FastQCFastQC Report
Fri 10 Feb 2017
ERR1630270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523612
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.18996581090912548No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG47920.1898865594235564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42030.16654699692345734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35360.1401166264861635No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA33780.13385575912620482No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC32810.1300120620761036No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT30630.12137365014907205No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30590.12121514717793386No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA29450.11669781250049531No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC26150.10362131738159432No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA25900.1026306738119806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.021.8433741
TCGTTTA3700.019.530
CTAGCGG4750.018.69473629
CGCGAAC500.007037156718.528
TCTAGCG4850.018.30927828
CGCAATA5150.017.60194236
AAGACGG5050.017.2178235
TTACTCG1401.87174E-917.17857219
ATAACGC5050.016.8514863
GACGGAC5200.016.7211537
CGCATCG4600.016.4891313
CGTTTAT4400.016.39772831
TAACGCC4750.016.3578954
GCATCGC4750.016.35789514
CCGGTCG4650.016.31182720
GTCTTAT1251.6599006E-716.2799991
GCTTAGG12850.016.2684821
ATCGTTT4700.016.13829829
AACGCCG5000.015.915
GCAATAC5950.015.85714337
Produced by FastQC (version 0.11.5)