FastQCFastQC Report
Fri 10 Feb 2017
ERR1630255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196620
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55082.8013426914861155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47722.4270165802054726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35221.7912725053402503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.7700132234767572No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6210.3158376563930424No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.27108127352253075No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.22530770013223478No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.2247991048723426No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4260.21666158071406774No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.2085240565557929No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3760.19123181771945885No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.1764825551825857No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3380.1719051978435561No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2690.1368121249109958No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2600.13223476757196623No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2570.13070898179228968No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.12969179127250535No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2540.12918319601261316No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2520.1281660054928288No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.12714881497304445No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2410.12257145763401485No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.12206286237412267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2370.12053707659444614No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2200.11189095717627912No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT2130.10833079035703387No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2100.10680500457735734No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2080.10578781405757298No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2060.10477062353778863No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2030.10324483775811209No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA2000.10171905197843556No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.10121045671854338No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1990.10121045671854338No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1970.10019326619875903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAA200.001838227637.02
ATACTTT200.001838227637.030
GTACTGT251.2299066E-437.06
TTAGGTC352.3783237E-531.71428520
ATTAGGT352.3783237E-531.71428519
CAATTAG352.3783237E-531.71428517
TAGTACT352.3783237E-531.7142854
ACTGTGC303.5887293E-430.8333348
TCAAAAG250.005485404729.63
CGTATAG250.005485404729.61
TTTCGTC250.005485404729.620
GCAGTTA250.005485404729.613
GCTCTAG250.005485404729.61
TACTGTG502.7014175E-729.67
AAAGGGT250.005485404729.66
CTAGTAC405.9154965E-527.753
ATGCGAC405.9154965E-527.7521
ACTGCGC358.8431593E-426.428578
CTGTGCG358.8431593E-426.428579
CATTGCG451.3177698E-424.66666429
Produced by FastQC (version 0.11.5)