##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630270.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2523612
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89622533099383	33.0	31.0	34.0	30.0	34.0
2	32.09353696210035	33.0	31.0	34.0	30.0	34.0
3	32.24236372310799	34.0	31.0	34.0	30.0	34.0
4	35.88103876507165	37.0	35.0	37.0	35.0	37.0
5	35.79646197592974	37.0	35.0	37.0	35.0	37.0
6	35.85668676484341	37.0	35.0	37.0	35.0	37.0
7	35.8296727072149	37.0	35.0	37.0	35.0	37.0
8	35.80861598375662	37.0	35.0	37.0	35.0	37.0
9	37.5272359617881	39.0	37.0	39.0	35.0	39.0
10	37.44367478043376	39.0	37.0	39.0	34.0	39.0
11	37.514301326828374	39.0	37.0	39.0	35.0	39.0
12	37.401411548209474	39.0	37.0	39.0	34.0	39.0
13	37.432999209070175	39.0	37.0	39.0	34.0	39.0
14	38.6685797975283	40.0	38.0	41.0	35.0	41.0
15	38.67389796846742	40.0	38.0	41.0	35.0	41.0
16	38.65342968728949	40.0	38.0	41.0	34.0	41.0
17	38.62429327487744	40.0	38.0	41.0	34.0	41.0
18	38.58865348555959	40.0	38.0	41.0	34.0	41.0
19	38.611638397661764	40.0	38.0	41.0	34.0	41.0
20	38.56766095580462	40.0	38.0	41.0	34.0	41.0
21	38.52196137916605	40.0	38.0	41.0	34.0	41.0
22	38.50306346617467	40.0	38.0	41.0	34.0	41.0
23	38.45022491571604	40.0	38.0	41.0	34.0	41.0
24	38.43400926925375	40.0	38.0	41.0	34.0	41.0
25	38.404993319099766	40.0	38.0	41.0	34.0	41.0
26	38.25876679933366	40.0	38.0	41.0	34.0	41.0
27	38.15991562886846	40.0	38.0	41.0	33.0	41.0
28	38.08069624015102	40.0	38.0	41.0	33.0	41.0
29	38.02866367730063	40.0	38.0	41.0	33.0	41.0
30	37.97056045065565	40.0	37.0	41.0	33.0	41.0
31	37.86965270413994	40.0	37.0	41.0	33.0	41.0
32	37.77350916067922	40.0	37.0	41.0	33.0	41.0
33	37.71013610650132	40.0	37.0	41.0	33.0	41.0
34	37.639526202918674	40.0	37.0	41.0	32.0	41.0
35	37.54909352150806	40.0	37.0	41.0	32.0	41.0
36	37.45492294378058	40.0	37.0	41.0	32.0	41.0
37	37.41031783015772	40.0	37.0	41.0	32.0	41.0
38	37.294028955322766	40.0	36.0	41.0	31.0	41.0
39	37.209438297170884	39.0	36.0	41.0	31.0	41.0
40	37.120385780381454	39.0	36.0	41.0	31.0	41.0
41	36.9925773058616	39.0	36.0	41.0	31.0	41.0
42	36.94067154538812	39.0	36.0	41.0	31.0	41.0
43	35.76617086937295	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	3.0
11	5.0
12	2.0
13	2.0
14	3.0
15	8.0
16	21.0
17	55.0
18	119.0
19	231.0
20	464.0
21	787.0
22	1402.0
23	2492.0
24	3798.0
25	5994.0
26	8945.0
27	13186.0
28	19283.0
29	26994.0
30	36578.0
31	48589.0
32	63859.0
33	83142.0
34	111427.0
35	150024.0
36	211247.0
37	319907.0
38	591367.0
39	823673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38127731204322	19.002881584015295	12.486864066266923	26.128977037674574
2	17.6852067592007	21.180593530225725	35.426642447412675	25.707557263160897
3	19.194828682063644	22.919886258267912	30.867740365793157	27.01754469387529
4	14.220767693290412	15.467235058321169	35.24646419497133	35.065533053417084
5	13.890447501438413	37.34096208133422	34.680014201866214	14.088576215361156
6	34.25661314021332	35.726014934149944	15.963547486697639	14.053824438939108
7	29.13264796648613	31.326487590009876	21.08969207627797	18.451172367226025
8	26.90484908139603	34.61316557378868	20.08058291052666	18.40140243428863
9	26.412459601555234	14.667983826356826	19.368349809717184	39.55120676237076
10	16.49385880238325	27.196811554232585	33.025956446553586	23.283373196830574
11	35.81984869306375	21.904991734070055	21.590640716560234	20.68451885630596
12	21.260637530650513	25.80769151517745	29.249583533443335	23.6820874207287
13	29.58778924810946	19.28703778552329	25.836459804439034	25.28871316192822
14	22.585246860452397	20.86465748300452	24.80924167423518	31.740853982307897
