##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574653
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.452090217922816	31.0	31.0	34.0	30.0	34.0
2	31.66721482355439	31.0	31.0	34.0	30.0	34.0
3	31.811865595411494	31.0	31.0	34.0	30.0	34.0
4	35.528525910418985	37.0	35.0	37.0	33.0	37.0
5	35.411998197172906	37.0	35.0	37.0	33.0	37.0
6	35.46583764463076	37.0	35.0	37.0	33.0	37.0
7	35.426864560004034	37.0	35.0	37.0	33.0	37.0
8	35.39885113277056	37.0	35.0	37.0	33.0	37.0
9	37.07632432093803	39.0	37.0	39.0	33.0	39.0
10	36.92365827725602	39.0	37.0	39.0	33.0	39.0
11	37.03191491212958	39.0	37.0	39.0	33.0	39.0
12	36.837042528273585	39.0	37.0	39.0	33.0	39.0
13	36.924537068456964	39.0	37.0	39.0	33.0	39.0
14	38.044613009938175	40.0	37.0	41.0	33.0	41.0
15	38.018249273909646	40.0	37.0	41.0	33.0	41.0
16	38.0048028984448	40.0	37.0	41.0	33.0	41.0
17	37.91475203296598	40.0	37.0	41.0	33.0	41.0
18	37.86170958822107	40.0	37.0	41.0	33.0	41.0
19	37.857611462917625	40.0	37.0	41.0	33.0	41.0
20	37.80130617955531	40.0	37.0	41.0	32.0	41.0
21	37.74696730026642	39.0	37.0	41.0	32.0	41.0
22	37.70206716052992	39.0	37.0	41.0	32.0	41.0
23	37.660381134354125	39.0	37.0	41.0	32.0	41.0
24	37.62016034024011	39.0	37.0	41.0	32.0	41.0
25	37.585407193558545	39.0	37.0	41.0	32.0	41.0
26	37.39135269458264	39.0	37.0	41.0	32.0	41.0
27	37.25396021599121	39.0	36.0	40.0	31.0	41.0
28	37.17606451197505	39.0	36.0	40.0	31.0	41.0
29	37.08523230540865	39.0	36.0	40.0	31.0	41.0
30	37.02314266174544	39.0	36.0	40.0	31.0	41.0
31	36.883578437770275	39.0	36.0	40.0	30.0	41.0
32	36.74972722669158	39.0	36.0	40.0	30.0	41.0
33	36.71522118565465	39.0	35.0	40.0	30.0	41.0
34	36.56096635708854	39.0	35.0	40.0	30.0	41.0
35	36.48863053007641	39.0	35.0	40.0	30.0	41.0
36	36.38464429838529	38.0	35.0	40.0	30.0	41.0
37	36.292024926346855	38.0	35.0	40.0	30.0	41.0
38	36.22507495827917	38.0	35.0	40.0	30.0	41.0
39	36.055598770040355	38.0	35.0	40.0	29.0	41.0
40	35.95000809183977	38.0	35.0	40.0	29.0	41.0
41	35.836123712918926	38.0	34.0	40.0	28.0	41.0
42	35.76860122543518	38.0	34.0	40.0	28.0	41.0
43	34.463951288864756	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	1.0
15	4.0
16	9.0
17	24.0
18	49.0
19	81.0
20	164.0
21	337.0
22	616.0
23	989.0
24	1552.0
25	2377.0
26	3455.0
27	5125.0
28	6900.0
29	9380.0
30	12127.0
31	15876.0
32	19927.0
33	25604.0
34	33063.0
35	44080.0
36	60881.0
37	87775.0
38	143699.0
39	100555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.634680407132656	18.714250164882113	12.0314346222851	25.619634805700137
2	20.34410331104162	20.615049429829828	33.25972369412498	25.78112356500358
3	21.397260607705867	20.660294125324327	28.53078292465192	29.411662342317886
4	15.028199626557246	15.246940327467184	33.85712769271195	35.86773235326362
5	16.461412365375278	34.97414961724728	32.953799945358334	15.610638072019114
6	37.60982714786141	33.47376590742587	14.93858032586622	13.977826618846503
7	32.79979396261744	28.56784877134549	19.351330281056565	19.2810269849805
8	29.000457667496732	31.833645695750306	19.331144186143636	19.834752450609326
9	27.960177707242455	12.759178147508148	18.38709621284497	40.893547932404424
10	17.807790092455793	25.515572006062786	32.40755725629206	24.26908064518936
11	39.0593975842813	20.162776492944438	19.30573754944288	21.472088373331385
12	22.89468601051417	24.246806333561295	27.81939709703073	25.0391105588938
13	32.6080260609446	17.706337563712363	23.5778809124811	26.107755462861938
14	24.838641754241255	19.628367031930573	22.882504746342576	32.65048646748559
15	26.811136459741792	26.305091942441788	21.532646658070178	25.351124939746246
16	28.56071403090213	24.021800982505965	21.554224897459857	25.86326008913205
17	26.039366365441406	24.241063737594686	23.178683483771945	26.54088641319196
18	25.52340281874453	22.730238944197627	25.109587873029465	26.63677036402838
19	28.008902763928837	23.767734615498394	23.518888790278655	24.70447383029411
20	28.043358339728496	23.141269600959188	23.511057977596913	25.3043140817154
