##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483524
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752721684962896	31.0	31.0	34.0	30.0	34.0
2	31.91837633705876	33.0	31.0	34.0	30.0	34.0
3	32.00022336016413	33.0	31.0	34.0	30.0	34.0
4	35.683626872709524	37.0	35.0	37.0	33.0	37.0
5	35.63290136580604	37.0	35.0	37.0	33.0	37.0
6	35.73525822916753	37.0	35.0	37.0	35.0	37.0
7	35.715726623704306	37.0	35.0	37.0	33.0	37.0
8	35.76483070126819	37.0	35.0	37.0	35.0	37.0
9	37.456109727748775	39.0	37.0	39.0	35.0	39.0
10	37.35495445934431	39.0	37.0	39.0	34.0	39.0
11	37.43576120316675	39.0	37.0	39.0	34.0	39.0
12	37.374093116370645	39.0	37.0	39.0	34.0	39.0
13	37.41068075214467	39.0	37.0	39.0	34.0	39.0
14	38.53430646669038	40.0	38.0	41.0	34.0	41.0
15	38.522393924603534	40.0	38.0	41.0	34.0	41.0
16	38.418084727955595	40.0	38.0	41.0	34.0	41.0
17	38.495127439382536	40.0	38.0	41.0	34.0	41.0
18	38.50450856627592	40.0	38.0	41.0	34.0	41.0
19	38.56882595279655	40.0	38.0	41.0	34.0	41.0
20	38.57672214822842	40.0	38.0	41.0	34.0	41.0
21	38.53761964245829	40.0	38.0	41.0	34.0	41.0
22	38.512483351395176	40.0	38.0	41.0	34.0	41.0
23	38.46126976117008	40.0	38.0	41.0	34.0	41.0
24	38.41777450550541	40.0	38.0	41.0	34.0	41.0
25	38.40431291931735	40.0	38.0	41.0	34.0	41.0
26	38.17100909158594	40.0	37.0	41.0	34.0	41.0
27	38.0200672562272	40.0	37.0	41.0	33.0	41.0
28	37.93973205052903	40.0	37.0	41.0	33.0	41.0
29	37.836303885639595	40.0	37.0	41.0	33.0	41.0
30	37.673575665323746	40.0	37.0	41.0	33.0	41.0
31	37.511786384957105	39.0	36.0	41.0	33.0	41.0
32	37.30696925075074	39.0	35.0	41.0	32.0	41.0
33	37.193481605876855	39.0	35.0	41.0	32.0	41.0
34	37.08163607183925	39.0	35.0	41.0	32.0	41.0
35	36.898944002779594	39.0	35.0	41.0	31.0	41.0
36	36.74846750109612	39.0	35.0	41.0	31.0	41.0
37	36.59566846733564	39.0	35.0	41.0	31.0	41.0
38	36.43264036531796	39.0	35.0	41.0	30.0	41.0
39	36.23613926092604	39.0	35.0	41.0	30.0	41.0
40	36.02545685426163	39.0	35.0	41.0	29.0	41.0
41	35.79418188135439	39.0	35.0	41.0	27.0	41.0
42	35.6108424814487	39.0	35.0	40.0	26.0	41.0
43	34.47021450848355	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	3.0
13	4.0
14	1.0
15	4.0
16	2.0
17	2.0
18	12.0
19	36.0
20	75.0
21	159.0
22	316.0
23	505.0
24	937.0
25	1502.0
26	2347.0
27	3345.0
28	4941.0
29	6476.0
30	8646.0
31	11031.0
32	14277.0
33	18413.0
34	24110.0
35	31150.0
36	43472.0
37	70563.0
38	109652.0
39	131536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49070573539266	20.617797668781694	13.600565845749124	22.29093075007652
2	17.63076910349848	23.783100735434022	34.76497547174494	23.821154689322558
3	19.599233957363026	27.72975074660203	30.315765091288128	22.355250204746817
4	13.963112482524135	18.00965412264955	35.557076794533465	32.470156600292846
5	11.931569063790008	40.46955270058984	34.56291724919549	13.035960986424666
6	29.29203100569982	36.702418080591656	17.64462570627311	16.36092520743541
7	25.847320918920257	32.485254092868196	23.913394164508897	17.75403082370265
8	27.932843043985407	33.43846427478264	20.586775423763868	18.041917257468086
9	26.960812700093477	13.857223219529951	21.810499582233767	37.37146449814281
10	18.81581886317949	29.085422853881088	31.92044241857695	20.178315864362474
11	31.198451369528712	25.785896873784964	20.61841811368205	22.397233643004277
12	19.47224956775672	30.914494420132193	28.783679817341024	20.829576194770063
13	30.291154110240647	21.19646594584757	26.213797040064197	22.298582903847585
14	22.473755180714917	23.008578684822265	28.185570933397308	26.33209520106551
15	26.102323772966802	28.066031882595276	24.647794111564266	21.183850232873652
