##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400731
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.771093326945007	31.0	31.0	34.0	30.0	34.0
2	31.99677339661768	33.0	31.0	34.0	30.0	34.0
3	32.10471862670969	34.0	31.0	34.0	30.0	34.0
4	35.76578053607033	37.0	35.0	37.0	33.0	37.0
5	35.68761089109652	37.0	35.0	37.0	33.0	37.0
6	35.74416004751317	37.0	35.0	37.0	35.0	37.0
7	35.70943850113917	37.0	35.0	37.0	33.0	37.0
8	35.70182741040748	37.0	35.0	37.0	33.0	37.0
9	37.46118468498818	39.0	37.0	39.0	35.0	39.0
10	37.342354347430074	39.0	37.0	39.0	34.0	39.0
11	37.423049377263055	39.0	37.0	39.0	34.0	39.0
12	37.3277934574565	39.0	37.0	39.0	34.0	39.0
13	37.315643162121226	39.0	37.0	39.0	34.0	39.0
14	38.54932361110071	40.0	38.0	41.0	34.0	41.0
15	38.56317080535322	40.0	38.0	41.0	34.0	41.0
16	38.52448899635915	40.0	38.0	41.0	34.0	41.0
17	38.478141196962554	40.0	38.0	41.0	34.0	41.0
18	38.37018598511221	40.0	38.0	41.0	34.0	41.0
19	38.39192625476941	40.0	38.0	41.0	34.0	41.0
20	38.357988276424834	40.0	38.0	41.0	34.0	41.0
21	38.30373242898603	40.0	38.0	41.0	34.0	41.0
22	38.25963801153392	40.0	38.0	41.0	34.0	41.0
23	38.19765129226339	40.0	38.0	41.0	33.0	41.0
24	38.14597822479419	40.0	38.0	41.0	33.0	41.0
25	38.11414390201906	40.0	38.0	41.0	33.0	41.0
26	37.908946400453175	40.0	37.0	41.0	33.0	41.0
27	37.797619849724626	40.0	37.0	41.0	32.0	41.0
28	37.6927340285628	40.0	37.0	41.0	32.0	41.0
29	37.622584726412484	40.0	37.0	41.0	32.0	41.0
30	37.55046153155109	40.0	37.0	41.0	32.0	41.0
31	37.519692761478396	40.0	37.0	41.0	32.0	41.0
32	37.440712098639736	40.0	37.0	41.0	31.0	41.0
33	37.333675208556365	40.0	37.0	41.0	31.0	41.0
34	37.25585492512433	40.0	36.0	41.0	31.0	41.0
35	37.09395330034362	39.0	36.0	41.0	31.0	41.0
36	37.00215605980071	39.0	36.0	41.0	30.0	41.0
37	36.928932376082706	39.0	36.0	41.0	30.0	41.0
38	36.817461089858284	39.0	36.0	41.0	30.0	41.0
39	36.73788900783818	39.0	35.0	41.0	30.0	41.0
40	36.60162802478471	39.0	35.0	41.0	30.0	41.0
41	36.47754977778111	39.0	35.0	40.0	30.0	41.0
42	36.45751389335989	39.0	35.0	40.0	30.0	41.0
43	35.05915938622168	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	3.0
17	6.0
18	15.0
19	32.0
20	88.0
21	185.0
22	326.0
23	542.0
24	887.0
25	1372.0
26	1952.0
27	2913.0
28	3924.0
29	5257.0
30	7041.0
31	9055.0
32	11516.0
33	14847.0
34	19481.0
35	25585.0
36	34148.0
37	50922.0
38	93226.0
39	117405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61554259590598	16.554247113400262	11.110695204513751	28.71951508618001
2	20.464351397820483	18.525394840928204	32.53129905098432	28.478954710266986
3	20.83143056065041	20.467845013238307	27.732069642727915	30.968654783383364
4	15.88946200818005	14.183829052406727	31.440292864789598	38.48641607462363
5	16.65406469676666	35.123312147051266	32.091103508338506	16.131519647843565
6	39.0179447060497	31.54410315149065	14.362252982674162	15.075699159785492
7	32.9038681808994	28.046744574290482	19.155493335928593	19.89389390888152
8	29.8786467730223	31.709301251962042	18.059496270565543	20.35255570445012
9	28.498419139023433	12.941599227411904	16.549006690273522	42.01097494329113
10	18.576301808445066	25.096386353938176	30.681679231204974	25.645632606411784
11	39.53824385934704	19.52007705917436	19.060916175688927	21.88076290578967
12	24.777469175082537	22.487903356615785	26.91057093162246	25.82405653667922
13	32.1751998223247	17.1945769106957	22.724720573152563	27.90550269382703
14	24.363226204111985	17.83041491673966	23.140710351832027	34.66564852731633
15	28.130341800359844	24.6239996406567	19.440472536439657	27.805186022543797
