##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630269.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195741
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.906442950438265	31.0	31.0	33.0	28.0	34.0
2	31.139569522162407	31.0	31.0	34.0	28.0	34.0
3	31.233405060125897	31.0	31.0	34.0	28.0	34.0
4	35.08539892836325	37.0	35.0	37.0	32.0	37.0
5	34.734852279883356	35.0	35.0	37.0	32.0	37.0
6	34.803219091759836	35.0	35.0	37.0	32.0	37.0
7	34.80454881115559	35.0	35.0	37.0	32.0	37.0
8	34.76084202180907	35.0	35.0	37.0	32.0	37.0
9	36.3595352170746	38.0	35.0	39.0	32.0	39.0
10	36.11066108797808	37.0	35.0	39.0	32.0	39.0
11	36.25810857033421	37.0	35.0	39.0	32.0	39.0
12	36.08723711907512	37.0	35.0	39.0	32.0	39.0
13	36.15139398916655	37.0	35.0	39.0	32.0	39.0
14	37.135791948256355	38.0	36.0	40.0	32.0	41.0
15	37.09235862950254	38.0	36.0	40.0	32.0	41.0
16	37.06901076403669	38.0	36.0	40.0	32.0	41.0
17	36.97786728062348	38.0	36.0	40.0	31.0	41.0
18	36.836626828050555	38.0	36.0	40.0	31.0	41.0
19	36.82828555682209	38.0	36.0	40.0	31.0	41.0
20	36.74775557583122	38.0	36.0	40.0	31.0	41.0
21	36.70128230110032	38.0	36.0	40.0	31.0	41.0
22	36.63342563314296	38.0	35.0	40.0	31.0	41.0
23	36.56541592200987	38.0	35.0	40.0	30.0	41.0
24	36.5177969142147	38.0	35.0	40.0	30.0	41.0
25	36.464426660957514	38.0	35.0	40.0	30.0	41.0
26	36.197305269284904	38.0	35.0	40.0	30.0	41.0
27	36.09003371131374	38.0	35.0	40.0	29.0	41.0
28	35.93766041308277	38.0	34.0	40.0	29.0	41.0
29	35.832868489079154	38.0	34.0	40.0	29.0	41.0
30	35.754024491925925	38.0	34.0	40.0	28.0	41.0
31	35.6699636459735	38.0	34.0	40.0	28.0	41.0
32	35.54718287655939	38.0	34.0	40.0	27.0	41.0
33	35.46215777496966	38.0	34.0	40.0	27.0	41.0
34	35.33334309018424	38.0	34.0	40.0	27.0	41.0
35	35.15319705521514	38.0	33.0	40.0	26.0	41.0
36	35.06693924520444	38.0	33.0	40.0	26.0	41.0
37	34.95154970850711	38.0	33.0	40.0	26.0	41.0
38	34.82010569178443	38.0	33.0	40.0	25.0	41.0
39	34.72666070662459	38.0	33.0	40.0	25.0	41.0
40	34.55376958722667	37.0	33.0	40.0	25.0	41.0
41	34.37385604407643	37.0	33.0	40.0	24.0	40.0
42	34.328481669525424	37.0	33.0	40.0	24.0	40.0
43	33.04321086255301	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	2.0
12	2.0
13	10.0
14	13.0
15	21.0
16	82.0
17	125.0
18	272.0
19	454.0
20	827.0
21	1404.0
22	2343.0
23	3624.0
24	5635.0
25	7996.0
26	11698.0
27	15599.0
28	21325.0
29	27556.0
30	36038.0
31	45472.0
32	57376.0
33	73779.0
34	95912.0
35	124305.0
36	168376.0
37	218057.0
38	213572.0
39	63859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57485525711672	17.951295472849054	11.764169665504486	27.70967960452974
2	20.906283216850472	19.41925550767265	32.38050714995973	27.29395412551715
3	21.390752679719103	21.562696269509868	28.24407626735221	28.80247478341882
4	16.54229469425235	14.997060400203724	32.021984694009824	36.4386602115341
5	16.14270983432031	35.701460433321266	32.565915194009406	15.589914538349023
6	37.89992983430358	32.66167171653393	14.614870611612382	14.823527837550104
7	32.27881288673718	28.61531050620494	19.621473212008286	19.484403395049597
8	29.54552867217901	32.058781960307456	18.272351621295915	20.12333774621762
9	28.20226119201399	13.113793037120915	17.26611364835696	41.41783212250814
10	18.955526322171774	25.125006167723612	30.63405871338358	25.285408796721033
11	38.66589838434912	20.256644206395865	19.454380170956753	21.62307723829826
12	24.556488403425156	23.052065622906635	26.760142873749416	25.631303099918796
13	31.51811303618426	17.372240309565363	23.445796372291326	27.66385028195905
14	23.916132339695636	18.645676613915555	23.19181160468697	34.24637944170184
15	27.450509767583448	25.22711858169955	19.96837107701417	27.354000573702837
16	27.026755794105917	23.919561175873373	20.8731656771826	28.180517352838113
17	25.82248162436514	24.912920105608155	23.18545571323556	26.079142556791147
18	26.015165491523668	22.539161908808012	24.129640114372595	27.31603248529573
19	27.054688264431846	22.82133003719033	23.16764249114148	26.95633920723635
20	26.348682532421318	23.481171926027457	23.278117920185057	26.892027621366164
21	29.136075454467147	22.673221040342348	22.1050377966466	26.08566570854391
22	28.217481879437102	23.217318800643284	22.376166745139624	26.18903257477999
23	27.090398338770687	23.323779982454397	22.47050155510265	27.115320123672266
