##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901882
Sequences flagged as poor quality	0
Sequence length	43
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718049589635896	31.0	31.0	34.0	30.0	34.0
2	31.75710902313163	31.0	31.0	34.0	30.0	34.0
3	31.774462734592774	33.0	31.0	34.0	30.0	34.0
4	35.527491401314144	37.0	35.0	37.0	33.0	37.0
5	35.50720160730561	37.0	35.0	37.0	33.0	37.0
6	35.66366109978911	37.0	35.0	37.0	33.0	37.0
7	35.61186385802134	37.0	35.0	37.0	33.0	37.0
8	35.70394020503791	37.0	35.0	37.0	33.0	37.0
9	37.26203982339153	39.0	37.0	39.0	34.0	39.0
10	37.19006810203552	39.0	37.0	39.0	34.0	39.0
11	37.3159836874447	39.0	37.0	39.0	34.0	39.0
12	37.21871819151507	39.0	37.0	39.0	34.0	39.0
13	37.31126023138282	39.0	37.0	39.0	34.0	39.0
14	38.164845290182086	40.0	37.0	41.0	33.0	41.0
15	38.12802450874948	40.0	37.0	41.0	33.0	41.0
16	38.04562237631974	40.0	37.0	41.0	33.0	41.0
17	38.30373929183641	40.0	38.0	41.0	34.0	41.0
18	38.46189634564167	40.0	38.0	41.0	34.0	41.0
19	38.53533499947887	40.0	38.0	41.0	34.0	41.0
20	38.58698698942877	40.0	38.0	41.0	34.0	41.0
21	38.58227351249942	40.0	38.0	41.0	34.0	41.0
22	38.52461962873192	40.0	38.0	41.0	34.0	41.0
23	38.43108965474419	40.0	38.0	41.0	34.0	41.0
24	38.37523090603871	40.0	38.0	41.0	34.0	41.0
25	38.306960334057	40.0	37.0	41.0	34.0	41.0
26	37.976149873265015	40.0	37.0	41.0	33.0	41.0
27	37.75631180132213	40.0	36.0	41.0	33.0	41.0
28	37.57070437152532	39.0	35.0	41.0	33.0	41.0
29	37.32419651351285	39.0	35.0	41.0	33.0	41.0
30	36.956340186410195	38.0	35.0	40.0	33.0	41.0
31	36.48496255607718	38.0	35.0	40.0	32.0	41.0
32	36.10838446714759	37.0	35.0	40.0	31.0	41.0
33	35.818596002581266	37.0	35.0	40.0	31.0	41.0
34	35.55836129338428	37.0	35.0	40.0	30.0	41.0
35	35.276323288412456	36.0	35.0	40.0	30.0	41.0
36	34.89337629534684	35.0	34.0	40.0	27.0	41.0
37	34.5316892897297	35.0	34.0	40.0	25.0	41.0
38	34.12413264706469	35.0	33.0	40.0	23.0	41.0
39	33.67119312726055	35.0	33.0	40.0	21.0	41.0
40	33.16603502453758	35.0	33.0	40.0	18.0	41.0
41	32.58823438099441	35.0	33.0	40.0	15.0	41.0
42	32.04240909564666	35.0	32.0	40.0	10.0	41.0
43	31.002748696614415	35.0	30.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	42.0
11	43.0
12	21.0
13	25.0
14	18.0
15	12.0
16	17.0
17	18.0
18	55.0
19	108.0
20	245.0
21	491.0
22	880.0
23	1460.0
24	2738.0
25	4293.0
26	6780.0
27	9735.0
28	14021.0
29	18545.0
30	23426.0
31	28390.0
32	35247.0
33	44467.0
34	56935.0
35	71512.0
36	100213.0
37	174749.0
38	159777.0
39	147609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.10546612528025	18.38721695299385	15.27794101667402	15.229375905051882
2	16.941462408607777	29.974653003386255	32.234261244819166	20.849623343186803
3	22.144582107193624	31.165939668382336	31.66312222663275	15.026355997791285
4	12.66673467260684	17.85555094790671	30.833191038295478	38.644523341190975
5	10.942784089271102	46.24585034405831	28.125963263486796	14.685402303183787
6	27.34892147753254	33.852765661139706	19.147072455154888	19.65124040617287
7	22.925615546157925	37.19843615905407	20.959282921712596	18.916665373075414
8	35.1971765707709	29.18441658664881	19.181334143491053	16.43707269908924
9	30.372709511887365	10.588968401631256	22.17429774626836	36.86402434021302
10	21.30433914858041	33.55505487414096	24.52105707842046	20.619548898858163
11	29.26912833386186	28.928285518504637	17.195708529497207	24.6068776181363
12	20.222268545109007	36.073122647973904	27.36422281407102	16.34038599284607
13	33.14779538786671	21.040446532916725	27.13814002275242	18.67361805646415
14	23.325667881164055	25.0333192147088	32.11440077526772	19.52661212885943
15	27.23604640074866	28.227639535992516	28.088486076892544	16.44782798636629
16	17.128515703828217	27.774476040102808	31.700932050977844	23.39607620509113
17	17.096915117498742	31.278925624416498	31.95284970761142	19.671309550473342
18	15.078801883173188	24.935412836712565	37.4626614124686	22.523123867645655
19	16.050769391117687	28.9746330451212	40.518160912403175	14.456436651357937
20	17.524021989572915	26.46288538855416	41.36683069403758	14.646261927835349
21	19.50532331280589	26.44802756901679	38.94101445643665	15.105634661740671
22	19.13509749612477	28.10456356818298	36.91768989734799	15.84264903834426
23	17.120199760057304	29.4944349704285	37.505128165325395	15.880237104188796
24	15.417870630525945	30.887521870932115	38.16696641023992	15.527641088302016
