FastQCFastQC Report
Fri 10 Feb 2017
ERR1630252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700647
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97960.3627278944638081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87760.32495916719215806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74210.2747860049832503No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC51840.1919540021335628No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT47240.17492104669732847No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA42440.1571475279812578No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA41650.15422230302590453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.13507874224213678No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG31250.11571301247441818No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA30360.11241750587914674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.021.0338731
TATACCG909.4902134E-820.5555575
CTAACGC609.2412194E-418.53
ATACCGA801.6179882E-518.56
TCTAGCG3750.018.25333428
AAGACGG5300.018.1509445
CTAGCGG3750.017.76000229
GACGGAC5400.017.129637
GCTTTAT14800.017.1250021
ACGGACC5700.016.8771938
CGCAATA4200.016.73809436
CAAGACG6200.016.411294
CGCAAGA6100.016.073772
CGTATAC700.002593708715.8571423
TTACGCT700.002593708715.8571424
AGACGGA6050.015.5950416
CAATAGT1902.7284841E-1115.5789484
GTCTAAC1557.221388E-915.5161291
TCTTATA22600.015.47123937
CGGTCCA4700.015.35106310
Produced by FastQC (version 0.11.5)