FastQCFastQC Report
Fri 10 Feb 2017
ERR1630268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790857
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC241190.8642148272018236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117420.4207309797671468No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT106330.3809940817462163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88230.3161394510718392No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG76500.274109350640323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75390.27013207770946346No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT74170.2657606606142844No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT73740.26421991524467214No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG70650.25314804735606306No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT66820.2394246641802142No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG64880.23247339437312628No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG61120.21900083021093522No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT60190.21566852045805285No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG58390.20921888867828056No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT56450.2022676188711926No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC50600.1813063155869326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.18127048429926723No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG50460.180804677559617No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG49300.1766482481904304No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG46120.16525389871283266No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT45630.16349816561722796No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT45560.16324734660357015No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG44080.1579443160290907No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA42690.15296376704359987No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC42620.15271294802994206No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG41790.14973895115371372No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA37720.1351556170738952No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT37620.1347973041972412No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA36920.13228911406066307No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC34300.12290131669232783No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC33850.12128890874738477No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA33410.1197123320901071No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG32110.11505426469360486No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG31100.1114353046393993No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG31060.11129197948873767No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT31050.1112561482010723No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC30640.10978706540679083No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCC30310.10860463291383256No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT30180.10813882617418236No Hit
GGGCTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACC29930.1072430439825473No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA29640.10620393664025066No Hit
GTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGG29310.1050215041472924No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC28920.10362408392834171No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA28320.10147420666841764No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG28300.10140254409308683No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC28210.10108006250409819No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT28120.10075758091510958No Hit
GCCTGCACCAGGGCCCCCGCCCAGCTCCACCTGCCCCACCTGC28030.10043509932612098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGACA52200.019.9176254
TCCAGGA53600.019.5354482
CCAGGAC53650.019.482763
GACAGGC54450.018.7208447
TGCATCA53600.018.7070914
ACAGGCT55000.018.4663648
GCTGCAT54500.018.2963312
GGACAGG56150.018.154056
GCATCAG55450.018.01623215
CTGCATC56300.017.80994613
TATACTG2550.017.4117645
GGTATCA49150.017.2390631
GGCCATC60200.016.50249126
ATCAAGC60750.016.3835430
AGGCCAT60600.016.36303525
ATCAGAA62100.016.29549217
CATCAGA62400.016.21714816
CATCAAG62050.016.07010529
AGAGGCC62900.015.88235323
CCATCAA63500.015.58661428
Produced by FastQC (version 0.11.5)