FastQCFastQC Report
Fri 10 Feb 2017
ERR1630263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033144
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.4167860433782706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32330.31292830428284923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27100.26230612576756No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22690.21962088537512678No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT22230.2151684566720612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.13463757230357046No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT12520.12118349426604616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA400.001931055423.12500215
TCGCGTA652.682409E-622.769239
GTCGCGT705.1010993E-621.1428578
TATACTG1500.020.9666675
CGTAAGT450.003825550720.55555520
ACCGTAT555.142609E-420.18181811
CGATCGC555.142609E-420.18181819
CGGGTTA656.901266E-519.9230779
CGTTATT656.901266E-519.9230772
CGCGTAA851.2450582E-619.58823610
GGTATCA11650.019.5321881
ATTAGAC701.219046E-418.53
CCGATCG609.2359504E-418.518
CGGTTGC801.6164122E-518.531
TACGGGT609.2359504E-418.54
AACGGGT902.151788E-618.57
GCATTCG500.007034412618.522
CTAGTAC500.007034412618.53
ACAGGCT7650.018.3790868
GACAGGC7400.018.07
Produced by FastQC (version 0.11.5)