16	21.54081286554545	26.82658978664968	28.974156401750484	22.658440946054384
17	20.551203249476757	29.47133958190286	28.682547298582904	21.294909870037475
18	19.960332889370537	26.40820310884258	30.900224187423998	22.731239814362887
19	20.38699216584906	28.154962318313054	32.657117330266956	18.800928185570935
20	20.413050851664032	27.111994440813692	32.63395405398698	19.841000653535296
21	20.603320621106707	27.52955385875365	31.10600507937558	20.76112044076406
22	21.52861078250511	27.33080467567277	30.109984199336537	21.030600342485585
23	20.365069779369794	27.73099163640274	30.966611791762144	20.937326792465317
24	19.295422771155103	29.420049470140054	30.184851217312897	21.099676541391947
25	20.754502361826923	27.678667449806007	30.590208552212506	20.976621636154565
26	19.98887335478694	28.649456903897217	30.85534533963154	20.5063244016843
27	19.90532010820559	28.909009687213043	30.040080740563035	21.145589464018332
28	19.75413836748538	28.975190476584412	32.19323135976705	19.07743979616317
29	20.404571438025826	28.40851746759209	31.591192991454403	19.595718102927673
30	19.26233237646942	30.547811484021477	30.93000554264111	19.259850596867995
31	20.972071706885284	28.96319520851085	30.102538860532256	19.96219422407161
32	18.537445917886185	29.859531274559277	30.884092620014727	20.71893018753981
33	19.168852011482368	29.78611196135042	30.53829799554934	20.50673803161787
34	19.631910722115137	28.55763105864445	30.981709284337487	20.828748934902922
35	19.43026612949926	29.973279506291313	31.088839437132386	19.507614927077043
36	20.216163003284223	29.244670378305937	29.57516069522919	20.964005923180647
37	19.379389647670024	28.57376262605372	31.317163160463597	20.729684565812658
38	19.570073047046268	29.31416020714587	31.009422489886745	20.106344255921112
39	18.98747528561147	28.31317576790397	31.934505836318362	20.764843110166197
40	19.15478859374095	28.637254820856878	32.16924082361992	20.03871576178225
41	18.754394818044194	28.209975099477997	31.427809167693848	21.60782091478396
42	18.038608217999517	29.124304067636768	31.51343056394305	21.32365715042066
43	18.833604950323046	27.66377677219745	31.95911681736584	21.543501460113664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	188.0
1	199.0
2	210.0
3	570.0
4	930.0
5	930.0
6	1248.0
7	1566.0
8	1546.0
9	1526.0
10	2206.0
11	2886.0
12	2886.0
13	5481.0
14	8076.0
15	11945.5
16	15815.0
17	13218.0
18	10621.0
19	10621.0
20	11699.0
21	12777.0
22	9232.0
23	5687.0
24	5842.5
25	5998.0
26	5998.0
27	6691.5
28	7385.0
29	8620.5
30	9856.0
31	11095.0
32	12334.0
33	12334.0
34	15244.0
35	18154.0
36	20363.5
37	22573.0
38	25380.0
39	28187.0
40	28187.0
41	32202.0
42	36217.0
43	39148.5
44	42080.0
45	42545.5
46	43011.0
47	43011.0
48	49110.5
49	55210.0
50	52235.0
51	49260.0
52	39530.5
53	29801.0
54	29801.0
55	25897.5
56	21994.0
57	18289.5
58	14585.0
59	12417.5
60	10250.0
61	10250.0
62	8602.0
63	6954.0
64	5381.0
65	3808.0
66	3041.0
67	2274.0
68	2274.0
69	1785.5
70	1297.0
71	1035.0
72	773.0
73	664.5
74	556.0
75	556.0
76	447.5
77	339.0
78	267.5
79	196.0
80	141.0
81	86.0
82	86.0
83	56.5
84	27.0
85	21.5
86	16.0
87	15.0
88	14.0
89	14.0
90	9.5
91	5.0
92	3.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	483524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.96732574336261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.08686995335636	17.680339309247728
2	11.61245143533868	6.030886182008365
3	5.013691682925828	3.9057649532196863
4	2.9399168522577757	3.0536711424391565
5	1.8834359727723338	2.445389771087312
6	1.3517085114793088	2.1060153136595408
7	1.0319774985098018	1.875838710453717
8	0.8108154361584747	1.6843766838778993
