FastQCFastQC Report
Fri 10 Feb 2017
ERR1630259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483524
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75581.5631075189649326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66731.3800762733597505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48711.00739570321225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.5672934538926713No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19840.4103208941024644No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG19350.40018696072997406No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11950.24714388530869205No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11720.24238714107262513No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9740.20143777764909293No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.19874918308088121No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.171035977531622No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.15097492575342691No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7110.14704544138450212No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.14642499648414556No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6410.13256839370951598No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.1292593542409477No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6110.1263639447059505No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5590.11560956643310363No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5560.11498912153274708No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC5430.11230052696453537No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5320.11002556232989469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTA352.3854684E-531.71428516
ATCTGAC250.00549323429.5999987
ATTAGAC454.001946E-628.7777773
GACTCTT405.933123E-527.7515
CTTAATA556.249138E-726.90909433
AGACTAT358.861936E-426.428576
CGACTAT358.861936E-426.4285736
TCCGTAT509.076872E-625.89999813
GCTCGGA701.9181243E-723.78571311
TTAGACT551.8997036E-523.5454564
TGTAGAC400.001929578423.12527
CAGTCGG802.7175702E-823.12510
TGCGTCG400.001929578423.12510
GCCCTTT903.814421E-922.6111115
TGACTCT502.698966E-422.19999914
TAGAAAG855.1650204E-821.7647067
TGTACTG855.1650204E-821.7647065
TAATATG603.72054E-521.58333435
CACATCC603.72054E-521.5833349
TACTGAT603.72054E-521.5833347
Produced by FastQC (version 0.11.5)