FastQCFastQC Report
Fri 10 Feb 2017
ERR1630256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494758
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86231.74287227290918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83791.693555233063437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54321.0979104936150603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.7041826509121631No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.2637653155684193No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.23041567796781456No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10970.22172456029008122No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10120.20454444395037577No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.17644990075956327No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7840.15846130835681282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.14977019067907948No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5330.10772943540074138No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.10429341213280027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACT200.00184092337.034
CCGGACA200.00184092337.013
CTCTCCG200.00184092337.09
TCCGGAC251.2326098E-436.99999612
ACAAATC251.2326098E-436.99999620
CTGTACC250.005493356429.5999982
GACAATG250.005493356429.59999816
GGACAAT250.005493356429.59999815
TCTGGTA604.3122782E-827.74999812
TTAGACT856.91216E-1126.1176454
CTCTATA451.3217243E-424.6666682
AGACTAT751.369699E-824.6666666
ATTAGAC952.8012437E-1023.3684223
ATCCTTT652.6777197E-622.7692333
AATGCAC652.6777197E-622.7692328
CCCTACA502.6990895E-422.19999916
ACTCTCC502.6990895E-422.1999998
CAGGTGA855.165748E-821.76470621
TTTACAC603.7207683E-521.5833323
AATTCAA603.7207683E-521.58333229
Produced by FastQC (version 0.11.5)