FastQCFastQC Report
Fri 10 Feb 2017
ERR1630269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195741
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC64640.5405852939725241No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT43430.3632057443877897No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT31110.26017339875441253No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG28840.2411893545508601No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG28040.23449894249674472No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC22250.1860770852550845No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG22210.18574256465237873No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT21700.18147742696788016No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG21620.18080838576246863No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG21480.17963756365299843No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG21030.17587420687255853No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT20600.17227811039347152No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC20450.17102365813332487No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT19970.1670094109008556No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG19690.1646677666819152No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT19360.16190797170959262No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG19000.15889728628524072No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG17900.14969796971083205No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT16210.13556447424651327No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT15560.1301285144525445No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA15230.1273687194802219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.11674769034431369No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG13920.11641316974160792No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA13830.11566049838551995No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC13730.11482419687875552No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT13710.11465693657740264No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC13550.11331885416657955No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA13440.11239892250913869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13010.10880282603005166No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT13000.10871919587937523No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA12460.10420316774284732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG400.00193123323.12535
GGTTCGC705.102207E-621.14285723
CCAGGAC14750.020.9457633
CAGGACA14850.020.9292934
TGCATCA13950.020.8207914
CGTAGCG450.003825896720.55555515
GGTATCA7700.020.4220771
GACAGGC14900.020.1140947
ACAGGCT14800.019.8758
GCATCAG14650.019.82593715
ATTAGAC759.266287E-619.7333343
CGGTTCG759.266287E-619.73333422
TTAGACT851.2453929E-619.5882364
TCCAGGA15600.019.4487192
GCTGCAT15300.018.86274512
CTGCATC15500.018.50000213
TTACAAC609.2371134E-418.53
TACCGTC801.6167598E-518.57
GCGACTT500.00703504518.49999813
GATCTAG500.00703504518.4999981
Produced by FastQC (version 0.11.5)