21	27.6996726720299	23.5656996483095	22.66150181065791	26.073125869002684
22	28.51442522705006	22.426577430205707	23.34487073068443	25.7141266120598
23	25.70177132982861	23.020501067600797	23.92278470659685	27.354942895973743
24	26.300219436773148	24.202431728364772	23.510187887298944	25.987160947563137
25	26.593961921368198	23.813327347112086	23.515060392967584	26.077650338552132
26	27.65425395847581	23.8037563538344	22.163114087980052	26.37887559970974
27	27.499899939615734	23.222187998670503	22.37106566919515	26.906846392518613
28	26.78834009393495	23.394813913787974	23.38106648708003	26.43577950519705
29	26.497903952472186	24.094888567535538	24.196863150457755	25.210344329534518
30	26.91989774698818	23.611640416042377	23.017716778647287	26.450745058322152
31	26.12011074509313	23.252989195218678	23.633740709610844	26.99315935007735
32	25.141259159875613	24.06478344322574	23.542207210264284	27.25175018663437
33	25.752584603230122	22.6376613364935	24.31397730456467	27.29577675571171
34	27.165959283254416	23.093066598451585	24.36305039737024	25.377923720923757
35	27.65338386817784	22.837086032788484	23.3366918818835	26.172838217150172
36	25.57073573095416	23.731364841043202	22.776701766109287	27.92119766189335
37	25.622941148832425	23.190690729883947	25.361914059441087	25.824454061842538
38	25.469631238329914	22.197569663779706	24.271342879964084	28.061456217926295
39	25.10697760213555	22.983087184788037	26.166573567004786	25.743361646071627
40	26.341113680777788	22.132834945610654	25.403330357624515	26.12272101598704
41	24.632430353622098	22.54160336759749	26.312922755123523	26.513043523656886
42	24.512705928621273	23.335995809645123	26.342157789135356	25.809140472598248
43	24.638520985707896	21.216455843787468	25.833503000941437	28.3115201695632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	7.0
2	10.0
3	28.5
4	47.0
5	47.0
6	63.5
7	80.0
8	86.0
9	92.0
10	108.5
11	125.0
12	125.0
13	230.5
14	336.0
15	592.5
16	849.0
17	862.5
18	876.0
19	876.0
20	1139.5
21	1403.0
22	1652.0
23	1901.0
24	2446.0
25	2991.0
26	2991.0
27	3628.5
28	4266.0
29	5296.0
30	6326.0
31	7713.5
32	9101.0
33	9101.0
34	12888.0
35	16675.0
36	18654.5
37	20634.0
38	21680.5
39	22727.0
40	22727.0
41	23809.0
42	24891.0
43	27876.5
44	30862.0
45	31915.0
46	32968.0
47	32968.0
48	34630.0
49	36292.0
50	39049.0
51	41806.0
52	46945.5
53	52085.0
54	52085.0
55	52588.5
56	53092.0
57	49644.0
58	46196.0
59	46655.0
60	47114.0
61	47114.0
62	42298.5
63	37483.0
64	30702.5
65	23922.0
66	21178.0
67	18434.0
68	18434.0
69	16050.0
70	13666.0
71	13570.0
72	13474.0
73	11505.5
74	9537.0
75	9537.0
76	5677.5
77	1818.0
78	1404.0
79	990.0
80	824.5
81	659.0
82	659.0
83	514.5
84	370.0
85	307.5
86	245.0
87	197.5
88	150.0
89	150.0
90	140.0
91	130.0
92	74.5
93	19.0
94	11.5
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	574653.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.77831365672205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.84466630235013	49.5560566373929
2	6.721764495123263	7.498573766712066
3	1.5713959241811553	2.6294944421361333
4	0.6826417518285662	1.5230642299467192
5	0.40218653414818467	1.12166433251137
6	0.27320043069843325	0.914319558878926
7	0.19068128939262902	0.7445116537747173
8	0.14287490625587548	0.6375457067851985
9	0.10802093419600045	0.5422702994168982
>10	0.7983182721170909	9.027098746655307
>50	0.1286887166376929	5.082470164715259
>100	0.1202552317565069	14.203284402560692
>500	0.012806401303939693	4.844305567915803
>1k	0.0024988100105248177	1.675340490598035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1343	0.23370625403504375	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1327	0.23092196508153617	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1313	0.22848571224721703	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1251	0.21769659255237508	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1227	0.2135201591221137	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1101	0.19159388361324137	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	1047	0.18219690839515323	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1007	0.17523618601138427	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	969	0.1686234997468037	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	942	0.16392501213775967	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	914	0.15905250646912136	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	874	0.15209178408535237	No Hit
GATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGG	866	0.15069963960859858	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	856	0.14895945901265634	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	848	0.14756731453590255	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	842	0.1465232061783372	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	836	0.14547909782077184	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	829	0.14426097140361227	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	793	0.13799632125822017	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	746	0.1298174724572916	No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC	742	0.12912140021891472	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	739	0.12859934604013204	No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC	708	0.12320478619271108	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	700	0.12181264171595729	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	699	0.12163862365636305	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	675	0.11746219022610166	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	661	0.11502593739178252	No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG	648	0.1127637026170576	No Hit
GGATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAG	637	0.11084950396152111	No Hit
GCTGAAGACTTGGAGGATTAGGGAAGAGAGATGGGGTGTGGGG	626	0.10893530530598466	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	625	0.10876128724639042	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	606	0.10545494411410017	No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC	604	0.10510690799491172	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	598	0.10406279963734637	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	590	0.10267065516059257	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	588	0.10232261904140412	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	587	0.10214860098180989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	1.740180595942247E-4	0.0
15	0.0	0.0	0.0	3.480361191884494E-4	0.0
16	0.0	0.0	0.0	6.960722383768988E-4	0.0
17	0.0	0.0	0.0	0.0013921444767537976	0.0
18	0.0	0.0	0.0	0.0013921444767537976	0.0
19	0.0	0.0	0.0	0.0015661625363480223	0.0
20	0.0	0.0	0.0	0.0019141986555364717	0.0
21	0.0	0.0	0.0	0.0033063431322902692	0.0
22	0.0	0.0	0.0	0.005046523728232516	0.0
23	0.0	0.0	0.0	0.0074827765625516615	0.0
24	0.0	0.0	0.0	0.010441083575653482	0.0
25	0.0	0.0	0.0	0.012007246112001503	0.0
26	0.0	0.0	0.0	0.015139571184697548	0.0
27	0.0	0.0	0.0	0.022100293568466534	0.0
28	0.0	0.0	0.0	0.050291219222730936	0.0
29	0.0	0.0	0.0	0.0951878785980409	0.0
30	0.0	0.0	0.0	0.15679027169439644	0.0
31	0.0	0.0	0.0	0.3939768869213247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	370	0.0	24.500002	1
AAGACGG	95	7.1268005E-9	21.421053	5
CTTATAC	205	0.0	20.7561	37
GTATTAA	45	0.0038235153	20.555555	1
TCTTATA	455	0.0	20.32967	37
CAAGACG	110	1.7498678E-9	20.181818	4
GTTCAAA	115	3.0413503E-9	19.304348	1
GTACTAT	50	0.007030707	18.5	1
GTGTAGA	70	1.21785146E-4	18.5	1
GACGGAC	110	3.8417056E-8	18.5	7
CGCAAGA	135	1.1477823E-9	17.814816	2
CGCGGTT	85	2.7192806E-5	17.411764	10
TCTAGCG	75	2.0650007E-4	17.266666	28
CTAGCGG	75	2.0650007E-4	17.266666	29
GCGCAAG	140	1.8626451E-9	17.178572	1
TAATACT	65	0.0015786346	17.076923	4
ACGGACC	120	1.0384974E-7	16.958332	8
GATGCCG	110	7.7908953E-7	16.818182	21
ATAACGA	100	5.867507E-6	16.650002	12
ATTGACG	100	5.867507E-6	16.650002	32
>>END_MODULE