25	15.329499868053691	31.078566819162596	37.77766936251084	15.814263950272874
26	16.448049744866843	30.89483990145052	37.70448905732679	14.952621296355842
27	14.933771823808437	31.850175521853192	37.978582564016136	15.237470090322237
28	14.09120040093937	33.46635147391787	38.349473656198924	14.092974468943831
29	14.928449619795051	33.1856052122118	37.79574268030629	14.09020248768686
30	13.60122499395708	35.14195870413202	38.53796838167299	12.718847920237902
31	14.351766639094693	34.25337239239723	37.4169791613537	13.977881807154372
32	12.596658986430597	35.016443392816356	37.666124836730305	14.720772784022742
33	13.096059129686587	34.94514803488705	38.48629865104304	13.472494184383324
34	12.629368365262861	35.27523556296722	36.96847259397571	15.126923477794213
35	12.990280324920555	36.009810596064675	36.690387434276325	14.309521644738446
36	12.578696547885421	36.1728030939746	36.25219263717426	14.996307720965715
37	12.975533384633467	36.624636038860956	35.823311697095626	14.576518879409944
38	12.92275486150073	35.627166303352325	35.863449985696576	15.58662884945037
39	13.226453128014528	34.985729840489114	36.90726724782178	14.880549783674581
40	12.905125060706391	35.76188459244114	36.21471545058001	15.11827489627246
41	12.484005668147274	35.34885938515238	36.61587657808893	15.551258368611414
42	12.196717530674745	35.63914126238244	36.21072379756997	15.953417409372845
43	12.144049886792285	35.91667202583043	35.84193941114248	16.097338676234806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	349.0
2	545.0
3	2226.5
4	3908.0
5	3908.0
6	5954.5
7	8001.0
8	7633.5
9	7266.0
10	10099.5
11	12933.0
12	12933.0
13	25828.0
14	38723.0
15	64772.5
16	90822.0
17	75259.0
18	59696.0
19	59696.0
20	64928.0
21	70160.0
22	47861.0
23	25562.0
24	25968.0
25	26374.0
26	26374.0
27	26452.5
28	26531.0
29	25500.5
30	24470.0
31	23786.0
32	23102.0
33	23102.0
34	22665.5
35	22229.0
36	21767.0
37	21305.0
38	23438.5
39	25572.0
40	25572.0
41	29259.5
42	32947.0
43	37105.0
44	41263.0
45	48704.0
46	56145.0
47	56145.0
48	63496.0
49	70847.0
50	60407.0
51	49967.0
52	45410.0
53	40853.0
54	40853.0
55	37836.0
56	34819.0
57	31323.0
58	27827.0
59	23949.0
60	20071.0
61	20071.0
62	16956.5
63	13842.0
64	11313.5
65	8785.0
66	7473.0
67	6161.0
68	6161.0
69	5484.0
70	4807.0
71	3842.5
72	2878.0
73	2293.5
74	1709.0
75	1709.0
76	1305.0
77	901.0
78	667.5
79	434.0
80	302.5
81	171.0
82	171.0
83	118.0
84	65.0
85	45.5
86	26.0
87	17.0
88	8.0
89	8.0
90	5.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	901882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.605424032874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56795498847535	14.334772116374268
2	12.011261881479388	4.9499428847555755
3	5.167694370838529	3.194476013502718
4	2.8790170783768234	2.3729347079136196
5	1.8169974316537205	1.8720001272933953
6	1.3358638293384162	1.6515624392218136
7	0.9221348073026745	1.3300685103960914
8	0.6784382298070879	1.118360592422997
9	0.5627834512163044	1.043675248589659
>10	4.063784367973226	16.87338143171161
>50	0.5514821927873915	8.04333141643423
>100	0.38490546006859067	15.442618701497832
>500	0.03557949630886133	5.118473530421937
>1k	0.01940699798665163	7.571017576043282
>5k	5.390832774069897E-4	0.9660661970546146
>10k+	0.002156333109627959	14.117318506366336
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47379	5.2533479989621705	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35413	3.926566890125316	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29342	3.253418961682349	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14957	1.6584209464209287	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	8697	0.9643168396752568	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	4867	0.539649311107218	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4459	0.4944105769934426	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4441	0.4924147504884231	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3406	0.37765472644980164	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3403	0.37732208869896505	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3347	0.3711128506833488	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	3018	0.3346335773416034	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	2200	0.24393435061349492	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2141	0.23739247484704207	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2122	0.23528576909174373	