FastQCFastQC Report
Fri 10 Feb 2017
ERR1630262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787949
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT279163.5428688912607287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188922.397617104660327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163342.0729768043363213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120001.5229412055856408No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA49650.6301169238110588No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42430.5384866279416561No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA41460.5261761865298389No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.44266824375689295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.4184280962346548No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32290.4097980960696695No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31240.39647236052079515No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA25340.32159441791283444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.26346882856631587No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA19580.2484932400447237No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA17760.22539529842667486No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA17110.21714603356308596No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.21371941585051824No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.17196544446404527No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12660.1606702971892851No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA12600.15990882658649228No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA12400.15737059124384953No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA12400.15737059124384953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11630.14759838517467502No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11190.142014267420861No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10570.1341457378586685No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9900.12564264946081535No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA9150.1161242669259051No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA9110.11561661985737656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT9090.11536279632311229No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9050.11485514925458373No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9040.1147282374874516No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8800.11168235507628033No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA8700.11041323740495895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACAC200.001841584837.05
TTGGGCG200.001841584837.020
CGATCGT352.3873621E-531.71428526
ATAAGGT303.5984605E-430.8333323
CTCTGGA303.5984605E-430.8333322
GCGAAGT250.00549530829.59999832
GTTACTA250.00549530829.59999835
CCCAACG250.00549530829.59999821
GTTGTGC250.00549530829.59999818
TATACTG250.00549530829.5999985
TGGCTTT250.00549530829.59999822
GGAGCCG405.9377955E-527.7521
ATCGTGC405.9377955E-527.7528
ACAGAGG405.9377955E-527.753
AGCCTTA405.9377955E-527.756
TACACTG659.3839844E-825.6153855
CCGATCG451.3226963E-424.66666625
ATTCACC451.3226963E-424.6666666
ATACACT851.9736035E-923.9411774
GATACAC952.8012437E-1023.3684223
Produced by FastQC (version 0.11.5)