FastQCFastQC Report
Fri 10 Feb 2017
ERR1630267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418543
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC184780.7640137057724423No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT85350.35289841859334314No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG64250.265655810130314No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG61320.2535410782442156No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG59950.24787651077528908No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT59880.2475870803206724No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT54780.2265000043414568No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG52440.2168247577156991No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT50790.2100024684283058No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG46470.19214047465767614No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC45130.18659994881215675No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG44970.1859383934873186No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT41940.17341018952319642No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG41720.17250055095154398No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT41670.17229381491253204No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT39790.16452053984568393No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC38900.16084063835127183No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT38380.15869058354554788No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG37260.15405969627168092No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG34490.14260651971042068No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG33190.13723138269611082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.128837899512227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30030.12416566503055766No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA29880.1235454569135219No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA29640.1225531239262647No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC29460.12180887418582179No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT29210.1207751939907622No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC28890.11945208334108594No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG27220.1125470996380879No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA27100.1120509331444593No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA26420.1092393230138972No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA26030.10762678190960426No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC25550.10564211593508985No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT25550.10564211593508985No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT25020.10345071392156352No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC24330.10059775658319906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA250.005497528729.621
TGCATCA34600.021.97543314
GACAGGC36150.021.3402487
CAGGACA38000.021.2754
GCATCAG36500.020.9835615
GCTGCAT36500.020.93287712
CCAGGAC38900.020.7827763
ACAGGCT37650.020.5391778
CTGCATC37450.020.30307213
GTCGTAC555.14535E-420.1818181
GGTATCA13750.019.9127271
TCCAGGA41350.019.596132
GGACAGG41200.019.128646
GGCCATC40450.018.79728126
ATCAAGC40550.018.6596830
CTAGTAC801.6178734E-518.53
AGGCCAT41100.018.36496425
AAGAGGC42500.017.89058922
CATCAGA42500.017.89058916
TCTAGCG3000.017.88333128
Produced by FastQC (version 0.11.5)