FastQCFastQC Report
Fri 10 Feb 2017
ERR1630260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901882
Sequences flagged as poor quality0
Sequence length43
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473795.2533479989621705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354133.926566890125316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293423.253418961682349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149571.6584209464209287No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA86970.9643168396752568No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA48670.539649311107218No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA44590.4944105769934426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.4924147504884231No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.37765472644980164No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.37732208869896505No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33470.3711128506833488No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA30180.3346335773416034No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA22000.24393435061349492No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA21410.23739247484704207No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.23528576909174373No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA20310.22519575731636735No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.22286729306051126No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA19820.21976267405270314No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.19093406898019918No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA16710.18527922721597725No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA15700.17408042293781228No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA15330.1699778906774944No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14670.16265986015908956No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14430.15999875815239686No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA13970.15489831263956927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13550.1502413841278571No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA13400.14857819537367414No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11820.13105927382961408No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11560.12817641332236368No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA11540.12795465482180596No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11240.12462827731344012No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA11200.12418476031232469No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11160.12374124331120923No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11030.12229981305758404No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA10560.11708848829447756No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10540.11686672979391982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10530.11675585054364097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10510.11653409204308324No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA10370.11498178253917918No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10140.11243155978276538No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10130.1123206805324865No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9650.10699847651910116No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA9590.10633320101742799No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9460.1048917707638028No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.10455913301296621No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA9350.10367209901073532No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC9280.1028959442587833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT603.45608E-1133.9166641
CGAATGA303.5988702E-430.83333217
TATTGAC502.7196802E-729.59999830
CCCGTAT502.7196802E-729.5999981
AATGACA250.005495724329.59999819
TTAAAGT250.005495724329.59999830
ACGTGCG405.9387326E-527.7500029
CTACACT405.9387326E-527.75000233
GTATACT556.2592335E-726.909094
ATTGACT556.2592335E-726.9090931
TAGATTG358.867906E-426.4285727
AAGTAAT509.088255E-625.89999834
GTTATAC809.786163E-1025.4375023
ATAGGGG601.3368735E-624.6666663
ACGTTTA451.3229036E-424.66666626
ATCGTCA601.3368735E-624.66666610
GCCTACC751.3729732E-824.6666668
TATACTG751.3729732E-824.6666665
AAGTCTG751.3729732E-824.66666636
TACTGTG1300.024.192317
Produced by FastQC (version 0.11.5)