##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2700647
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824619804069172	31.0	31.0	34.0	30.0	34.0
2	32.01496011881597	33.0	31.0	34.0	30.0	34.0
3	32.168332995759904	34.0	31.0	34.0	30.0	34.0
4	35.81968506065398	37.0	35.0	37.0	35.0	37.0
5	35.73503386410738	37.0	35.0	37.0	35.0	37.0
6	35.8000608743016	37.0	35.0	37.0	35.0	37.0
7	35.775158323172185	37.0	35.0	37.0	35.0	37.0
8	35.761367553775074	37.0	35.0	37.0	33.0	37.0
9	37.450957863060225	39.0	37.0	39.0	35.0	39.0
10	37.377253302634514	39.0	37.0	39.0	34.0	39.0
11	37.44199223371288	39.0	37.0	39.0	35.0	39.0
12	37.33218039973384	39.0	37.0	39.0	34.0	39.0
13	37.3692711413228	39.0	37.0	39.0	34.0	39.0
14	38.583701979562676	40.0	38.0	41.0	34.0	41.0
15	38.57275904625818	40.0	38.0	41.0	34.0	41.0
16	38.548319347178655	40.0	38.0	41.0	34.0	41.0
17	38.53741047978503	40.0	38.0	41.0	34.0	41.0
18	38.52244480674446	40.0	38.0	41.0	34.0	41.0
19	38.525752902915485	40.0	38.0	41.0	34.0	41.0
20	38.4857102760931	40.0	38.0	41.0	34.0	41.0
21	38.44106208623341	40.0	38.0	41.0	34.0	41.0
22	38.40827105504718	40.0	38.0	41.0	34.0	41.0
23	38.36744009861341	40.0	38.0	41.0	34.0	41.0
24	38.35237148727694	40.0	38.0	41.0	34.0	41.0
25	38.32879750667155	40.0	38.0	41.0	34.0	41.0
26	38.16604872832325	40.0	38.0	41.0	33.0	41.0
27	38.06312043003029	40.0	38.0	41.0	33.0	41.0
28	37.96862862862122	40.0	37.0	41.0	33.0	41.0
29	37.91149528242676	40.0	37.0	41.0	33.0	41.0
30	37.8483704090168	40.0	37.0	41.0	33.0	41.0
31	37.740453676470864	40.0	37.0	41.0	33.0	41.0
32	37.65704329369962	40.0	37.0	41.0	33.0	41.0
33	37.58747366834688	40.0	37.0	41.0	32.0	41.0
34	37.49791549950808	40.0	37.0	41.0	32.0	41.0
35	37.397789862947654	40.0	37.0	41.0	32.0	41.0
36	37.298342212069926	39.0	36.0	41.0	31.0	41.0
37	37.24396265043154	39.0	36.0	41.0	31.0	41.0
38	37.13555307302287	39.0	36.0	41.0	31.0	41.0
39	37.04070580123948	39.0	36.0	41.0	31.0	41.0
40	36.92310101986672	39.0	36.0	41.0	31.0	41.0
41	36.79954025831588	39.0	35.0	41.0	30.0	41.0
42	36.73245114966895	39.0	35.0	41.0	30.0	41.0
43	35.58017986060377	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	11.0
12	5.0
13	9.0
14	7.0
15	8.0
16	28.0
17	61.0
18	80.0
19	183.0
20	419.0
21	840.0
22	1529.0
23	2605.0
24	4438.0
25	6851.0
26	10400.0
27	15629.0
28	22114.0
29	30989.0
30	41691.0
31	54511.0
32	71739.0
33	93520.0
34	123827.0
35	166046.0
36	234421.0
37	357413.0
38	647548.0
39	813709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.896498505728445	19.25075731852404	12.476491744385697	25.376252431361817
2	18.25688436881977	20.925541175873782	34.332439596881784	26.485134858424665
3	20.084927796931623	22.838268015034917	29.60123999915576	27.475564188877705
4	14.182416287652552	15.226129146089807	35.36908007599661	35.22237449026104
5	14.483603373561966	36.65106916972118	34.34906524251411	14.516262214202744
6	34.752598173696896	35.46716768241092	15.458406818810456	14.32182732508173
7	29.650709626248823	30.560898925331596	21.0475860043908	18.740805444028783
8	28.31499266657212	32.65547107785653	19.872089910306677	19.157446345264674
9	26.901183309036686	14.281207429182711	19.773965275728372	39.04364398605223
10	17.53083612926828	26.735815528649248	32.35583917483477	23.377509167247702
11	36.10531106064584	21.975289625041704	21.148783976580425	20.770615337732032
12	22.729442241062976	25.664183434562162	28.493912754980567	23.112461569394295
13	30.915332511061237	19.262717415493398	24.768805401076115	25.05314467236925
