25.492408436158833
28	24.712993894578318	24.727493979667244	25.231546776761633	25.327965348992805
29	24.381543396516715	25.257423134396205	25.106454402827282	25.25457906625979
30	24.245913983257296	26.213263148569922	24.941405202779727	24.59941766539305
31	25.519077402946177	25.191962943190905	24.497264332821246	24.791695321041676
32	24.351284376507998	25.09256043061989	24.804796684096296	25.75135850877582
33	24.385226697873712	24.7008250128799	25.24054522184897	25.67340306739742
34	25.129533316393253	24.52612463079566	25.266468203551355	25.077873849259724
35	25.44513163139944	24.661660795919463	25.33430622221497	24.558901350466126
36	24.477542352137597	25.65172287586575	25.027286735685827	24.843448036310825
37	25.96368451358276	24.19663234358208	24.803677706468857	25.036005436366302
38	24.837806524105808	24.718448910512095	25.76963514335735	24.674109422024742
39	25.10370358282649	24.427747847716468	25.46895653005038	24.99959203940666
40	24.46346188365896	24.042586423537696	26.458365873047324	25.03558581975602
41	23.97190433673767	24.436746292803807	26.7348932192287	24.85645615122983
42	24.9770259905866	23.80559628685924	26.362227045223012	24.855150677331146
43	24.054475560829257	23.630149919690044	26.615069364956884	25.700305154523818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	49.5
2	68.0
3	170.0
4	272.0
5	272.0
6	386.0
7	500.0
8	487.0
9	474.0
10	728.5
11	983.0
12	983.0
13	1673.0
14	2363.0
15	3865.0
16	5367.0
17	5635.5
18	5904.0
19	5904.0
20	7680.0
21	9456.0
22	11033.5
23	12611.0
24	15217.0
25	17823.0
26	17823.0
27	21313.5
28	24804.0
29	33893.0
30	42982.0
31	47940.0
32	52898.0
33	52898.0
34	60157.0
35	67416.0
36	74983.5
37	82551.0
38	97109.0
39	111667.0
40	111667.0
41	119545.0
42	127423.0
43	124196.5
44	120970.0
45	132332.5
46	143695.0
47	143695.0
48	153507.0
49	163319.0
50	169257.5
51	175196.0
52	186628.0
53	198060.0
54	198060.0
55	179093.0
56	160126.0
57	152470.0
58	144814.0
59	132084.0
60	119354.0
61	119354.0
62	110845.5
63	102337.0
64	90883.0
65	79429.0
66	69203.0
67	58977.0
68	58977.0
69	49723.0
70	40469.0
71	35527.0
72	30585.0
73	24316.5
74	18048.0
75	18048.0
76	13814.0
77	9580.0
78	7974.5
79	6369.0
80	4908.0
81	3447.0
82	3447.0
83	2579.5
84	1712.0
85	1447.5
86	1183.0
87	1001.5
88	820.0
89	820.0
90	714.5
91	609.0
92	344.0
93	79.0
94	55.0
95	31.0
96	31.0
97	21.0
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2144815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.917583367013336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0653389895204	41.46420983918608
2	10.171258555376745	10.154492937709326
3	2.7707489714345517	4.149272783119521
4	1.1481421569589318	2.2924992734872
5	0.6358283210082394	1.5869506610518453
6	0.39478019775110007	1.1823884059731928
7	0.2667443642536996	0.9320663828220515
8	0.19291959417767857	0.770406394039572
9	0.14771821051919187	0.6636362479576009
>10	0.9397205089834955	9.04176606263474
>50	0.11213965941697457	3.969705448768169
>100	0.1331842276459879	14.335321255064487
>500	0.016036845174469974	5.4334279417939815
>1k	0.005439397778475196	4.023856366392335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4923	0.2295302858288477	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3596	0.16766014784491903	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2708	0.12625797562959976	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2446	0.11404246986336816	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2348	0.10947331121798384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	9.324813562008844E-5	0.0
16	0.0	0.0	0.0	4.662406781004422E-4	0.0
17	0.0	0.0	0.0	6.993610171506634E-4	0.0
18	0.0	0.0	0.0	7.459850849607075E-4	0.0
19	0.0	0.0	0.0	8.39233220580796E-4	0.0
20	0.0	0.0	0.0	9.791054240109287E-4	0.0
21	0.0	0.0	0.0	0.0011189776274410613	0.0
22	0.0	0.0	0.0	0.0013054738986812383	0.0
23	0.0	0.0	0.0	0.0021447071192620343	0.0
24	0.0	0.0	0.0	0.0036833013569934937	0.0
25	0.0	0.0	0.0	0.004242790170714024	0.0
26	0.0	0.0	0.0	0.005315143730345041	0.0
27	0.0	0.0	0.0	0.009884302375729376	0.0
28	0.0	0.0	0.0	0.02275254509130158	0.0
29	0.0	0.0	0.0	0.04284751831743064	0.0
30	0.0	0.0	0.0	0.07082195900345717	0.0
31	0.0	0.0	0.0	0.1574961010623294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTG	45	0.0038268724	20.555555	5
GGTATCA	1355	0.0	19.660517	1
ACCGGAC	70	1.2198227E-4	18.5	3
ACGGACC	280	0.0	18.5	8
GACGGAC	305	0.0	16.983606	7
CGAATTA	110	7.815361E-7	16.818182	15
CGCAAGA	330	0.0	16.818182	2
TCTAGCG	325	0.0	15.938461	28
AAGACGG	340	0.0	15.779412	5
TCGCCAT	700	0.0	15.592857	13
CGGACCA	345	0.0	15.550724	9
TATACTG	205	5.456968E-12	15.341463	5
CGAACGA	170	1.4879333E-9	15.235294	16
CTAGCGG	340	0.0	15.235294	29
GCTTAGG	1220	0.0	15.163934	1
CGCAATA	365	0.0	14.698631	36
AGGTCGC	760	0.0	14.605264	10
TTGACGG	305	0.0	14.557376	33
CGTTTAG	115	2.2114984E-5	14.478261	26
GTATAGA	180	3.3342076E-9	14.388888	1
>>END_MODULE