FastQCFastQC Report
Fri 10 Feb 2017
ERR1630261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160015
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG36340.16823957241037676No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG32910.15236005305518713No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT32730.15152672550885063No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC30700.14212864262516695No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG28910.13384166313659857No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA24650.11411957787330179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23340.10805480517496406No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT21790.100878929081511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.022.164251
CTTATAC7950.018.15094237
CGACGGT1301.394983E-817.0769257
CGCGCAA1504.6766218E-916.03333321
ACGGTAT1403.477544E-815.8571439
CGAATTA2100.015.85714215
ATAACGC1659.749783E-1015.696973
TAACGCC1752.240995E-914.84
TACGACG1508.111056E-814.85
TAACACG1001.0940709E-414.84
GCGGAAT2006.184564E-1114.827
TCTATAC2251.8189894E-1214.83
GACGGTA1651.6472768E-814.5757578
GTATCAA16100.014.2484472
CGCGAAC1304.4480294E-614.2307728
GTATTAT2750.014.1272741
TCTTATA13450.013.89219337
TATATCG800.006300984413.8750015
ACGACGG1601.7854472E-713.8750016
CCGAATT2501.0913936E-1113.3214
Produced by FastQC (version 0.11.5)