FastQCFastQC Report
Fri 10 Feb 2017
ERR1630264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559759
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97660.38152029155869754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.32776523102370186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64830.25326603012236704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.17818083655531636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36400.020.2280221
GCGCGTT701.2199394E-418.525
TATACTG2850.017.5263165
TCTGTCG1801.0913936E-1116.4444458
ACCGAAC700.002593659315.8571428
GACGGAC2700.015.7592597
CGAATTA1659.749783E-1015.69696915
CGCGTTC855.3673435E-415.23529426
ATAGCGA1252.9619969E-614.7999998
TTAACGG1203.3034696E-513.87500135
CGGACCA3200.013.8759
GTATCAA53700.013.8147132
GTTCTAG3400.013.60294151
TAACGGC1254.8489223E-513.3236
ACGAACG2102.044544E-913.21428515
AATACGC1551.8898972E-613.1290325
GTGCGTA850.0094088513.05882415
TGCGTAC850.0094088513.05882416
GGACCGT1000.00183354812.9500016
CGATATG1000.00183354812.95000134
Produced by FastQC (version 0.11.5)