##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630262.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787949
Sequences flagged as poor quality	0
Sequence length	43
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63379736505789	31.0	31.0	34.0	30.0	34.0
2	31.728105499213783	31.0	31.0	34.0	30.0	34.0
3	31.762581080755226	33.0	31.0	34.0	30.0	34.0
4	35.49168410645866	37.0	35.0	37.0	33.0	37.0
5	35.477782191487016	37.0	35.0	37.0	33.0	37.0
6	35.60554172922359	37.0	35.0	37.0	33.0	37.0
7	35.55808941949289	37.0	35.0	37.0	33.0	37.0
8	35.656652905200716	37.0	35.0	37.0	33.0	37.0
9	37.27674506852601	39.0	37.0	39.0	34.0	39.0
10	37.16669987524573	39.0	37.0	39.0	34.0	39.0
11	37.27648363028572	39.0	37.0	39.0	34.0	39.0
12	37.23918045457257	39.0	37.0	39.0	34.0	39.0
13	37.30177333812214	39.0	37.0	39.0	34.0	39.0
14	38.281703511267864	40.0	38.0	41.0	34.0	41.0
15	38.249140490057094	40.0	38.0	41.0	33.0	41.0
16	38.162774494288335	40.0	37.0	41.0	33.0	41.0
17	38.33203291075945	40.0	38.0	41.0	34.0	41.0
18	38.41165481522281	40.0	38.0	41.0	34.0	41.0
19	38.49905387277603	40.0	38.0	41.0	34.0	41.0
20	38.52093219231194	40.0	38.0	41.0	34.0	41.0
21	38.48812930786129	40.0	38.0	41.0	34.0	41.0
22	38.42301214926347	40.0	38.0	41.0	34.0	41.0
23	38.32256021646071	40.0	38.0	41.0	34.0	41.0
24	38.26805795806581	40.0	37.0	41.0	34.0	41.0
25	38.2075337363205	40.0	37.0	41.0	34.0	41.0
26	37.89059571114374	40.0	37.0	41.0	33.0	41.0
27	37.650861921266475	40.0	36.0	41.0	33.0	41.0
28	37.4943644829805	39.0	35.0	41.0	33.0	41.0
29	37.29267503353643	39.0	35.0	41.0	33.0	41.0
30	37.00167904267916	39.0	35.0	41.0	32.0	41.0
31	36.671757943724785	38.0	35.0	40.0	32.0	41.0
32	36.382622479373666	38.0	35.0	40.0	31.0	41.0
33	36.1241653964914	38.0	35.0	40.0	31.0	41.0
34	35.954849869725074	38.0	35.0	40.0	30.0	41.0
35	35.651387335982406	38.0	35.0	40.0	30.0	41.0
36	35.342160469776594	38.0	35.0	40.0	27.0	41.0
37	35.033931130060445	38.0	35.0	40.0	25.0	41.0
38	34.70692265616176	37.0	34.0	40.0	24.0	41.0
39	34.335639743181346	37.0	34.0	40.0	21.0	41.0
40	33.906024374674	37.0	33.0	40.0	18.0	41.0
41	33.4350256171402	37.0	33.0	40.0	15.0	41.0
42	33.011868788462195	36.0	33.0	40.0	12.0	41.0
43	31.927047308899432	35.0	31.0	40.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	19.0
11	24.0
12	15.0
13	15.0
14	3.0
15	6.0
16	13.0
17	15.0
18	50.0
19	102.0
20	246.0
21	428.0
22	848.0
23	1506.0
24	2504.0
25	4053.0
26	5968.0
27	8786.0
28	11893.0
29	15804.0
30	19695.0
31	23806.0
32	29433.0
33	36535.0
34	46005.0
35	57727.0
36	80079.0
37	132355.0
38	156098.0
39	153914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.69217297058566	20.168564209104904	14.785728517962458	17.35353430234698
2	17.16468959285436	28.022752741611452	33.07891754415578	21.733640121378414
3	20.84754216326184	30.951876327021168	30.826360589327486	17.37422092038952
4	13.256695547554473	17.765997545526425	34.119847858173564	34.85745904874554
5	10.942078738598564	44.04840922445488	31.35317133469298	13.656340702253573
6	27.57970376255316	36.25298084013052	18.515157706907427	17.6521576904089
7	23.57944486254821	34.92649905006542	24.27961708181621	17.214439005570156
8	30.974212798036422	31.216868096793064	20.51046450975888	17.298454595411634
9	28.123266861180102	12.402071707686666	23.66434883475961	35.81031259637362
10	19.031942422669488	31.86437193270123	29.01126849580366	20.09241714882562
11	27.950539946113263	28.618349664762565	20.521252009965114	22.909858379159058
12	18.900334920153462	34.99135096306995	28.963803494896247	17.14451062188035
13	29.3854043853092	21.767017916134165	28.5051443684807	20.34243333007593
14	22.305631455842953	24.355510318561226	31.956129140337765	21.382729085258056
15	24.370993554151347	28.916973052824485	28.285967746643504	18.426065646380664
16	18.012333285529902	28.015011123816393	31.738729283240414	22.233926307413295
17	17.489710628479763	31.301391333703073	31.900795609868148	19.308102427949017
18	15.732997947836724	27.075610223504317	35.51841553196971	21.672976296689253