16	27.348520578642532	23.63580556532936	20.436152930519473	28.579520925508632
17	26.35433744831321	24.305831093676307	22.730210540237717	26.60962091777277
18	26.41148301479047	22.159753051298754	23.7331277091116	27.69563622479918
19	27.390693507614834	22.545298467051463	22.426765086803865	27.637242938529837
20	27.1062133950206	23.18038784122017	22.53631488454849	27.177083879210745
21	29.255285964899148	22.28327731071467	21.78044623450644	26.680990489879747
22	28.49916777089868	22.887173690081376	21.93691029643327	26.676748242586672
23	27.48751656348024	22.965280949065583	21.89623463121146	27.650967856242715
24	27.829890874426983	22.690283506891156	22.320209816560237	27.15961580212162
25	27.68939762583878	22.30997851426518	22.652851913128757	27.347771946767285
26	28.648395058031443	22.468688471817753	22.78361294733874	26.09930352281206
27	28.86499921393653	22.262814706124555	21.63396393091625	27.23822214902266
28	26.962226531014572	21.93017260955604	23.07682709847753	28.03077376095186
29	26.739882864065915	22.206417771522542	23.709670577020496	27.344028787391046
30	26.539249521499457	23.042140488257708	23.137216736414203	27.28139325382863
31	26.932281256004654	22.728962820445638	22.932341146554673	27.406414776995042
32	26.410983926873637	22.62465344582775	23.128233153911225	27.836129473387384
33	27.376219958026702	22.9218603003012	22.499881466619755	27.20203827505234
34	27.879799666110184	22.96727730073291	22.44847541118606	26.70444762197085
35	27.91673217195575	22.358390042197883	23.31289568313906	26.411982102707306
36	27.51646366265649	23.213078099772666	21.683124090724203	27.587334146846636
37	26.58840968130741	22.92435573988536	22.981501306362624	27.50573327244461
38	27.72308606022494	22.689285331057494	23.128233153911225	26.45939545480634
39	26.144470979285355	22.695274386059477	23.6894575163888	27.470797118266365
40	26.59789235172722	21.707579398648967	24.905235681791527	26.789292567832284
41	25.506137533657242	21.906465933506517	25.00954505640941	27.57785147642683
42	25.769406409786118	22.462449872857356	24.23321380177725	27.53492991557928
43	25.8300455916812	20.307138704018406	25.533587369083	28.329228335217387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	11.0
4	18.0
5	18.0
6	28.0
7	38.0
8	37.0
9	36.0
10	47.0
11	58.0
12	58.0
13	114.5
14	171.0
15	256.0
16	341.0
17	376.0
18	411.0
19	411.0
20	532.0
21	653.0
22	779.0
23	905.0
24	1222.5
25	1540.0
26	1540.0
27	2011.0
28	2482.0
29	3133.0
30	3784.0
31	4628.5
32	5473.0
33	5473.0
34	6730.0
35	7987.0
36	9463.5
37	10940.0
38	12516.5
39	14093.0
40	14093.0
41	15387.0
42	16681.0
43	18018.0
44	19355.0
45	20628.0
46	21901.0
47	21901.0
48	23297.0
49	24693.0
50	26934.5
51	29176.0
52	32031.0
53	34886.0
54	34886.0
55	35798.5
56	36711.0
57	34971.0
58	33231.0
59	30648.0
60	28065.0
61	28065.0
62	27626.0
63	27187.0
64	24069.5
65	20952.0
66	18092.5
67	15233.0
68	15233.0
69	12870.5
70	10508.0
71	9776.0
72	9044.0
73	9365.5
74	9687.0
75	9687.0
76	9022.5
77	8358.0
78	6271.0
79	4184.0
80	2655.5
81	1127.0
82	1127.0
83	763.0
84	399.0
85	305.0
86	211.0
87	156.0
88	101.0
89	101.0
90	92.5
91	84.0
92	49.0
93	14.0
94	9.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	400731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.87024866661924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46996483566474	55.35528972576257
2	5.824721334058482	7.207539147039207
3	1.605989763425343	2.980889580575129
4	0.7625172798955456	1.8870853487892605
5	0.45248528434158114	1.399768853009977
6	0.2979674916938419	1.1061193683400081