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	2031	0.22519575731636735	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2010	0.22286729306051126	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1982	0.21976267405270314	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1722	0.19093406898019918	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	1671	0.18527922721597725	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1570	0.17408042293781228	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	1533	0.1699778906774944	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1467	0.16265986015908956	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1443	0.15999875815239686	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	1397	0.15489831263956927	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1355	0.1502413841278571	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1340	0.14857819537367414	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1182	0.13105927382961408	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1156	0.12817641332236368	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	1154	0.12795465482180596	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	1124	0.12462827731344012	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	1120	0.12418476031232469	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1116	0.12374124331120923	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	1103	0.12229981305758404	No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA	1056	0.11708848829447756	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1054	0.11686672979391982	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	1053	0.11675585054364097	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1051	0.11653409204308324	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	1037	0.11498178253917918	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1014	0.11243155978276538	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1013	0.1123206805324865	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	965	0.10699847651910116	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	959	0.10633320101742799	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	946	0.1048917707638028	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	943	0.10455913301296621	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	935	0.10367209901073532	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	928	0.1028959442587833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.1087925027886131E-4	0.0
6	0.0	0.0	0.0	1.1087925027886131E-4	0.0
7	0.0	0.0	0.0	2.2175850055772262E-4	0.0
8	0.0	0.0	0.0	2.2175850055772262E-4	0.0
9	0.0	0.0	0.0	2.2175850055772262E-4	0.0
10	0.0	0.0	0.0	2.2175850055772262E-4	0.0
11	0.0	0.0	0.0	8.870340022308905E-4	0.0
12	0.0	0.0	0.0	0.0019958265050195037	0.0
13	0.0	0.0	0.0	0.00255022275641381	0.0
14	0.0	0.0	0.0	0.0037698945094812846	0.0
15	0.0	0.0	0.0	0.006098358765337372	0.0
16	0.0	0.0	0.0	0.013970785535136525	0.0
17	0.0	0.0	0.0	0.022397608556329985	0.0
18	0.0	0.0	0.0	0.024836952062464935	0.0
19	0.0	0.0	0.0	0.032487620331706366	0.0
20	0.0	0.0	0.0	0.037366307343976266	0.0
21	0.0	0.0	0.0	0.04878687012269898	0.0
22	0.0	0.0	0.0	0.07229327118181758	0.0
23	0.0	0.0	0.0	0.10533528776491825	0.0
24	0.0	0.0	0.0	0.15378952013678066	0.0
25	0.0	0.0	0.0	0.16798206417247488	0.0
26	0.0	0.0	0.0	0.18583362346737156	0.0
27	0.0	0.0	0.0	0.2095617830270479	0.0
28	0.0	0.0	0.0	0.2475933658726973	0.0
29	0.0	0.0	0.0	0.31556234629363933	0.0
30	0.0	0.0	0.0	0.4734543986907378	0.0
31	0.0	0.0	0.0	0.8201738143127372	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	60	3.45608E-11	33.916664	1
CGAATGA	30	3.5988702E-4	30.833332	17
TATTGAC	50	2.7196802E-7	29.599998	30
CCCGTAT	50	2.7196802E-7	29.599998	1
AATGACA	25	0.0054957243	29.599998	19
TTAAAGT	25	0.0054957243	29.599998	30
ACGTGCG	40	5.9387326E-5	27.750002	9
CTACACT	40	5.9387326E-5	27.750002	33
GTATACT	55	6.2592335E-7	26.90909	4
ATTGACT	55	6.2592335E-7	26.90909	31
TAGATTG	35	8.867906E-4	26.428572	7
AAGTAAT	50	9.088255E-6	25.899998	34
GTTATAC	80	9.786163E-10	25.437502	3
ATAGGGG	60	1.3368735E-6	24.666666	3
ACGTTTA	45	1.3229036E-4	24.666666	26
ATCGTCA	60	1.3368735E-6	24.666666	10
GCCTACC	75	1.3729732E-8	24.666666	8
TATACTG	75	1.3729732E-8	24.666666	5
AAGTCTG	75	1.3729732E-8	24.666666	36
TACTGTG	130	0.0	24.19231	7
>>END_MODULE