19	17.274975918492185	28.805417609515338	38.518355883439156	15.401250588553319
20	17.551135923771717	28.17441230333435	38.42939073467953	15.845061038214403
21	19.091844776755856	27.910308915932376	36.60719158219631	16.390654725115457
22	19.169007131172194	28.53979128090777	35.653449652198304	16.637751935721727
23	17.40214150915859	29.87541071820638	36.14066392621857	16.581783846416457
24	15.825516626076052	31.592907662805587	35.36091802895873	17.220657682159633
25	16.726463260947092	30.173018812131243	36.32506672386157	16.775451203060097
26	17.032447531502672	30.274548225836952	36.268717899254895	16.424286343405477
27	15.668907505434996	31.631996487082287	35.73898818324536	16.960107824237355
28	15.959408540400458	31.703067076676284	37.6801036615314	14.657420721391867
29	16.897794146575475	31.511049573005362	36.518860992272344	15.072295288146822
30	15.774625007456066	32.9771343069158	36.0286008358409	15.219639849787233
31	16.178585162237656	32.36034311865362	36.2576765755144	15.203395143594319
32	14.400043657647894	33.13894681000928	36.31047187064137	16.150537661701456
33	14.093551740023782	33.378175491053355	36.85060835155575	15.677664417367113
34	15.106307641738233	32.76811062644918	35.59760847466016	16.527973257152432
35	14.823675136334966	33.66004652585383	35.52209597321654	15.994182364594664
36	15.185500584428688	33.49429975797926	34.00930770900147	17.31089194859058
37	14.865936754789969	33.018253719466614	34.94248993272407	17.173319593019347
38	14.813522194964396	33.068130043949544	35.11864346550348	16.999704295582582
39	14.39382498105842	32.974342248038894	35.81285083171626	16.81898193918642
40	14.691559986750411	32.97510371864169	36.282678193639434	16.050658100968462
41	14.160688064836682	33.01685769002816	35.69672656479036	17.125727680344795
42	13.645680113814473	33.40634990335669	35.439222589279254	17.50874739354958
43	13.974254678919577	32.834612392426415	35.32081391054497	17.87031901810904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	400.0
2	611.0
3	2301.0
4	3991.0
5	3991.0
6	5776.5
7	7562.0
8	7239.5
9	6917.0
10	9449.0
11	11981.0
12	11981.0
13	21933.0
14	31885.0
15	47216.5
16	62548.0
17	50089.5
18	37631.0
19	37631.0
20	38433.0
21	39235.0
22	26551.5
23	13868.0
24	14517.5
25	15167.0
26	15167.0
27	15522.5
28	15878.0
29	17881.5
30	19885.0
31	21657.0
32	23429.0
33	23429.0
34	26898.0
35	30367.0
36	32612.5
37	34858.0
38	37203.0
39	39548.0
40	39548.0
41	44455.0
42	49362.0
43	52596.5
44	55831.0
45	59772.5
46	63714.0
47	63714.0
48	64126.0
49	64538.0
50	55806.0
51	47074.0
52	40954.5
53	34835.0
54	34835.0
55	31173.5
56	27512.0
57	22570.5
58	17629.0
59	15284.0
60	12939.0
61	12939.0
62	10758.0
63	8577.0
64	6548.0
65	4519.0
66	3559.5
67	2600.0
68	2600.0
69	1937.0
70	1274.0
71	953.0
72	632.0
73	541.0
74	450.0
75	450.0
76	373.0
77	296.0
78	266.0
79	236.0
80	180.0
81	124.0
82	124.0
83	122.5
84	121.0
85	89.5
86	58.0
87	56.5
88	55.0
89	55.0
90	37.5
91	20.0
92	11.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	787949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.464221481038635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.70737783157963	15.659219744710477
2	11.374215683769682	5.110258005866109
3	4.807440880892484	3.239862501161047
4	2.673490033872857	2.402314889930774
5	1.7709276831263803	1.989125585032681
6	1.248430395604357	1.6827010146310173
7	0.9582269973233203	1.506807644788718
8	0.7366146400576351	1.3237979536344215
9	0.617908499964783	1.2492750058402715
>10	5.033918364903222	23.10278224399247
>50	0.6745529405510908	10.50055272679108
>100	0.3662345510567766	13.973823267836902
>500	0.016466359659917086	2.56113842062652
>1k	0.01192391561580203	6.113746981376051
>5k	0.0	0.0
>10k+	0.0022712220220575292	9.58459401378146
