FastQCFastQC Report
Fri 10 Feb 2017
ERR1630265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802565
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC107710.3843265009018524No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT65490.23367879067925276No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG44800.15985356271843829No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT42540.1517895213848742No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG41780.14907771987447213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41270.14725795833459707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.14643728156171223No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG39840.14215548970318262No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG38650.1379093794434741No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT37860.1350905331366088No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT37010.132057597236817No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG33940.12110334639874544No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT32440.11575110657558343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31070.11086272753709549No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC31040.11075568274063224No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG30620.1092570555901469No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC28900.10311982059292113No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT28660.10226346222121521No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG28550.10187096463418334No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT28400.10133574065186712No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG28040.10005120309430826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.021.0597481
CAGGACA30550.018.4091644
GACAGGC28000.018.3017867
TGCATCA27750.018.214
CCAGGAC31650.018.1200663
ACAGGCT29650.017.6576738
GCATCAG29100.017.48281715
CGCTATA650.001580700917.0769232
GGACAGG32200.016.9487576
GCTGCAT30050.016.86855112
CTGCATC30250.016.81818213
TTAGACT2550.016.6862744
TCCAGGA34500.016.6231882
ATCAAGC30750.016.48455230
AGGCCAT32300.015.75077425
GGCGTAA1659.749783E-1015.6969713
CATCAGA33300.015.55555516
GGCCATC32900.015.40729526
ATCAGAA33650.015.33878217
GTCTAAC855.367569E-415.2352951
Produced by FastQC (version 0.11.5)