9	0.6535818723384489	1.5274596041072408
>10	5.716187325276169	30.315114722219676
>50	0.6408064401492732	11.378423208570247
>100	0.24179869410364857	10.306871615725417
>500	0.010374201397186242	1.860450628613591
>1k	0.004788092952547496	2.8804036241622764
>5k	0.0015960309841824988	2.948994530608155
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7558	1.5631075189649326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6673	1.3800762733597505	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4871	1.00739570321225	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2743	0.5672934538926713	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	1984	0.4103208941024644	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	1935	0.40018696072997406	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	1195	0.24714388530869205	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	1172	0.24238714107262513	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	974	0.20143777764909293	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	961	0.19874918308088121	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.171035977531622	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	730	0.15097492575342691	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	711	0.14704544138450212	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.14642499648414556	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	641	0.13256839370951598	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.1292593542409477	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	611	0.1263639447059505	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	559	0.11560956643310363	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	556	0.11498912153274708	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	543	0.11230052696453537	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	532	0.11002556232989469	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.0681496678551632E-4	0.0
10	0.0	0.0	0.0	2.0681496678551632E-4	0.0
11	0.0	0.0	0.0	2.0681496678551632E-4	0.0
12	0.0	0.0	0.0	6.20444900356549E-4	0.0
13	0.0	0.0	0.0	6.20444900356549E-4	0.0
14	0.0	0.0	0.0	8.272598671420653E-4	0.0
15	0.0	0.0	0.0	8.272598671420653E-4	0.0
16	0.0	0.0	0.0	0.0016545197342841306	0.0
17	0.0	0.0	0.0	0.0028954095349972286	0.0
18	0.0	0.0	0.0	0.003722669402139294	0.0
19	0.0	0.0	0.0	0.004343114302495843	0.0
20	0.0	0.0	0.0	0.005997634036779974	0.0
21	0.0	0.0	0.0	0.007445338804278588	0.0
22	0.0	0.0	0.0	0.010961193239632365	0.0
23	0.0	0.0	0.0	0.014890677608557177	0.0
24	0.0	0.0	0.0	0.022956461313192313	0.0
25	0.0	0.0	0.0	0.025645055881404026	0.0
26	0.0	0.0	0.0	0.03495172938675226	0.0
27	0.0	0.0	0.0	0.0506696668624515	0.0
28	0.0	0.0	0.0	0.0823123567806355	0.0
29	0.0	0.0	0.0	0.12884572430737667	0.0
30	0.0	0.0	0.0	0.23121913286620727	0.0
31	0.0	0.0	0.0	0.5461983272805486	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTTA	35	2.3854684E-5	31.714285	16
ATCTGAC	25	0.005493234	29.599998	7
ATTAGAC	45	4.001946E-6	28.777777	3
GACTCTT	40	5.933123E-5	27.75	15
CTTAATA	55	6.249138E-7	26.909094	33
AGACTAT	35	8.861936E-4	26.42857	6
CGACTAT	35	8.861936E-4	26.42857	36
TCCGTAT	50	9.076872E-6	25.899998	13
GCTCGGA	70	1.9181243E-7	23.785713	11
TTAGACT	55	1.8997036E-5	23.545456	4
TGTAGAC	40	0.0019295784	23.125	27
CAGTCGG	80	2.7175702E-8	23.125	10
TGCGTCG	40	0.0019295784	23.125	10
GCCCTTT	90	3.814421E-9	22.61111	15
TGACTCT	50	2.698966E-4	22.199999	14
TAGAAAG	85	5.1650204E-8	21.764706	7
TGTACTG	85	5.1650204E-8	21.764706	5
TAATATG	60	3.72054E-5	21.583334	35
CACATCC	60	3.72054E-5	21.583334	9
TACTGAT	60	3.72054E-5	21.583334	7
>>END_MODULE