24	27.291027070243473	23.21146469009593	22.71478522522854	26.78272301443205
25	27.203048151731856	22.744473928718676	23.540382072706382	26.51209584684309
26	27.88371394808742	23.374292593462968	23.55125399229432	25.1907394661553
27	28.505922269120155	22.733518378980065	22.085635601689663	26.674923750210123
28	26.376280482144544	22.171607396585046	24.324749255900734	27.127362865369676
29	26.55575078549619	22.458124292802538	24.315717199627677	26.67040772207359
30	26.079811597996557	23.27995778349994	23.981280227072585	26.65895039143092
31	26.599740244752002	23.1689805735523	23.337495327165332	26.893783854530373
32	25.78267367264316	23.116628099228848	23.564467556101196	27.5362306720268
33	27.054688264431846	23.193567837851173	23.232790378518427	26.518953519198558
34	27.701149329160746	23.269253124213353	22.86925011352793	26.160347433097968
35	27.970772934941596	22.99879321692574	23.884603772890618	25.14583007524205
36	27.183060545720185	23.97743324014147	21.863932072246413	26.975574141891933
37	26.124135577855075	23.13527762282969	23.636724006285643	27.10386279302959
38	27.310931046104464	23.083761450013004	23.769946836313217	25.83536066756931
39	25.781001069629628	23.105170768586174	23.99641728434502	27.11741087743918
40	26.651841828623425	22.302070431640296	25.094397532576036	25.951690207160244
41	25.031507659267348	22.362451400428686	25.751479626440844	26.85456131386312
42	25.50267992817843	23.059257815864807	24.583835462696353	26.854226793260416
43	25.686917150118628	20.561057954858118	26.155329624057384	27.59669527096587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	33.0
2	35.0
3	64.5
4	94.0
5	94.0
6	154.5
7	215.0
8	231.0
9	247.0
10	312.5
11	378.0
12	378.0
13	728.5
14	1079.0
15	1870.0
16	2661.0
17	2472.5
18	2284.0
19	2284.0
20	2841.5
21	3399.0
22	3278.5
23	3158.0
24	3828.0
25	4498.0
26	4498.0
27	6162.0
28	7826.0
29	10291.0
30	12756.0
31	16072.0
32	19388.0
33	19388.0
34	24338.5
35	29289.0
36	34330.5
37	39372.0
38	43458.0
39	47544.0
40	47544.0
41	52657.5
42	57771.0
43	60935.0
44	64099.0
45	67900.5
46	71702.0
47	71702.0
48	74467.5
49	77233.0
50	81920.5
51	86608.0
52	93742.0
53	100876.0
54	100876.0
55	102217.5
56	103559.0
57	98158.0
58	92757.0
59	83293.5
60	73830.0
61	73830.0
62	72972.0
63	72114.0
64	63115.0
65	54116.0
66	45565.0
67	37014.0
68	37014.0
69	31766.5
70	26519.0
71	25178.0
72	23837.0
73	27170.5
74	30504.0
75	30504.0
76	29042.5
77	27581.0
78	21213.0
79	14845.0
80	9290.5
81	3736.0
82	3736.0
83	2485.5
84	1235.0
85	955.0
86	675.0
87	559.5
88	444.0
89	444.0
90	390.5
91	337.0
92	202.5
93	68.0
94	45.0
95	22.0
96	22.0
97	13.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1195741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.776248213922685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.92885886107774	33.40751484802745
2	9.694630513547677	7.906213203253776
3	2.8344356489045355	3.4673295469836702
4	1.2704857534768026	2.072225697440908
5	0.7134806202757651	1.4546531434094059
6	0.495178819288452	1.211492066734985
7	0.3731778210497232	1.0651754021338036
8	0.2849969973405621	0.9296886643025141
9	0.2427916421993626	0.8910119039928406
>10	1.712306049423078	14.277264633136225
>50	0.24045615396353875	6.846920302357681
>100	0.18023162721220196	14.164068833706917
>500	0.02007171962380167	5.745346186193974
>1k	0.008690847672161547	6.015687623962955
>5k	2.0692494457527494E-4	0.5454079443628891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	6464	0.5405852939725241	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4343	0.3632057443877897	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3111	0.26017339875441253	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2884	0.2411893545508601	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2804	0.23449894249674472	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2225	0.1860770852550845	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2221	0.18574256465237873	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2170	0.18147742696788016	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2162	0.18080838576246863	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2148	0.17963756365299843	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2103	