##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033144
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.884816637370978	31.0	30.0	31.0	26.0	34.0
2	30.160819788916164	31.0	30.0	31.0	26.0	34.0
3	30.267917153852707	31.0	30.0	31.0	27.0	34.0
4	34.059802892917155	35.0	33.0	37.0	30.0	37.0
5	32.84615890911625	35.0	32.0	37.0	26.0	37.0
6	33.09035816885158	35.0	32.0	37.0	27.0	37.0
7	33.21211563925261	35.0	32.0	37.0	28.0	37.0
8	33.17104198446683	35.0	32.0	37.0	27.0	37.0
9	34.652414377860204	37.0	33.0	39.0	28.0	39.0
10	34.07954554253811	35.0	32.0	38.0	27.0	39.0
11	34.42847754040095	37.0	33.0	39.0	27.0	39.0
12	34.197107082846145	37.0	32.0	39.0	27.0	39.0
13	34.402817032282044	37.0	33.0	39.0	27.0	39.0
14	35.09788954879475	37.0	33.0	39.0	27.0	40.0
15	35.06250532355606	37.0	33.0	39.0	27.0	40.0
16	34.99881720263584	37.0	33.0	39.0	27.0	40.0
17	34.881810280077126	37.0	33.0	39.0	27.0	40.0
18	34.730448030477845	37.0	33.0	39.0	27.0	40.0
19	34.78187358199825	37.0	33.0	39.0	27.0	40.0
20	34.731583399797124	37.0	33.0	39.0	27.0	40.0
21	34.66601074003237	37.0	32.0	39.0	27.0	40.0
22	34.55926279395709	37.0	32.0	39.0	26.0	40.0
23	34.482248360344734	37.0	32.0	39.0	26.0	40.0
24	34.391646275833764	37.0	32.0	39.0	25.0	40.0
25	34.35394775558876	37.0	32.0	39.0	25.0	40.0
26	34.020364053800826	36.0	32.0	39.0	25.0	40.0
27	33.89860464756123	36.0	32.0	39.0	24.0	40.0
28	33.75405267803907	36.0	31.0	39.0	24.0	40.0
29	33.63217905732405	36.0	31.0	39.0	24.0	40.0
30	33.49460772167287	36.0	31.0	39.0	24.0	40.0
31	33.40110865474706	36.0	31.0	39.0	24.0	40.0
32	33.253177679006996	36.0	31.0	39.0	23.0	40.0
33	33.10986077449029	36.0	30.0	38.0	23.0	40.0
34	32.99660453915427	36.0	30.0	38.0	22.0	40.0
35	32.78036653167419	35.0	30.0	38.0	21.0	40.0
36	32.659413402197565	35.0	30.0	38.0	21.0	40.0
37	32.552018886041054	35.0	30.0	38.0	20.0	40.0
38	32.41531480606769	35.0	30.0	38.0	19.0	40.0
39	32.28986472360097	35.0	30.0	38.0	19.0	40.0
40	32.135109916913805	35.0	30.0	38.0	19.0	40.0
41	31.96741983692496	35.0	29.0	38.0	18.0	40.0
42	31.900660508118907	35.0	29.0	38.0	18.0	40.0
43	30.612150871514523	34.0	27.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	9.0
12	12.0
13	24.0
14	51.0
15	126.0
16	229.0
17	384.0
18	731.0
19	1243.0
20	2109.0
21	3490.0
22	5543.0
23	8277.0
24	11900.0
25	16592.0
26	22450.0
27	30041.0
28	38423.0
29	48536.0
30	60144.0
31	72694.0
32	86975.0
33	100778.0
34	111266.0
35	115798.0
36	109151.0
37	90621.0
38	67074.0
39	28467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36039506593466	21.051470075807437	13.314794452661003	25.273340405596894
2	19.33680106548555	22.123150306249663	34.432760583229445	24.10728804503535
3	21.037628830056605	24.403955305359176	29.303659509226204	25.254756355358015
4	16.43265604794685	16.84595758190533	34.302091479987304	32.41929489016052
5	14.474748921737918	38.03777595378766	33.76479948584128	13.722675638633145
6	34.28970211316138	36.6229683374244	15.381979665951699	13.705349883462517
7	29.510503860062105	31.14648103265372	21.4766770169502	17.866338090333972
8	27.897659958340753	35.5897145025282	18.984091278660088	17.528534260470952
9	27.491714610935166	14.2583221699976	18.868424924308712	39.38153829475852
10	16.905387825898423	27.869977466839085	32.90586791386293	22.318766793399565
11	36.40925175967726	22.277823807717027	21.210789589834526	20.102134842771193
12	21.888236296198787	25.49692976003345	29.959424823645108	22.655409120122656
13	30.386761187211075	19.72706612050208	25.220782388515055	24.66539030377179
14	22.4356914428192	19.990049789767934	26.097426883377338	31.476831884035526