15	24.96366319386657	28.06136601030586	22.28432896974654	24.69064182608103
16	25.163376937500693	26.860626752448475	23.469614187917955	24.50638212213288
17	23.78523322919688	26.952518849965845	25.07342650137977	24.188821419457508
18	23.279925757208318	25.439013604310013	26.7897759243497	24.491284714131968
19	24.41841297315118	25.691746591789865	26.524640079378287	23.365200355680667
20	24.67705019630593	24.842606549659774	26.44388281558338	24.03646043845092
21	24.62696325742626	25.84775314113263	25.227451763583307	24.2978318378578
22	24.635522417867723	25.258359842955258	25.487634390706653	24.618483348470367
23	23.6010527767343	25.636429054862635	26.055392033323667	24.707126135079402
24	24.631956101017114	25.759902869379285	25.806978251807326	23.80116277779627
25	24.71540791532137	25.157314198854657	25.858135085742184	24.269142800081788
26	24.404147705748745	26.05527315609531	25.588204525893836	23.952374612262105
27	24.806428246497482	25.050166190365236	25.37680118813827	24.76660437499901
28	23.69147872176864	25.575722416916708	26.43369899968775	24.299099861626907
29	23.680779771216812	26.018024957877834	26.04560447485588	24.25559079604947
30	23.286939513681183	26.984496824392973	25.974476266557616	23.754087395368227
31	24.51482240534599	25.881712402698987	25.64962442720989	23.953840764745134
32	23.483998332548744	25.80725563200682	25.638648096458567	25.070097938985864
33	23.569669188448938	25.40402407343126	26.308798658430852	24.717508079688955
34	24.300724517081072	25.173402250425188	26.079841116621733	24.44603211587201
35	24.659575243737944	25.407233758596803	26.228715032263278	23.704475965401972
36	23.591265218266518	26.26643873939417	26.01315099151534	24.12914505082398
37	25.039704994270117	24.92122402334432	25.709023415643927	24.330047566741637
38	24.001986042228364	25.545884232599942	26.685639472311905	23.76649025285979
39	24.352594614386046	25.12787227196574	26.28553834741632	24.23399476623189
40	23.83104058785582	24.938342344227244	27.127109872674566	24.103507195242376
41	23.013482262725017	25.643759817277772	27.460837878406032	23.88192004159118
42	24.295692047747437	24.791528967210493	27.031770335534937	23.881008649507134
43	23.439419371916127	24.54133202726885	27.125207837020902	24.894040763794116
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	136.5
2	136.0
3	384.0
4	632.0
5	632.0
6	886.5
7	1141.0
8	1073.5
9	1006.0
10	1507.0
11	2008.0
12	2008.0
13	3377.0
14	4746.0
15	8577.5
16	12409.0
17	12745.0
18	13081.0
19	13081.0
20	15324.0
21	17567.0
22	20120.5
23	22674.0
24	26723.5
25	30773.0
26	30773.0
27	36131.5
28	41490.0
29	55056.5
30	68623.0
31	72931.0
32	77239.0
33	77239.0
34	85787.5
35	94336.0
36	101909.0
37	109482.0
38	125108.0
39	140734.0
40	140734.0
41	152153.0
42	163572.0
43	154537.5
44	145503.0
45	157476.0
46	169449.0
47	169449.0
48	176896.5
49	184344.0
50	188280.0
51	192216.0
52	204291.5
53	216367.0
54	216367.0
55	195180.5
56	173994.0
57	164036.0
58	154078.0
59	140013.0
60	125948.0
61	125948.0
62	115879.5
63	105811.0
64	92992.5
65	80174.0
66	69640.5
67	59107.0
68	59107.0
69	49963.5
70	40820.0
71	36024.0
72	31228.0
73	24719.0
74	18210.0
75	18210.0
76	13889.0
77	9568.0
78	7883.0
79	6198.0
80	4762.5
81	3327.0
82	3327.0
83	2518.5
84	1710.0
85	1581.0
86	1452.0
87	1294.5
88	1137.0
89	1137.0
90	1051.0
91	965.0
92	551.0
93	137.0
94	99.0
95	61.0
96	61.0
97	36.5
98	12.0
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2523612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.3048689613609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.44091324732085	36.44365033802072