7	0.20512144179412453	0.8883640227460564
8	0.15344712966962057	0.7595049655870714
9	0.12732501556746387	0.7089867337176139
>10	0.9202737022787291	11.084890056550112
>50	0.09292140352137271	4.108335658530452
>100	0.08080122045288969	9.46521941962134
>500	0.005656085431702279	2.1698991148422864
>1k	8.080122045288971E-4	0.8781080048889475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2440	0.6088872585350272	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1073	0.2677606673803624	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	882	0.22009777132290737	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	715	0.17842393026743625	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	711	0.1774257544337723	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	663	0.16544764442980453	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	645	0.16095585317831662	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	625	0.15596497400999673	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	615	0.1534695344258368	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	610	0.1522218146337568	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	583	0.14548412775652494	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	560	0.13974461671295707	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	548	0.13675008921196513	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	515	0.1285151385842373	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	507	0.12651878691690935	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	502	0.12527106712482938	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	500	0.12477197920799739	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	481	0.12003064399809349	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	466	0.11628748462185357	No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT	429	0.10705435816046176	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	426	0.10630572628521377	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	424	0.10580663836838179	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	408	0.10181393503372588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	2.4954395841599475E-4	0.0
17	0.0	0.0	0.0	7.486318752479843E-4	0.0
18	0.0	0.0	0.0	7.486318752479843E-4	0.0
19	0.0	0.0	0.0	9.98175833663979E-4	0.0
20	0.0	0.0	0.0	0.0014972637504959685	0.0
21	0.0	0.0	0.0	0.001996351667327958	0.0
22	0.0	0.0	0.0	0.0027449835425759424	0.0
23	0.0	0.0	0.0	0.004242247293071911	0.0
24	0.0	0.0	0.0	0.0049908791683198955	0.0
25	0.0	0.0	0.0	0.0069872308356478535	0.0
26	0.0	0.0	0.0	0.008234950627727828	0.0
27	0.0	0.0	0.0	0.012976285837631728	0.0
28	0.0	0.0	0.0	0.029695731051503377	0.0
29	0.0	0.0	0.0	0.056396934602014816	0.0
30	0.0	0.0	0.0	0.09532579211491	0.0
31	0.0	0.0	0.0	0.18316526547734016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAC	30	3.5953327E-4	30.833332	3
TAATAGA	25	0.0054921247	29.6	4
GGTTAAT	90	1.4188117E-10	24.666668	1
CGAACGA	40	0.0019290046	23.125	16
ACGAACG	40	0.0019290046	23.125	15
TGCATCA	500	0.0	22.939999	14
GCTGCAT	530	0.0	21.641508	12
GCATCAG	530	0.0	21.641508	15
CAGGACA	570	0.0	21.421053	4
TCCGATA	45	0.0038215248	20.555557	8
ATTCGTA	45	0.0038215248	20.555557	24
TTCGTAT	45	0.0038215248	20.555557	25
TAACGAA	45	0.0038215248	20.555557	13
GTTAATG	45	0.0038215248	20.555557	2
TTTATAC	45	0.0038215248	20.555557	3
CATCAGA	570	0.0	20.447369	16
GACAGGC	580	0.0	20.413794	7
CCAGGAC	600	0.0	20.35	3
ACAGGCT	595	0.0	20.210083	8
CTAGAAC	55	5.135061E-4	20.181818	3
>>END_MODULE