15	25.05681686192825	27.821291126890348	22.42756092083969	24.694331090341713
16	24.35855989097357	26.586322913359613	23.922802629643108	25.132314566023712
17	23.312045561896504	27.165912980184757	25.971113416909937	23.550928041008802
18	23.639976615070115	25.597302989709082	26.876989074127128	23.88573132109367
19	24.604701764710438	25.444178159094953	26.674790735850955	23.27632934034365
20	24.482066391519478	25.767850367422163	26.42593868812092	23.324144552937444
21	25.37632701733737	25.288149570631006	25.751202155749827	23.584321256281797
22	25.080434092440164	25.493832418326974	26.09345841431591	23.332275074916954
23	24.722884709198333	25.653151932354056	25.99976382769488	23.624199530752733
24	24.500456857901707	25.58965642737121	26.268167844947076	23.64171886978001
25	24.63490084634862	25.08004692472685	26.663078912523325	23.6219733164012
26	24.46493422020551	25.68373818170555	26.6784688291274	23.17285876896154
27	25.025165901365153	25.504189154658015	25.719647987115057	23.750996956861773
28	24.13748712667353	25.09030686912957	26.869923263359226	23.902282740837677
29	24.20107942358471	25.128249305033957	27.060796946021075	23.60987432536026
30	24.036145977714625	25.734941111790803	26.58012822994665	23.64878468054792
31	24.360495729540123	25.43778989182534	26.49088607202868	23.710828306605855
32	23.862210882510084	25.654216643565658	26.585258202148005	23.898314271776247
33	24.443446412116803	25.755944960237876	26.181248693308966	23.619359934336355
34	24.971059213430074	25.382715284606988	26.227708818906176	23.418516683056765
35	24.730821647321186	25.429078618275863	26.82520539247191	23.01489434193104
36	24.5887310965364	25.686641939555376	25.659056239982036	24.065570723926193
37	24.081347808243574	25.114795226996428	26.781745816652858	24.022111148107136
38	24.641095529761582	25.35396808189371	26.508211827199307	23.496724561145395
39	23.56902813160605	25.425110149214436	27.082381546038114	23.9234801731414
40	24.183560084557428	24.57034063015417	27.59925044330703	23.64684884198137
41	23.24216178964404	24.783476456331353	27.857394516156507	24.1169672378681
42	23.204896897237944	25.23849531139899	27.177721595440712	24.37888619592235
43	23.71857166087206	23.950388329216448	27.88101174666842	24.45002826324307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	354.0
1	282.0
2	210.0
3	326.0
4	442.0
5	442.0
6	654.0
7	866.0
8	825.5
9	785.0
10	1144.0
11	1503.0
12	1503.0
13	2704.5
14	3906.0
15	6463.5
16	9021.0
17	8435.5
18	7850.0
19	7850.0
20	9250.5
21	10651.0
22	10380.5
23	10110.0
24	11873.5
25	13637.0
26	13637.0
27	16023.0
28	18409.0
29	21609.5
30	24810.0
31	28102.5
32	31395.0
33	31395.0
34	35908.5
35	40422.0
36	44716.0
37	49010.0
38	52071.0
39	55132.0
40	55132.0
41	58784.0
42	62436.0
43	64324.5
44	66213.0
45	67557.0
46	68901.0
47	68901.0
48	69777.5
49	70654.0
50	71622.0
51	72590.0
52	73241.5
53	73893.0
54	73893.0
55	71845.0
56	69797.0
57	66056.5
58	62316.0
59	55647.5
60	48979.0
61	48979.0
62	45975.0
63	42971.0
64	37766.5
65	32562.0
66	27433.5
67	22305.0
68	22305.0
69	19092.5
70	15880.0
71	14065.5
72	12251.0
73	12371.5
74	12492.0
75	12492.0
76	11384.5
77	10277.0
78	8546.5
79	6816.0
80	4375.5
81	1935.0
82	1935.0
83	1324.5
84	714.0
85	534.0
86	354.0
87	263.0
88	172.0
89	172.0
90	132.5
91	93.0
92	54.0
93	15.0
94	13.0
95	11.0
96	11.0
97	7.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1033144.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.00778699087684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02605883425998	55.70345436518828