2	11.597847191765736	10.508778945136683
3	3.3729097032827187	4.584276963771788
4	1.4020110664067067	2.540717105837348
5	0.7043941803127033	1.5956243018106127
6	0.43905129712089447	1.1934696890026595
7	0.2998212962082074	0.9508355175576747
8	0.22159910190753113	0.8031614619100766
9	0.1697095997439574	0.6919804060096499
>10	1.0517736272756495	9.222704489885926
>50	0.12727054865241555	4.066409431617072
>100	0.14164034485694652	14.006475840964775
>500	0.022021914072334725	6.708677434636981
>1k	0.009036881073507865	6.683238073838238
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4794	0.18996581090912548	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4792	0.1898865594235564	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4203	0.16654699692345734	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3536	0.1401166264861635	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3378	0.13385575912620482	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3281	0.1300120620761036	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3063	0.12137365014907205	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3059	0.12121514717793386	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2945	0.11669781250049531	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2615	0.10362131738159432	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2590	0.1026306738119806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.9625742784548496E-5	0.0
6	0.0	0.0	0.0	3.9625742784548496E-5	0.0
7	0.0	0.0	0.0	3.9625742784548496E-5	0.0
8	0.0	0.0	0.0	3.9625742784548496E-5	0.0
9	0.0	0.0	0.0	3.9625742784548496E-5	0.0
10	0.0	0.0	0.0	3.9625742784548496E-5	0.0
11	0.0	0.0	0.0	7.925148556909699E-5	0.0
12	0.0	0.0	0.0	7.925148556909699E-5	0.0
13	0.0	0.0	0.0	7.925148556909699E-5	0.0
14	0.0	0.0	0.0	1.1887722835364549E-4	0.0
15	0.0	0.0	0.0	1.5850297113819399E-4	3.9625742784548496E-5
16	0.0	0.0	0.0	3.5663168506093647E-4	7.925148556909699E-5
17	0.0	0.0	0.0	4.3588317063003346E-4	7.925148556909699E-5
18	0.0	0.0	0.0	4.3588317063003346E-4	7.925148556909699E-5
19	0.0	0.0	0.0	5.151346561991305E-4	7.925148556909699E-5
20	0.0	0.0	0.0	6.340118845527759E-4	7.925148556909699E-5
21	0.0	0.0	0.0	9.510178268291639E-4	1.1887722835364549E-4
22	0.0	0.0	0.0	0.0012283980263210034	1.1887722835364549E-4
23	0.0	0.0	0.0	0.0017831584253046823	1.1887722835364549E-4
24	0.0	0.0	0.0	0.0029323049660565886	1.1887722835364549E-4
25	0.0	0.0	0.0	0.003328562393902074	1.1887722835364549E-4
26	0.0	0.0	0.0	0.003922948535670301	1.1887722835364549E-4
27	0.0	0.0	0.0	0.007370388157926021	1.5850297113819399E-4
28	0.0	0.0	0.0	0.018544847623168697	1.5850297113819399E-4
29	3.9625742784548496E-5	0.0	0.0	0.03701044376076829	1.5850297113819399E-4
30	3.9625742784548496E-5	0.0	0.0	0.06300493102743211	1.5850297113819399E-4
31	3.9625742784548496E-5	0.0	0.0	0.16563560483941273	1.5850297113819399E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1660	0.0	21.843374	1
TCGTTTA	370	0.0	19.5	30
CTAGCGG	475	0.0	18.694736	29
CGCGAAC	50	0.0070371567	18.5	28
TCTAGCG	485	0.0	18.309278	28
CGCAATA	515	0.0	17.601942	36
AAGACGG	505	0.0	17.217823	5
TTACTCG	140	1.87174E-9	17.178572	19
ATAACGC	505	0.0	16.851486	3
GACGGAC	520	0.0	16.721153	7
CGCATCG	460	0.0	16.48913	13
CGTTTAT	440	0.0	16.397728	31
TAACGCC	475	0.0	16.357895	4
GCATCGC	475	0.0	16.357895	14
CCGGTCG	465	0.0	16.311827	20
GTCTTAT	125	1.6599006E-7	16.279999	1
GCTTAGG	1285	0.0	16.268482	1
ATCGTTT	470	0.0	16.138298	29
AACGCCG	500	0.0	15.91	5
GCAATAC	595	0.0	15.857143	37
>>END_MODULE