FastQCFastQC Report
Fri 10 Feb 2017
ERR1630253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758688
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC27400.3611497743472943No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT21850.287997174068919No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20910.275607364292041No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17370.2289478678982665No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG14690.19362372938546543No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG14210.1872970180100384No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13220.17424817579822008No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA12890.16989856172761397No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA12780.16844869037074528No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG12660.16686701252688851No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA12020.15843139735965245No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG11780.15526804167193894No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG10270.13536526213674133No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG9660.12732506643046945No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG9400.12389809776877979No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9370.12350267830781558No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG9140.12047112910709014No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT9130.12033932262010207No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9090.11981209667214981No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC9060.11941667721118562No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA8990.11849403180226917No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG8960.11809861234130499No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC8910.11743957990636468No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA8810.11612151503648403No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG8800.11598970854949596No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG8200.10808131933021216No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC7980.10518157661647476No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA7940.10465435066852251No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT7850.10346809228562992No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC7810.10294086633767768No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT7640.10070015605888058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA405.9375092E-527.752
GATACAC400.001930585523.1253
GGTATCA4500.023.0222231
ATACACC705.0981507E-621.1428574
CGAAATA450.003824626620.55555724
TACTATC555.1408785E-420.1818187
TCGGTGA555.1408785E-420.18181813
CGCCTTA500.007032730618.525
ATACTAT609.23287E-418.56
ATATACT609.23287E-418.54
ACTCAGT500.007032730618.58
GTATCAA5950.017.4117642
GCTTTAT4900.017.3673481
TACACCT1603.45608E-1117.343755
ACCTAGG7450.017.1342281
CTGCGCG650.001579264817.0769239
TAGGCCT8500.016.5411784
CTAGAGT1352.215711E-816.4444454
TAGACAG904.4427507E-516.4444455
AGGCCCG1801.0913936E-1116.44444510
Produced by FastQC (version 0.11.5)