FastQCFastQC Report
Fri 10 Feb 2017
ERR1630254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400731
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC24400.6088872585350272No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT10730.2677606673803624No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG8820.22009777132290737No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT7150.17842393026743625No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG7110.1774257544337723No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG6630.16544764442980453No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG6450.16095585317831662No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG6250.15596497400999673No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6150.1534695344258368No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT6100.1522218146337568No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT5830.14548412775652494No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT5600.13974461671295707No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG5480.13675008921196513No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT5150.1285151385842373No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG5070.12651878691690935No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC5020.12527106712482938No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG5000.12477197920799739No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG4810.12003064399809349No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC4660.11628748462185357No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT4290.10705435816046176No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA4260.10630572628521377No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT4240.10580663836838179No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA4080.10181393503372588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC303.5953327E-430.8333323
TAATAGA250.005492124729.64
GGTTAAT901.4188117E-1024.6666681
CGAACGA400.001929004623.12516
ACGAACG400.001929004623.12515
TGCATCA5000.022.93999914
GCTGCAT5300.021.64150812
GCATCAG5300.021.64150815
CAGGACA5700.021.4210534
TCCGATA450.003821524820.5555578
ATTCGTA450.003821524820.55555724
TTCGTAT450.003821524820.55555725
TAACGAA450.003821524820.55555713
GTTAATG450.003821524820.5555572
TTTATAC450.003821524820.5555573
CATCAGA5700.020.44736916
GACAGGC5800.020.4137947
CCAGGAC6000.020.353
ACAGGCT5950.020.2100838
CTAGAAC555.135061E-420.1818183
Produced by FastQC (version 0.11.5)