FastQCFastQC Report
Fri 10 Feb 2017
ERR1630266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574653
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT13430.23370625403504375No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13270.23092196508153617No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC13130.22848571224721703No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG12510.21769659255237508No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC12270.2135201591221137No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG11010.19159388361324137No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG10470.18219690839515323No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA10070.17523618601138427No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA9690.1686234997468037No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA9420.16392501213775967No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG9140.15905250646912136No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC8740.15209178408535237No Hit
GATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGG8660.15069963960859858No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT8560.14895945901265634No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8480.14756731453590255No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC8420.1465232061783372No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA8360.14547909782077184No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC8290.14426097140361227No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG7930.13799632125822017No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT7460.1298174724572916No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC7420.12912140021891472No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG7390.12859934604013204No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC7080.12320478619271108No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC7000.12181264171595729No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC6990.12163862365636305No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG6750.11746219022610166No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA6610.11502593739178252No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG6480.1127637026170576No Hit
GGATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAG6370.11084950396152111No Hit
GCTGAAGACTTGGAGGATTAGGGAAGAGAGATGGGGTGTGGGG6260.10893530530598466No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG6250.10876128724639042No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG6060.10545494411410017No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC6040.10510690799491172No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC5980.10406279963734637No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG5900.10267065516059257No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA5880.10232261904140412No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC5870.10214860098180989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3700.024.5000021
AAGACGG957.1268005E-921.4210535
CTTATAC2050.020.756137
GTATTAA450.003823515320.5555551
TCTTATA4550.020.3296737
CAAGACG1101.7498678E-920.1818184
GTTCAAA1153.0413503E-919.3043481
GTACTAT500.00703070718.51
GTGTAGA701.21785146E-418.51
GACGGAC1103.8417056E-818.57
CGCAAGA1351.1477823E-917.8148162
CGCGGTT852.7192806E-517.41176410
TCTAGCG752.0650007E-417.26666628
CTAGCGG752.0650007E-417.26666629
GCGCAAG1401.8626451E-917.1785721
TAATACT650.001578634617.0769234
ACGGACC1201.0384974E-716.9583328
GATGCCG1107.7908953E-716.81818221
ATAACGA1005.867507E-616.65000212
ATTGACG1005.867507E-616.65000232
Produced by FastQC (version 0.11.5)