0.17587420687255853	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2060	0.17227811039347152	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2045	0.17102365813332487	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1997	0.1670094109008556	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1969	0.1646677666819152	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1936	0.16190797170959262	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1900	0.15889728628524072	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1790	0.14969796971083205	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1621	0.13556447424651327	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1556	0.1301285144525445	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	1523	0.1273687194802219	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.11674769034431369	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	1392	0.11641316974160792	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	1383	0.11566049838551995	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	1373	0.11482419687875552	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1371	0.11465693657740264	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	1355	0.11331885416657955	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1344	0.11239892250913869	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1301	0.10880282603005166	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	1300	0.10871919587937523	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1246	0.10420316774284732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.363015067644247E-5	0.0	0.0	0.0	0.0
6	8.363015067644247E-5	0.0	0.0	0.0	0.0
7	8.363015067644247E-5	0.0	0.0	0.0	0.0
8	8.363015067644247E-5	0.0	0.0	0.0	8.363015067644247E-5
9	8.363015067644247E-5	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
10	1.6726030135288494E-4	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
11	1.6726030135288494E-4	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
12	1.6726030135288494E-4	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
13	2.508904520293274E-4	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
14	2.508904520293274E-4	0.0	0.0	8.363015067644247E-5	8.363015067644247E-5
15	2.508904520293274E-4	0.0	0.0	1.6726030135288494E-4	8.363015067644247E-5
16	2.508904520293274E-4	0.0	0.0	3.345206027057699E-4	1.6726030135288494E-4
17	2.508904520293274E-4	0.0	0.0	6.690412054115398E-4	1.6726030135288494E-4
18	2.508904520293274E-4	0.0	0.0	7.526713560879822E-4	1.6726030135288494E-4
19	2.508904520293274E-4	0.0	0.0	9.199316574408672E-4	1.6726030135288494E-4
20	2.508904520293274E-4	0.0	0.0	0.0010871919587937522	1.6726030135288494E-4
21	2.508904520293274E-4	0.0	0.0	0.0014217125614995221	1.6726030135288494E-4
22	2.508904520293274E-4	0.0	0.0	0.002090753766911062	1.6726030135288494E-4
23	2.508904520293274E-4	0.0	0.0	0.0028434251229990442	1.6726030135288494E-4
24	3.345206027057699E-4	0.0	0.0	0.003512466328410584	1.6726030135288494E-4
25	3.345206027057699E-4	0.0	0.0	0.004599658287204336	1.6726030135288494E-4
26	3.345206027057699E-4	0.0	0.0	0.006272261300733185	1.6726030135288494E-4
27	3.345206027057699E-4	0.0	0.0	0.01254452260146637	1.6726030135288494E-4
28	4.1815075338221235E-4	0.0	0.0	0.030357744695548618	1.6726030135288494E-4
29	4.1815075338221235E-4	0.0	0.0	0.057035762761333764	1.6726030135288494E-4
30	4.1815075338221235E-4	0.0	0.0	0.09040419288123432	1.6726030135288494E-4
31	4.1815075338221235E-4	0.0	0.0	0.18256461892667392	1.6726030135288494E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	40	0.001931233	23.125	35
GGTTCGC	70	5.102207E-6	21.142857	23
CCAGGAC	1475	0.0	20.945763	3
CAGGACA	1485	0.0	20.929293	4
TGCATCA	1395	0.0	20.82079	14
CGTAGCG	45	0.0038258967	20.555555	15
GGTATCA	770	0.0	20.422077	1
GACAGGC	1490	0.0	20.114094	7
ACAGGCT	1480	0.0	19.875	8
GCATCAG	1465	0.0	19.825937	15
ATTAGAC	75	9.266287E-6	19.733334	3
CGGTTCG	75	9.266287E-6	19.733334	22
TTAGACT	85	1.2453929E-6	19.588236	4
TCCAGGA	1560	0.0	19.448719	2
GCTGCAT	1530	0.0	18.862745	12
CTGCATC	1550	0.0	18.500002	13
TTACAAC	60	9.2371134E-4	18.5	3
TACCGTC	80	1.6167598E-5	18.5	7
GCGACTT	50	0.007035045	18.499998	13
GATCTAG	50	0.007035045	18.499998	1
>>END_MODULE