FastQCFastQC Report
Fri 10 Feb 2017
ERR1630257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333932
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79282.3741360516512344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71372.137261478384821No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49231.474252242971623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.7369763904028365No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8310.2488530599044117No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.246756824742762No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.24645736257681203No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6590.1973455673610196No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.1605117209491753No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.14913215864307702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.13715367200507889No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.13715367200507889No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4410.13206281518392968No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4270.12787034486063031No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4200.12577410969898062No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4080.1221805637075812No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3780.11319669872908256No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.10990261490363307No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3650.10930369057173316No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3650.10930369057173316No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3600.1078063797419834No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3400.10181713642298432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCT200.00184006637.035
TAGCCGG200.00184006637.05
GACCATT303.5940585E-430.83333418
AGAGACA405.9277045E-527.75000216
GTACTAG556.2394065E-726.909091
CGACGAG509.0658905E-625.89999824
TGCGACG601.3326626E-624.66666822
ATCGTCA551.8974215E-523.54545410
CAATGCG551.8974215E-523.54545419
AGTCCGC400.001928333723.12500234
CAAGTCC400.001928333723.12500232
GCCGGAA400.001928333723.12500215
TATAAGA502.6965427E-422.19999912
TAGGCAT603.716103E-521.5833345
CTAGGCA705.0840554E-621.1428574
CGTATCC450.0038202120.5555553
CCGCTTG450.0038202120.5555557
CTGAACG450.0038202120.55555524
GTTCGAC450.0038202120.55555515
GTCCGCT450.0038202120.55555535
Produced by FastQC (version 0.11.5)