FastQCFastQC Report
Fri 10 Feb 2017
ERR1630258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592901
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT36970.1425816103275829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32540.1254964998663659No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA30670.11828450064233073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.11076396669213365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC250.005497629.63
ATTAGCG502.7033704E-422.225
GGTATCA11300.021.1194691
TCTAGAC7750.020.767743
GTCGTAT450.003827084320.5555571
GTTCTAG8300.020.5060231
GTCTAGA7550.019.8476831
ACTGATC7600.018.7434228
CGTATCC609.241083E-418.53
TAGAACT9350.018.2032074
ACATGTA8950.017.983248
CTCTAGT8600.017.63953427
CTAGACA9350.017.6096254
CGGAGTC7100.017.45774727
CTAGAAC8400.017.398813
GCAATTA7650.016.68627417
GCCTCTA9000.016.6525
ACGGAGT7450.016.63758326
CAACGGA7400.016.524
CCTCTAG11850.016.23628826
Produced by FastQC (version 0.11.5)