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27916	3.5428688912607287	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18892	2.397617104660327	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16334	2.0729768043363213	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12000	1.5229412055856408	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4965	0.6301169238110588	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4243	0.5384866279416561	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	4146	0.5261761865298389	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3488	0.44266824375689295	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3297	0.4184280962346548	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3229	0.4097980960696695	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3124	0.39647236052079515	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	2534	0.32159441791283444	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2076	0.26346882856631587	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1958	0.2484932400447237	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1776	0.22539529842667486	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1711	0.21714603356308596	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1684	0.21371941585051824	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1355	0.17196544446404527	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1266	0.1606702971892851	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	1260	0.15990882658649228	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1240	0.15737059124384953	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	1240	0.15737059124384953	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1163	0.14759838517467502	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1119	0.142014267420861	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1057	0.1341457378586685	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	990	0.12564264946081535	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	915	0.1161242669259051	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	911	0.11561661985737656	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	909	0.11536279632311229	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	905	0.11485514925458373	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	904	0.1147282374874516	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	880	0.11168235507628033	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	870	0.11041323740495895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.5382353426427347E-4	0.0
10	0.0	0.0	0.0	6.345588356606836E-4	0.0
11	0.0	0.0	0.0	0.0011422059041892305	0.0
12	0.0	0.0	0.0	0.001903676506982051	0.0
13	0.0	0.0	0.0	0.0025382353426427343	0.0
14	0.0	0.0	0.0	0.0040611765482283756	0.0
15	0.0	0.0	0.0	0.0060917648223425625	0.0
16	0.0	0.0	0.0	0.012945000247477946	0.0
17	0.0	0.0	0.0	0.02132117687819897	0.0
18	0.0	0.0	0.0	0.026016912262088028	0.0
19	0.0	0.0	0.0	0.032489412385827005	0.0
20	0.0	0.0	0.0	0.03946955957809452	0.0
21	0.0	0.0	0.0	0.05254147159270461	0.0
22	0.0	0.0	0.0	0.07208588373105367	0.0
23	0.0	0.0	0.0	0.10051411956865229	0.0
24	0.0	0.0	0.0	0.1396029438453504	0.0
25	0.0	0.0	0.0	0.15381706176414972	0.0
26	0.0	0.0	0.0	0.17539206217661296	0.0
27	0.0	0.0	0.0	0.20724691572677928	0.0
28	0.0	0.0	0.0	0.2642302991691087	0.0
29	0.0	0.0	0.0	0.34761133017492246	0.0
30	0.0	0.0	0.0	0.5685647167519725	0.0
31	0.0	0.0	0.0	1.0395342845793318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACAC	20	0.0018415848	37.0	5
TTGGGCG	20	0.0018415848	37.0	20
CGATCGT	35	2.3873621E-5	31.714285	26
ATAAGGT	30	3.5984605E-4	30.833332	3
CTCTGGA	30	3.5984605E-4	30.833332	2
GCGAAGT	25	0.005495308	29.599998	32
GTTACTA	25	0.005495308	29.599998	35
CCCAACG	25	0.005495308	29.599998	21
GTTGTGC	25	0.005495308	29.599998	18
TATACTG	25	0.005495308	29.599998	5
TGGCTTT	25	0.005495308	29.599998	22
GGAGCCG	40	5.9377955E-5	27.75	21
ATCGTGC	40	5.9377955E-5	27.75	28
ACAGAGG	40	5.9377955E-5	27.75	3
AGCCTTA	40	5.9377955E-5	27.75	6
TACACTG	65	9.3839844E-8	25.615385	5
CCGATCG	45	1.3226963E-4	24.666666	25
ATTCACC	45	1.3226963E-4	24.666666	6
ATACACT	85	1.9736035E-9	23.941177	4
GATACAC	95	2.8012437E-10	23.368422	3
>>END_MODULE