14	22.7967224150361	20.666899450390964	25.12686774687695	31.40951038769599
15	25.382139909436518	28.12326083342251	22.054640980476158	24.439958276664814
16	24.717373281291483	26.56011689050809	23.93848585172368	24.78402397647675
17	23.85439489129827	26.346871694079233	25.101429398214574	24.69730401640792
18	23.697099250661044	24.90058863672298	27.006713576413354	24.39559853620262
19	24.742589460969906	25.03229781604186	26.92995419245833	23.295158530529907
20	25.37069820676305	24.691527622825195	25.779341024576702	24.158433145835055
21	25.399246921200735	24.720668787886755	25.940857875908996	23.939226415003514
22	25.222400409975833	24.84363932050357	26.41741034648364	23.516549923036962
23	24.57088986453987	24.706412944749907	25.833179975020798	24.889517215689423
24	23.983326958317765	25.35999706736941	26.712302644514445	23.944373329798378
25	24.50886768985358	25.38602786665566	25.789597826002435	24.315506617488328
26	24.33635347381572	25.81459183669691	26.02313445629881	23.825920233188565
27	24.576592201794607	24.50679411267004	26.327839217787442	24.588774467747914
28	24.008654222488165	25.32578304384098	26.257078396399088	24.408484337271773
29	24.04264607703265	25.7015448520299	26.604143377494356	23.65166569344309
30	24.315173364012402	25.712579244899462	25.793189557909642	24.179057833178494
31	23.669957606455046	26.26840901458058	26.257263537219046	23.80436984174533
32	23.502738417868013	25.38225099392849	26.052571846672297	25.062438741531196
33	22.92943135478276	25.718614835630127	26.654946018491128	24.697007791095984
34	24.898885341179355	24.36308780821781	26.409227122241447	24.32879972836139
35	24.068158482023012	24.766213429596686	26.66079646840183	24.50483161997847
36	23.10209368347659	25.89912713509022	26.88329870582864	24.11548047560455
37	24.057864652433285	24.753920079151403	27.064033174272684	24.124182094142625
38	23.979587113754594	25.05966162923181	26.68753080280392	24.273220454209678
39	23.922045346911315	24.516162238159968	27.06251501954902	24.4992773953797
40	22.99641530344395	24.34920224672088	27.894797061593017	24.75958538824215
41	22.135547518798273	24.83878863102064	28.26204239206383	24.763621458117257
42	22.940761972964257	24.614953379690128	28.537754101146874	23.906530546198745
43	22.97975262964764	24.326133700553978	28.1459220697855	24.548191600012885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	586.0
1	500.5
2	415.0
3	984.5
4	1554.0
5	1554.0
6	2030.5
7	2507.0
8	2287.0
9	2067.0
10	2936.5
11	3806.0
12	3806.0
13	6978.0
14	10150.0
15	15524.5
16	20899.0
17	20464.5
18	20030.0
19	20030.0
20	23737.5
21	27445.0
22	27236.5
23	27028.0
24	32615.5
25	38203.0
26	38203.0
27	44257.5
28	50312.0
29	58276.5
30	66241.0
31	74162.5
32	82084.0
33	82084.0
34	91149.0
35	100214.0
36	110291.0
37	120368.0
38	127521.0
39	134674.0
40	134674.0
41	140970.0
42	147266.0
43	149592.5
44	151919.0
45	157721.0
46	163523.0
47	163523.0
48	173782.0
49	184041.0
50	181617.0
51	179193.0
52	181980.5
53	184768.0
54	184768.0
55	179355.0
56	173942.0
57	171192.0
58	168442.0
59	166919.0
60	165396.0
61	165396.0
62	151339.0
63	137282.0
64	125460.0
65	113638.0
66	98000.0
67	82362.0
68	82362.0
69	72643.5
70	62925.0
71	52239.0
72	41553.0
73	28845.5
74	16138.0
75	16138.0
76	11854.5
77	7571.0
78	6012.5
79	4454.0
80	3738.5
81	3023.0
82	3023.0
83	2267.5
84	1512.0
85	1370.0
86	1228.0
87	1117.0
88	1006.0
89	1006.0
90	861.5
91	717.0
92	415.0
93	113.0
94	77.0
95	41.0
96	41.0