2	7.614786323709723	9.748112419781082
3	2.072780561760256	3.980222900279415
4	0.9284816418356757	2.377202206222301
5	0.5105386500629212	1.6339224581918634
6	0.3339337821053401	1.2824617436433892
7	0.22587102097909084	1.0120252938769063
8	0.16658807595053493	0.8530347264529481
9	0.13458477343277184	0.7753026159090253
>10	0.8549538811560428	10.707522432360769
>50	0.08218679304115455	3.6483366997103617
>100	0.0451199878516574	5.181575298317296
>500	0.0028962149349584586	1.3003940807941234
>1k	0.0012194589199825089	1.796432759272314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4306	0.4167860433782706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3233	0.31292830428284923	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2710	0.26230612576756	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2269	0.21962088537512678	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2223	0.2151684566720612	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.13463757230357046	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1252	0.12118349426604616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.679192832751291E-5	0.0	0.0	0.0	0.0
6	9.679192832751291E-5	0.0	0.0	0.0	0.0
7	9.679192832751291E-5	0.0	0.0	0.0	0.0
8	1.9358385665502583E-4	0.0	0.0	0.0	0.0
9	1.9358385665502583E-4	0.0	0.0	0.0	0.0
10	3.8716771331005165E-4	0.0	0.0	0.0	0.0
11	3.8716771331005165E-4	0.0	0.0	0.0	0.0
12	4.8395964163756454E-4	0.0	0.0	0.0	0.0
13	4.8395964163756454E-4	0.0	0.0	0.0	0.0
14	4.8395964163756454E-4	0.0	0.0	9.679192832751291E-5	0.0
15	4.8395964163756454E-4	0.0	0.0	1.9358385665502583E-4	9.679192832751291E-5
16	4.8395964163756454E-4	0.0	0.0	1.9358385665502583E-4	1.9358385665502583E-4
17	5.807515699650774E-4	0.0	0.0	2.903757849825387E-4	1.9358385665502583E-4
18	6.775434982925904E-4	0.0	0.0	3.8716771331005165E-4	2.903757849825387E-4
19	7.743354266201033E-4	0.0	0.0	4.8395964163756454E-4	2.903757849825387E-4
20	7.743354266201033E-4	0.0	0.0	5.807515699650774E-4	2.903757849825387E-4
21	7.743354266201033E-4	0.0	0.0	0.001064711211602642	2.903757849825387E-4
22	7.743354266201033E-4	0.0	0.0	0.0017422547098952324	2.903757849825387E-4
23	7.743354266201033E-4	0.0	0.0	0.002129422423205284	2.903757849825387E-4
24	7.743354266201033E-4	0.0	0.0	0.0023230062798603097	2.903757849825387E-4
25	7.743354266201033E-4	0.0	0.0	0.0028069659214978743	2.903757849825387E-4
26	7.743354266201033E-4	0.0	0.0	0.0037748852047730036	2.903757849825387E-4
27	7.743354266201033E-4	0.0	0.0	0.010840695972681447	3.8716771331005165E-4
28	7.743354266201033E-4	0.0	0.0	0.035135469982887184	3.8716771331005165E-4
29	7.743354266201033E-4	0.0	0.0	0.07056131575075691	3.8716771331005165E-4
30	7.743354266201033E-4	0.0	0.0	0.12457121175750911	3.8716771331005165E-4
31	8.711273549476162E-4	0.0	0.0	0.2858265643511456	3.8716771331005165E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	40	0.0019310554	23.125002	15
TCGCGTA	65	2.682409E-6	22.76923	9
GTCGCGT	70	5.1010993E-6	21.142857	8
TATACTG	150	0.0	20.966667	5
CGTAAGT	45	0.0038255507	20.555555	20
ACCGTAT	55	5.142609E-4	20.181818	11
CGATCGC	55	5.142609E-4	20.181818	19
CGGGTTA	65	6.901266E-5	19.923077	9
CGTTATT	65	6.901266E-5	19.923077	2
CGCGTAA	85	1.2450582E-6	19.588236	10
GGTATCA	1165	0.0	19.532188	1
ATTAGAC	70	1.219046E-4	18.5	3
CCGATCG	60	9.2359504E-4	18.5	18
CGGTTGC	80	1.6164122E-5	18.5	31
TACGGGT	60	9.2359504E-4	18.5	4
AACGGGT	90	2.151788E-6	18.5	7
GCATTCG	50	0.0070344126	18.5	22
CTAGTAC	50	0.0070344126	18.5	3
ACAGGCT	765	0.0	18.379086	8
GACAGGC	740	0.0	18.0	7
>>END_MODULE