97	24.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2700647.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.896730762977114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52923526801413	42.597332765205856
2	12.252003788267306	12.961818913899029
3	3.4154444850829253	5.419975420899797
4	1.2751776774633545	2.698109211189502
5	0.6560027593531488	1.7350200670636793
6	0.3649740861002727	1.1583561580745856
7	0.23560243412216464	0.8723818967403538
8	0.16279438495251658	0.6889032600446179
9	0.12541607847392872	0.5970690482745437
>10	0.7912138758863515	8.080557184501798
>50	0.09311850481809981	3.4442116120657875
>100	0.08051926488757566	9.011798025723674
>500	0.01240166093310453	4.4229718351194
>1k	0.005815468121170498	5.1559951550573935
>5k	2.8026352391183123E-4	1.1554994461400871
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9796	0.3627278944638081	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8776	0.32495916719215806	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7421	0.2747860049832503	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	5184	0.1919540021335628	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4724	0.17492104669732847	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4244	0.1571475279812578	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4165	0.15422230302590453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3648	0.13507874224213678	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3125	0.11571301247441818	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	3036	0.11241750587914674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.702816399181381E-5	0.0
10	0.0	0.0	0.0	3.702816399181381E-5	0.0
11	0.0	0.0	0.0	1.1108449197544145E-4	0.0
12	0.0	0.0	0.0	1.8514081995906907E-4	0.0
13	0.0	0.0	0.0	1.8514081995906907E-4	0.0
14	0.0	0.0	0.0	2.221689839508829E-4	0.0
15	0.0	0.0	0.0	4.0730980390995197E-4	0.0
16	0.0	0.0	0.0	6.665069518526487E-4	0.0
17	0.0	0.0	0.0	0.0010738167557626005	0.0
18	0.0	0.0	0.0	0.0011478730837462283	0.0
19	0.0	0.0	0.0	0.0013330139037052973	0.0
20	0.0	0.0	0.0	0.0017032955436234354	0.0
21	0.0	0.0	0.0	0.001999520855557946	0.0
22	0.0	0.0	0.0	0.0028881967913614776	0.0
23	7.405632798362763E-5	0.0	0.0	0.004628520498976727	0.0
24	7.405632798362763E-5	0.0	0.0	0.006479928698567418	0.0
25	1.1108449197544145E-4	0.0	0.0	0.007146435650420066	0.0
26	1.1108449197544145E-4	0.0	0.0	0.009368125489928894	0.0
27	1.1108449197544145E-4	0.0	0.0	0.016958899108250727	0.0
28	1.4811265596725525E-4	3.702816399181381E-5	0.0	0.04284158573852858	0.0
29	1.4811265596725525E-4	7.405632798362763E-5	0.0	0.09153362138776375	0.0
30	1.8514081995906907E-4	7.405632798362763E-5	0.0	0.1546296128298145	0.0
31	1.8514081995906907E-4	7.405632798362763E-5	0.0	0.37191087913377796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3395	0.0	21.033873	1
TATACCG	90	9.4902134E-8	20.555557	5
CTAACGC	60	9.2412194E-4	18.5	3
ATACCGA	80	1.6179882E-5	18.5	6
TCTAGCG	375	0.0	18.253334	28
AAGACGG	530	0.0	18.150944	5
CTAGCGG	375	0.0	17.760002	29
GACGGAC	540	0.0	17.12963	7
GCTTTAT	1480	0.0	17.125002	1
ACGGACC	570	0.0	16.877193	8
CGCAATA	420	0.0	16.738094	36
CAAGACG	620	0.0	16.41129	4
CGCAAGA	610	0.0	16.07377	2
CGTATAC	70	0.0025937087	15.857142	3
TTACGCT	70	0.0025937087	15.857142	4
AGACGGA	605	0.0	15.595041	6
CAATAGT	190	2.7284841E-11	15.578948	4
GTCTAAC	155	7.221388E-9	15.516129	1
TCTTATA	2260	0.0	15.471239	37
CGGTCCA	470	0.0	15.351063	10
>>END_MODULE